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  • 1
    Electronic Resource
    Electronic Resource
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Microbiology 56 (2002), S. 187-209 
    ISSN: 0066-4227
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Biology
    Notes: Abstract Prokaryotic biofilms that predominate in a diverse range of ecosystems are often composed of highly structured multispecies communities. Within these communities metabolic activities are integrated, and developmental sequences, not unlike those of multicellular organisms, can be detected. These structural adaptations and interrelationships are made possible by the expression of sets of genes that result in phenotypes that differ profoundly from those of planktonically grown cells of the same species. Molecular and microscopic evidence suggest the existence of a succession of de facto biofilm phenotypes. We submit that complex cell-cell interactions within prokaryotic communities are an ancient characteristic, the development of which was facilitated by the localization of cells at surfaces. In addition to spatial localization, surfaces may have provided the protective niche in which attached cells could create a localized homeostatic environment. In a holistic sense both biofilm and planktonic phenotypes may be viewed as integrated components of prokaryote life.
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  • 2
    ISSN: 1365-2494
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Eight multiparous Holstein–Friesian dairy cows in late lactation were used to investigate the potential of using perennial ryegrass with a high concentration of water-soluble carbohydrate (WSC) to increase the efficiency of milk production. After a pretreatment period on a common pasture, the cows were each given ad libitum access to one of two varieties of zero-grazed grass continuously for 3 weeks. Treatments were: high sugar (HS), an experimental perennial ryegrass variety bred to contain high concentrations of WSC; or control, a standard variety of perennial ryegrass (cv. AberElan) containing typical concentrations of WSC. The two grass varieties were matched in terms of heading date. All animals also received 4 kg day–1 standard dairy concentrate. Grass dry matter (DM) intake was not significantly different between treatments (11·6 vs. 10·7 kg DM day–1; s.e.d. 0·95 for HS and control diets respectively), although DM digestibility was higher on the HS diet (0·71 vs. 0·64 g g–1 DM; s.e.d. 0·23; P 〈 0·01) leading to higher digestible DM intakes for that diet. Milk yield from animals offered the HS diet was higher (15·3 vs. 12·6 kg day–1; s.e.d. 0·87; P 〈 0·05) and, although milk constituent concentrations were unaffected by treatment, milk protein yields were significantly increased on the HS diet. The partitioning of feed N was significantly affected by diet, with more N from the HS diet being used for milk production (0·30 vs. 0·23 g milk N g–1 feed N; s.e.d. 0·012; P 〈 0·01) and less being excreted in urine (0·25 vs. 0·35; s.e.d. 0·020; P 〈 0·01). In a separate experiment, using the same grasses harvested earlier in the season, the fractional rate of DM degradation, measured by in situ and gas production techniques, was higher for the HS grass than for the control. It is concluded that increased digestible DM intakes of the HS grass led to increased milk yields, whereas increased efficiency of utilization of the HS grass in the rumen resulted in the more efficient use of feed N for milk production and reduced N excretion.
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Grass and forage science 59 (2004), S. 0 
    ISSN: 1365-2494
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
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  • 4
    ISSN: 1365-2494
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The implications for the agricultural productivity of the UK upland sheep systems of reducing nitrogen fertilizer application and lowering stocking rates on perennial ryegrass/white clover swards were studied over 4 years at a site in Wales. The system involved grazing ewes and lambs from birth to weaning on swards maintained at a constant height with surplus herbage made into silage, thereafter ewes and weaned lambs grazed on separate areas until the onset of winter with adjustments to the size of the areas grazed and utilizing surplus pasture areas for silage. Four stocking rates [SR 18, 15, 12 and 9 ewes ha−1 on the total area (grazed and ensiled)] and two levels of annual nitrogen fertilizer application (N 200 and 50 kg ha−1) were studied in five treatments (N200/SR18, N200/SR15, N50/SR15, N50/SR12 and N50/SR9). Average white clover content was negatively correlated with the level of annual nitrogen fertilizer application. White clover content of the swards was maintained over the duration of the experiment with an increasing proportion of clover in the swards receiving 50 kg N ha−1. Control of sward height and the contribution from white clover resulted in similar levels of lamb liveweight gain from birth to weaning in all treatments but fewer lambs reached the slaughter live weight by September at the higher stocking rates and with the lower level of fertilizer application. Three of the five treatments provided adequate winter fodder as silage (N200/SR15, N50/SR12 and N50/SR9). Because of the failure to make adequate winter fodder and the failure of white clover to fully compensate for reduction in nitrogen fertilizer application, it is concluded that nitrogen fertilizer can only be reduced on upland sheep pastures if accompanied by reduced stocking rates.
