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  • 1
    Electronic Resource
    Electronic Resource
    Sulphydryl groups in intrinsic polypeptides of Micrococcus lysodeikticus membranes: Their role in protein transconformation during membrane solubilization (1979)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 6 (1979), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1979.tb03711.x
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  • 2
    Electronic Resource
    Electronic Resource
    Identification of the ornithine decarboxylase gene in the putrescine-producer Oenococcus oeni BIFI-83 (2004)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 239 (2004), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: We report here the identification of an ornithine decarboxylase (ODC) gene in the putrescine-producer Oenococcus oeni BIFI-83 strain. The gene contains a 2,235-nucleotide open reading frame encoding a 745-amino acid residues protein with a deduced molecular mass of 81 kDa. The primary structure of the ODC deduced from the nucleotide sequence has a consensus sequence containing the pyridoxal-5-phosphate (PLP) binding domain, and the critical amino acids residues involved in enzymatic activity are also conserved. As determined by BLAST analysis, the deduced amino acid sequence of the odc gene shares a 67% identity with the ODC protein from Lactobacillus 30a. The odc gene appears to be rarely present in the genome of O. oeni, since in a screening for the presence of this gene in 42 oenococcal strains none of the strains possessed an odc gene copy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/j.femsle.2004.08.036
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  • 3
    Electronic Resource
    Electronic Resource
    The pathogen Mycobacterium marinum, a faster growing close relative of Mycobacterium tuberculosis, has a single rRNA operon per genome (2004)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 235 (2004), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Although Mycobacterium marinum and Mycobacterium tuberculosis are very closely related they differ significantly in their growth rates. The Type strain of M. marinum and one clinical isolate were investigated and, like M. tuberculosis, were found to have a single rRNA (rrn) operon per genome located downstream from murA gene and controlled by two promoters. No sequence differences were found that account for the difference in the growth rates of the two species. We infer that M. tuberculosis has the capacity to synthesize rRNA much faster than it actually does; and propose that the high number of insertion sequences in this species attenuate growth rate to lower values.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.2004.tb09600.x
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  • 4
    Electronic Resource
    Electronic Resource
    The ovarian morphology of Scorpaena notata shows a specialized mode of oviparity (2002)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 61 (2002), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Scorpaena notata is an oviparous species with external fertilization that deposits its eggs in a gelatinous matrix. The internal epithelium of the ovarian wall is chiefly responsible for the production of this matrix, which is particularly abundant and viscous during the spawning period. The oocytes lack lipid droplets, so flotation and transport of the eggs is probably accomplished by means of the matrix that surrounds them. The ovarian stroma is situated along the centre of the gonad and the developing oocytes are connected to it by peduncles. The paucity and small size of the cortical alveoli of the oocytes are notable, as is the thinness of the zona radiata. These are characteristics that would be typical of viviparous species. The histological and ultrastructural observations lead to the conclusion that this species presents a type of oviparity more highly specialized than that of the majority of teleosts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1095-8649.2002.tb01849.x
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  • 5
    Electronic Resource
    Electronic Resource
    Ontogenetic changes in the diet of the herbivorous Scartichthys viridis in a rocky intertidal zone in central Chile (2000)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 56 (2000), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Scartichthys viridis maintains a herbivorous diet following recruitment to rocky intertidal areas, where it consumes almost exclusively macroalgae. The sheet–like green macroalgae Ulva and Enteromorpha were the main items consumed by individuals 〈130 mm LT. The tough branching red macroalga Gelidium made the bulk of the gut contents of specimens 〉220 mm LT, Ulva being consumed to a much lesser extent. Further, Gelidium increased in importance in the total gut contents during ontogeny. In contrast, both small (70–120 mm LT) and medium–sized (140–210 mm LT) S. viridis individuals preferred Ulva in the laboratory. It is suggested that the increasing consumption of Gelidium along the ontogeny of S. viridis results from the limited availability of Ulva in the field. Large S. viridis individuals possessed longer guts relative to their body length, in comparison with small individuals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1095-8649.2000.tb00887.x
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  • 6
    Electronic Resource
    Electronic Resource
    Gene transfer using human papillomavirus pseudovirions varies according to virus genotype and requires cell surface heparan sulfate (2001)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 204 (2001), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Artificial viruses consisting of DNA plasmid packaged in vitro into virus-like particles (VLPs) are new vehicles for gene transfer. We therefore investigated the ability of nine human papillomavirus (HPV) VLPs to interact with heterologous DNA and transfer genes. HPV 16, 18, 31, 33, 39, 45, 58, 59, and 68 VLPs were able to bind heterologous DNA and to transfer genes into Cos-7 cells. Inhibition of gene transfer by preincubation of the pseudovirions with heparin confirmed that heparan sulfate on the cell surface plays a role as cell receptor for HPVs. As HPV neutralizing antibodies are mainly type-specific, gene transfer with different HPV pseudovirions offers the possibility of their sequential use in vivo for a greater efficacy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.2001.tb10883.x
