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  • 1
    Electronic Resource
    Electronic Resource
    Fabrication of large periodic arrays of AlGaAs microdisks by laser-interference lithography and selective etching (2002)
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 81 (2002), S. 592-594 
    Details
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: By laser-interference lithography, reactive-ion etching, and selective wet-chemical etching using a citric acid-based solution, we have fabricated large periodic arrays of AlGaAs microdisks with periods of 4 μm and disk diameters between 1.5 and 2 μm. The arrays are characterized by temperature-dependent photoluminescence spectroscopy. Taking into account the below-threshold absorption of the quantum wells inside the disks, we get disk quality factors close to the theoretical maximum value. We demonstrate that our technique allows one also to produce one-dimensionally or two-dimensionally coupled arrays of microdisks. © 2002 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1495082
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  • 2
    Electronic Resource
    Electronic Resource
    High resolution temperature and density profiles during the energy quench of density limit disruptions in Rijnhuizen tokamak project (2002)
    [S.l.] : American Institute of Physics (AIP)
    Physics of Plasmas 9 (2002), S. 3402-3412 
    Details
    ISSN: 1089-7674
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Measurements of the electron temperature, Te, and density, ne, during the energy quench of a major disruption showed that the onset of Te erosion in the neighborhood of the m/n=2/1 O point at the low field side (LFS) accelerates the well-known m/n=1/1 erosion of the core temperature. During this phase Te(r) is only partially flat in the region between the q=2 and the q=1 surfaces and ne(r) decreases in the core and increases inside the m/n=2/1 island. Immediately after the flattening of Te(r) a large peak in Te and to a lesser extent in ne has been observed. This peak is radially localized at the q=2 radius at the LFS, is very short lived and is poloidally asymmetric. Te profiles measured by the heterodyne radiometer and the Thomson scattering agree very well up to the time Te(r) flattens but afterwards can be a factor of two different. © 2002 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1489675
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  • 3
    Electronic Resource
    Electronic Resource
    The nuclear actin-related protein Act3p/Arp4p of Saccharomyces cerevisiae is involved in transcription regulation of stress genes (2003)
    Oxford, UK : Blackwell Publishing Ltd.
    Molecular microbiology 50 (2003), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: A mutational analysis of the essential nuclear actin-related protein of Saccharomyces cerevisiae, Act3p/Arp4p, was performed. The five residues chosen for substitution were amino acids conserved between actin and Act3p/Arp4p, the tertiary structure of which most probably resembles that of actin. Two thermosensitive (ts) mutants, a single and a double point mutant, and one lethal double point mutant were obtained. Both ts mutants were formamide-sensitive which supports a structural relatedness of Act3p/Arp4p to actin; they were also hypersensitive against hydroxyurea and ultraviolet irradiation pointing to a possible role of Act3p/Arp4p in DNA replication and repair. Their ‘suppressor of Ty’ (SPT) phenotype, ob-served with another ts mutant of Act3p/Arp4p before, suggested involvement of Act3p/Arp4p in transcription regulation. Accordingly, genome-wide expression profiling revealed misregulated transcription in a ts mutant of a number of genes, among which increased expression of various stress-responsive genes (many of them requiring Msn2p/Msn4p for induction) was the most salient result. This provides an explanation for the mutant's enhanced resistance to severe thermal and oxidative stress. Thus, Act3p/Arp4p takes an important part in the repression of stress-induced genes under non-stress conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2958.2003.03759.x
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  • 4
    Electronic Resource
    Electronic Resource
    A common drug-responsive element mediates the upregulation of the Candida albicans ABC transporters CDR1 and CDR2, two genes involved in antifungal drug resistance (2002)
    Oxford, UK : Blackwell Science Ltd.