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford UK : Blackwell Science Ltd
    Grass and forage science 56 (2001), S. 0 
    ISSN: 1365-2494
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: An experiment was established in 1991 on a 25-year-old perennial ryegrass/bent (Lolium perenne L./Agrostiscapillaris L.)-dominated pasture in Wales to study the effects of reducing nutrient inputs to previously fertilized upland pasture. The effects of the removal of applications of (1) N (denoted by CaPK) (2) N, P and K (Ca) and (3) N, P, K, and Ca (Nil) were compared with a treatment which received applications of all four nutrients (CaPKN) over a 6-year period (1991–96) in a randomized block design replicated three times. The experiment was managed under a continuous variable stocking regime (ewes and lambs until weaning and ewes thereafter) maintaining a sward surface height of 4·0 cm throughout the grazing season. Although individual liveweight gain of the lambs was unaffected by the treatments, there was a significant reduction (P 〈 0·05) in total lamb liveweight gain, ewe stocking rate and length of grazing season as a result of the withdrawal of nutrients. Over the 6 years total lamb liveweight gain was reduced by 17%, 32% and 45% and ewe stocking rate by 21%, 36% and 49% on treatments CaPK, Ca and Nil, respectively, compared with treatment CaPKN. The effect of withdrawing nutrient inputs on ewe stocking rate was progressive and by 1996 the Nil input treatment displayed a 63% reduction compared with the CaPKN treatment and this was also coupled with a 21-day reduction in length of the grazing season. During the post-weaning period, ewes from the Nil input treatment recorded a liveweight loss in 1995 and only a modest liveweight gain in 1996. This coupled with significantly lower body condition scores (P 〈 0·01) of these ewes in the autumn indicated that the Nil input treatment could lead to reductions in reproductive performance.
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  • 6
    ISSN: 1365-2494
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Using new harvesting and ensiling technologies, it is now possible to ensile kale (Brassica oleracea) successfully. However, there is little information available regarding the optimum time for harvest. The aim of this 2-year study was to compare the yield, fermentation characteristics and feeding value of kale harvested at different stages of growth, and ensiled with and without a bacterial inoculant. During Year 1 the crop was harvested after 15, 18 and 20 weeks of growth. The yield at each harvest was similar, but as the crop matured the crude protein (CP) concentration and buffering capacity decreased significantly, and there was a marked increase in the water-soluble carbohydrate (WSC) concentration. During Year 2 the crop was harvested at 14 and 17 weeks of growth. Again, the stage of maturity did not affect yield and, on this occasion, the chemical composition of the crop was unaffected by harvest date. However, the WSC and CP concentrations of the crop were lower in Year 2 than in Year 1, possibly as a result of differences in fertilizer regime. Each of the silages produced in Year 2 was offered to six Suffolk cross wether lambs, aged 10 months, to measure voluntary intake, in vivo digestibility and nitrogen retention. Neither harvesting date nor the use of an inoculant affected voluntary intake or nitrogen retention by the lambs. However, in vivo digestibility was higher in the kale silage harvested after 14 weeks of growth and when an inoculant was applied. The results obtained suggest that harvesting kale after 14 weeks of growth can produce highly digestible silage with a high CP concentration. Although delaying harvest until 18 weeks of growth will probably result in a decrease in the CP concentration of the crop, it should also lead to an increase in the WSC concentration of the crop, ensuring a more reliable fermentation.
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Plant pathology 49 (2000), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Phytoplasmas detected by fluorescence microscopy and polymerase chain reaction (PCR) have been discovered infecting Prunus trees at a site in south-east England. The pathogens were detected in tissue samples taken in autumn and also in spring. The symptoms in infected trees varied from severe decline to absence. PCR experiments using group-specific primers to amplify regions of the 16S RNA gene indicated that the phytoplasmas are similar to European stone fruit yellows isolates occurring in southern and eastern Europe. This is the first record of phytoplasmas in Prunus species in the UK. The origin of the infection is unknown. The implications of this new disease for the fruit industry are discussed.
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  • 8
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 75 (1906), S. 102-102 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] IN your issue of March 24, 1904, Mr. Nagaoka gives an account of a lecture experiment on magnetostriction; a few weeks later Prof. W. S. Franklin describes an experiment of the same kind. Both experimenters use a vertical solenoid, along the axis of which is fixed at its upper extremity an iron ...
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  • 9
    ISSN: 1546-1718
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] Inherited defects of base excision repair have not been associated with any human genetic disorder, although mutations of the genes mutM and mutY, which function in Escherichia coli base excision repair, lead to increased transversions of G:C to T:A. We have studied family N, which is affected with ...
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  • 10
    ISSN: 1432-1939
    Keywords: Key words Phosphorus ; Parasitic angiosperms ; Host-parasite interactions ; Sink strength ; Rhinanthus minor (yellow rattle)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The effects of phosphorus supply on the outcome of interactions between the hemiparasitic angiosperm Rhinanthus minor L. with its host species Lolium perenne L. were investigated in a glasshouse experiment. Host plants were grown in 3-l pots in the presence and absence of R. minor at limiting (0.13 mm P) and optimal (0.65 mm P) concentrations of phosphorus for the growth of the host species. Phosphorus was supplied at 2-day intervals in the form of half-strength Long Ashton nitrate-based solution with phosphorus concentrations adjusted accordingly. Parasitism by R. minor significantly suppressed host growth, with final biomass losses ranging between 32% and 44%. Phosphorus supply had a marked impact on the outcome of the host-parasite interaction. By the end of the growing period, parasite biomass at 0.65 mm P was 90% lower than that achieved at 0.13 mm P. In contrast, host biomass at 0.65 mm P was 74% higher than achieved at 0.13 mm P, indicting that the negative impact of parasitism on the host species was reduced when phosphorus supply was increased. The effects of phosphorus on the host-parasite association appeared to be mediated by changes in both the morphological characteristics of the host roots and the relative sink strengths of the host and parasite.
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