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  • 7
    Electronic Resource
    Electronic Resource
    Comparative study on the selective chalcopyrite bioleaching of a molybdenite concentrate with mesophilic and thermophilic bacteria (2001)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 196 (2001), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: This study evaluates different bioleaching treatments of a molybdenite concentrate using mesophilic and thermophilic bacterial cultures. Further studies on the chemical leaching and the electrochemical behavior of the MoS2 concentrate were carried out. Bioleaching tests showed a progressive removal of chalcopyrite from the molybdenite concentrate with an increase in temperature. Chemical leaching tests support the idea of an indirect attack of the concentrate. Electrochemical tests indicate that chalcopyrite dissolution is favored when molybdenite is present. Therefore, this type of bioleaching treatment could be applied to purify molybdenite flotation concentrates by selectively dissolving chalcopyrite.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.2001.tb10543.x
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  • 8
    Electronic Resource
    Electronic Resource
    Mitigation of N2O emissions from grassland by nitrification inhibitor and Actilith F2 applied with fertilizer and cattle slurry (2002)
    Oxford, UK : Blackwell Publishing Ltd
    Soil use and management 18 (2002), S. 0 
    Details
    ISSN: 1475-2743
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract. Nitrous oxide (N2O) is involved in both ozone destruction and global warming. In agricultural soils it is produced by nitrification and denitrification mainly after fertilization. Nitrification inhibitors have been proposed as one of the management tools for the reduction of the potential hazards of fertilizer-derived N2O. Addition of nitrification inhibitors to fertilizers maintains soil N in ammonium form, thereby gaseous N losses by nitrification and denitrification are less likely to occur and there is increased N utilization by the sward. We present a study aimed to evaluate the effectiveness of the nitrification inhibitor dicyandiamide (DCD) and of the slurry additive Actilith F2 on N2O emissions following application of calcium ammonium nitrate or cattle slurry to a mixed clover/ryegrass sward in the Basque Country. The results indicate that large differences in N2O emission occur depending on fertilizer type and the presence or absence of a nitrification inhibitor. There is considerable scope for immediate reduction of emissions by applying DCD with calcium ammonium nitrate or cattle slurry. DCD, applied at 25 kg ha–1, reduced the amount of N lost as N2O by 60% and 42% when applied with cattle slurry and calcium ammonium nitrate, respectively. Actilith F2 did not reduce N2O emissions and it produced a long lasting mineralization of previously immobilized added N.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1475-2743.2002.tb00231.x
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  • 9
    Electronic Resource
    Electronic Resource
    Impact of pig slurry on soil properties, water salinization, nitrate leaching and crop yield in a four-year experiment in Central Spain (2004)
    Oxford, UK : Blackwell Publishing Ltd
    Soil use and management 20 (2004), S. 0 
    Details
    ISSN: 1475-2743
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract. The repeated application of pig slurry to agricultural soils may result in an accumulation of salts and a risk of aquifer pollution due to nitrate leaching and salinization. Under Mediterranean conditions, a field experiment on a sandy loam soil (Typic Xerofluvent) was performed with maize (Zea mays) in 1998, 1999 and 2001 to study the effects of applying optimal (P1) and excessive rates (P3) of pig slurry on soil salinization, nitrate leaching and groundwater pollution. The rate of pig slurry was established considering the optimal N rate for maize in this soil (170, 162 and 176 kg N ha−1 for 1998, 1999 and 2001, respectively). Pig slurry treatments were compared to an optimal N rate supplied as urea (U) and a control treatment without N fertilizer (P0). The composition of the slurries showed great variability between years. Mean NO3− leaching losses from 1998 to 2001 were 329, 215, 173 and 78 kg N ha−1 for P3, P1, U and P0 treatments, respectively. The amount of total dissolved salts (TDS) added to the soil in slurry application between 1998 and 2001 was 2019 kg TDS ha−1 for the P1 treatment and 6058 kg TDS ha−1 for the P3 treatment. As a consequence, the electrical conductivity (EC) of the slurry-treated soils was greater than that of the control soil. The EC correlated significantly with the sodium concentration of the soil solution. Over the entire experimental period, 2653, 2202 and 2110 kg Na ha−1 entered the aquifer from the P3, P1 and P0 treatments, respectively. The P3 treatment did not significantly increase grain production in 1999 and 2001 compared with that achieved with the optimal N rate treatment (P1). This behaviour shows the importance of establishing application guidelines for pig slurry that will reduce the risk of soil and groundwater pollution.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1475-2743.2004.tb00395.x
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  • 10
    Electronic Resource
    Electronic Resource
    Direct Visualization of the Microtubular Cytoskeleton of Ciliated Protozoa with a Fluorescent Taxoid (2002)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 49 (2002), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: . Visualization of the infraciliature, which is an essential tool for the identification of ciliate species, has traditionally been obtained with silver proteinate methods. Since infraciliature is mainly composed of microtubules, we used the synthetic fluorescent taxoid FLUTAX as a method for ciliate identification. The main advantages of this method are the facility and rapidity of its application and the fact that no previous fixation and permeabilization processes are required. FLUTAX may also be used as a probe to follow morphogenetical changes in the microtubular cytoskeleton during the ciliate life cycle.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.2002.tb00376.x
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