    Molecular microbiology 43 (2002), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Upregulation of the ATP-binding cassette (ABC) transporter genes CDR1 and CDR2 (Candidadrug resistance 1 and 2) is a common mechanism observed in Candida albicans clinical isolates developing resistance to the class of azole antifungals. In this work, the regulatory elements of both genes were delimited using a reporter system in an azole-susceptible strain exposed to oestradiol, which allows transient induction of these genes. We found two regulatory elements in the CDR1 promoter: one responsible for basal expression (basal expression element; BEE) and the other required for oestradiol responsiveness (drug-responsive element I; DREI). In the CDR2 promoter, a single regulatory element responsible for oestradiol responsiveness (DREII) was detected. Both DREs shared a consensus of 21 bp with the sequence 5′-CGGA(A/T)ATCGGATATTTTTTTT-3′ having no equivalent to known eukaryotic regulatory sequence. Consistent with this finding, two other C. albicans genes identified by a search for the presence of DRE in the C. albicans genome sequence database were responsive to oestradiol. Finally, the regulatory elements found in CDR1 and CDR2 were also functional in an azole-resistant strain with constitutive high expression of both transporters. These results suggest that, although CDR1 and CDR2 upregulation can be obtained by transient drug-induced and constitutive upregulation, these two processes converge to the same regulatory elements and probably mobilize the same trans-acting factors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2958.2002.02814.x
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  • 5
    Electronic Resource
    Electronic Resource
    TFIIB and subunits of the SAGA complex are involved in transcriptional activation of phospholipid biosynthetic genes by the regulatory protein Ino2 in the yeast Saccharomyces cerevisiae (2003)
    Oxford, UK : Blackwell Science Ltd
    Molecular microbiology 48 (2003), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: In the yeast Saccharomyces cerevisiae, genes involved in phospholipid biosynthesis are activated by ICRE (inositol/choline-responsive element) up-stream motifs and the corresponding heterodimeric binding factor, Ino2 + Ino4. Both Ino2 and Ino4 contain basic helix–loop–helix (bHLH) domains required for ICRE binding, whereas transcriptional activation is mediated exclusively by Ino2. In this work, we describe a molecular analysis of functional minimal domains responsible for specific DNA recognition and transcriptional activation (TAD1 and TAD2). We also define the importance of individual amino acids within the more important activation domain TAD1. Random mutagenesis at five amino acid positions showed the importance of acidic as well as hydrophobic residues within this minimal TAD. We also investigated the contribution of known general transcription factors and co-activators for Ino2-dependent gene activation. Although an ada5 single mutant and a gal11 paf1 double mutant were severely affected, a partial reduction in activation was found for gcn5 and srb2. Ino2 interacts physically with the basal transcription factor Sua7 (TFIIB of yeast). Interestingly, interaction is mediated by the HLH dimerization domain of Ino2 and by two non-overlapping domains within Sua7. Thus, Sua7 may compete with Ino4 for binding to the Ino2 activator, creating the possibility of positive and negative influence of Sua7 on ICRE-dependent gene expression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2958.2003.03501.x
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  • 6
    Electronic Resource
    Electronic Resource
    The negative regulator Opi1 of phospholipid biosynthesis in yeast contacts the pleiotropic repressor Sin3 and the transcriptional activator Ino2 (2001)
    Oxford, UK : Blackwell Science Ltd
    Molecular microbiology 41 (2001), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Structural genes of phospholipid biosynthesis in the yeast Saccharomyces cerevisiae are transcriptionally co-regulated by ICRE (inositol/choline-responsive element) promoter motifs. Gene activation by an ICRE is mediated by binding of the Ino2/Ino4 transcription factor, whereas repression in the presence of high concentrations of inositol and choline (IC) requires an intact Opi1 repressor. However, the mechanism of specific repression and the functional interplay among these regulators remained unclear from previous work. Using in vivo as well as in vitro interaction assays, we show binding of the pleiotropic repressor Sin3 to the pathway-specific regulator Opi1. The paired amphipathic helix 1 (PAH1) within Sin3 and OSID (Opi1–Sin3 interaction domain) in the N-terminus of Opi1 were mapped as contact sites. The regulatory significance of the Opi1–Sin3 interaction was shown by the obvious deregulation of an ICRE-dependent reporter gene in a sin3 mutant. Opi1 also interacts with a newly identified functional domain of the transcriptional activator Ino2 (RID, repressor interaction domain). These results define the molecular composition of the transcription complex mediating control of ICRE-dependent genes and allow a hypothesis on the flow of regulatory information in response to phospholipid precursors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2958.2001.02495.x
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  • 7
    Electronic Resource
    Electronic Resource
    Die Diorite von Dessau (1952)
    Springer
    Contributions to mineralogy and petrology 3 (1952), S. 77-96 
    Details
    ISSN: 1432-0967
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Zusammenfassung Petrographische und chemische Untersuchungen an Dioriten und Graniten des Kyffhäuser und von Dessau lassen eine Ichor-Metamorphose, d. h. eine Alkali-Metasomatose von den Porenräumen aus, erkennen. In den metamorph-metablastischen Granitabkömmlingen wird die Ichor-Metamorphose deutlich an einer Glomeroblastenbildung und einer erneuten körnigen Texturentwicklung. Diese beweist eine Zufuhr von Albitmolekül. Sie wird in dem metamorph-metablastischen dioritischen Gestein neben der Verdrängung der primären Plagioklase aus der Alkalisierung und Enteisenung der Hornblende ersichtlich. Die magmatischen Ausgangsgesteine: Diorit, Quarzdiorit, Biotitdiorit, Gabbrodiorit (?) und Hornblendegabbro zeigen neben der Alkalisierung eine Muskovitisierung der basischen Plagioklase und eine Biotitisierung der Hornblende, woraus eine Zufuhr an Kali hervorgeht. Diese Stadien der Metamorphose durchlaufen den tektonischen Raum von der Grenze metamorph-nichtmetamorph bis hinab zur Grenze metamorph-magmatisch (Erdmannsdörffer) und rind in der Mitteldeutschen Schwelle zuerst aus dem tiefsten Stockwerk, im Südwesten im Odenwald-Spessart (Erdmannsdörffer) und nunmehr auch aus ihrer äußersten Haube erkannt worden, wo die Schwelle unter die Phyllithülle hinabtaucht.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01130079
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  • 8
    Electronic Resource
    Electronic Resource
    Über einen ungewöhnlichen Saponit von Raschau i. Sa. (1950)
    Springer
    Contributions to mineralogy and petrology 2 (1950), S. 279-290 
    Details
    ISSN: 1432-0967
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01098088
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  • 9
    Electronic Resource
    Electronic Resource
    Über den Nachweis von echtem Leverrierit in Tonsteinen aus unterkarbonischen Steinkohlenflözen von Dobrilugk (1950)
    Springer
    Contributions to mineralogy and petrology 2 (1950), S. 269-278 
    Details
    ISSN: 1432-0967
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Zusammenfassung Die Existenz des Leverrierit als selbständiges Mineral wird mineralogisch (A. Schüller) und chemisch (H. Grassmann) nachgewiesen. Zusammenfassend seien die Eigenschaften des Leverrierit und des mit ihm verwechselten Kaolinit gegenübergestellt (Tabelle 4).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01098087
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  • 10
    Electronic Resource
    Electronic Resource
    Die Tonsteine aus den Steinkohlenflözen von Dobrilugk und ihre Entstehung (1951)
    Springer
    Contributions to mineralogy and petrology 2 (1951), S. 413-427 
    Details
    ISSN: 1432-0967
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Zusammenfassung Es werden Tonsteine beschrieben, die als „biochemische” Sedimente im Steinkohlenmoor gebildet wurden. Die bisherige Deutung, solche Tonsteine seien ehemalige vulkanische Aschentuffe, wird als zur Zeit petrographisch unbegründet abgelehnt.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01083630
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