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  • 2005-2009  (82)
  • 2000-2004  (46)
  • 1995-1999  (60)
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Current genetics 28 (1995), S. 225-234 
    ISSN: 1432-0983
    Keywords: Pythium ; Linear mitochondrial DNA ; Pulsed-field gel electrophoresis ; ATPase9 ; mtDNA replication
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Pulsed-field gel electrophoresis (PFGE) of isolates of Pythium oligandrum with linear mitochondrial genomes revealed a distinct band in ethidium bromide-stained gels similar in size to values estimated by restriction mapping of mitochondrial DNA (mtDNA). Southern analysis confirmed that these bands were mtDNA and indicated that linear genomes were present in unit-length size as well as multimers. Isolates of this species with circular mtDNA restriction maps also had low levels of linear mono- and multimers. visualized by Southern analysis of PFGE gels. Examination of 17 additional species revealed similar results; three species had distinct linear mtDNA bands in ethidium bromide-stained gels while the remainder had linear mono- and multi-mers in lower amounts detected only by Southern analysis. Sequence analysis of an isolate of P. oligandrum with a primarily circular mitochondrial genomic map and a low amount of linear molecules revealed that the small unique region of the circular map (which corresponded to the terminal region of linear genomes) was flanked by palindromic intrastrand complementary sequences separated by a unique 194-bp sequence. Sequences with similarity to ATPase9 coding regions from other organisms were located adjacent to this region. Sequences with similarity to mitochondrial origins of replication and autonomously replicating sequences were also located in this region: their potential involvement in the generation of linear molecules is discussed.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 114 (2001), S. 3988-3993 
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: We report the observation of a new c-type band of (D2O)3 at 583.215 92(37) GHz, which we assign to the k=±20←±10 torsional hot-band. The new data includes the first observation of K=0 states for the k=+10 and k=−20 levels and effects a correct assignment of these states. A new perturbation was observed for the K=2 states of the k=+20←−10 subband splitting each transition into two equally spaced equal intensity doublets. Analysis of the band and inclusion into a global fit of all torsional bands produces negligible differences with previous analyses, and confirms the validity of the Hamiltonian developed to treat the coupling between torsional motion and overall rotation. The 583.2 GHz band completes the precise characterization of all (D2O)3 vibrational levels below 100 cm−1. © 2001 American Institute of Physics.
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  • 3
    Electronic Resource
    Electronic Resource
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 114 (2001), S. 3994-4004 
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: We report the first direct observation of the hydrogen-bond stretching vibration for a water cluster. A perpendicular band of (D2O)3 was measured by terahertz laser vibration–rotation–tunneling spectroscopy at 142.8 cm−1 in the "translational band" region of the liquid corresponding to the hindered translational motions of water molecules. We have tentatively assigned the spectrum to transitions from the vibrational ground state to the degenerate hydrogen-bond stretch or a combination or mixed state of the degenerate stretch and a torsional vibration. Comparison with theoretical results shows that calculated frequencies are much too high, presumably because they do not include coupling between the torsional and stretching vibrations. © 2001 American Institute of Physics.
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  • 4
    Electronic Resource
    Electronic Resource
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 114 (2001), S. 4005-4015 
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: We report the first high resolution spectrum of a librational vibration for a water cluster. Four parallel bands of (H2O)3 were measured between 510 and 525 cm−1 using diode laser vibration–rotation–tunneling (VRT) spectroscopy. The bands lie in the "librational band" region of liquid water and are assigned to the nondegenerate out of plane librational vibration. The observation of at least three distinct bands within 8 cm−1 originating in the vibrational ground state is explained by a dramatically increased splitting of the rovibrational levels relative to the ground state by bifurcation tunneling and is indicative of a greatly reduced barrier height in the excited state. This tunneling motion is of special significance, as it is the lowest energy pathway for breaking and reforming of hydrogen bonds, a salient aspect of liquid water dynamics. © 2001 American Institute of Physics.
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  • 5
    Electronic Resource
    Electronic Resource
    Bingley : Emerald
    Kybernetes 34 (2005), S. 343-352 
    ISSN: 0368-492X
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Computer Science
    Notes: Purpose - The purpose of this paper is to highlight the influence of the late Heinz von Foerster in the life of an admirer and friend who remains a leader in the field of family therapy. Design/methodology/approach - The approach is autobiographical. The method is (loosely) process recall and personal theoretical reflection. Objectives are achieved by organizing major tenets of von Foerster's constructivism into personal categories of meaning. Findings - Findings included a deep philosophical influence on the author's professional life; an abiding impact on his personal life; and a durability of von Foerster's ideas across disciplines, time, and the ever-changing theories and politics of the mental health profession. Originality/value - This paper adds value to the social sciences in general, and to the field of family therapy in particular, because it highlights the interconnectedness of person and profession and the confluence of the messenger/message. Some might say this is cybernetics in vivo. There may be little that is "new" in this paper in terms of theoretical constructs, but the autobiographical nature of the reflections may be its most valuable contribution to others struggling with such concepts.
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  • 6
    Electronic Resource
    Electronic Resource
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 111 (1999), S. 7801-7806 
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: Vibration–rotation–tunneling (VRT) spectroscopy has been extended to the 4 THz spectral region through the observation of a second intermolecular vibration of (D2O)4. Analysis of the precisely measured perpendicular transition confirms the previously reported cyclic homodromic structure and reveals a dramatically increased (30×) hydrogen bond rearrangement rate in the excited state. © 1999 American Institute of Physics.
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  • 7
    Electronic Resource
    Electronic Resource
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Phytopathology 41 (2003), S. 325-350 
    ISSN: 0066-4286
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Biology
    Notes: The current standard treatment for management of soilborne pests in some high-value crop production systems is preplant fumigation with mixtures of methyl bromide and chloropicrin. With the impending phase-out of methyl bromide, the agricultural industries that rely on soil fumigation face the need for development of alternative pest management strategies. To maintain farm productivity, immediate term research has focused on evaluation of alternative fumigants, modification of current crop production practices to accommodate their use, and improvement of application technologies to reduce the environmental effects of fumigant applications. Longer-term research goals have focused on developing a more integrated approach for pest management that incorporates the use of cultural practices to reduce pathogen pressure, host resistance to disease, and biological approaches for stimulating plant growth and control of root diseases.
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  • 8
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 21 (1996), S. 0 
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract: The possibilities of mobilizing recombinant DNA (rDNA) by strains from activated sludge have been studied. A mixture of ten bacterial strains isolated from activated sludge was grown on a support in a continuously fed fixed-bed reactor or in suspension in a sequenced-batch reactor with biomass recycling. After reaching steady state, the bacteria in both types of reactors were used as the recipient population for evaluating the dissemination of the non-conjugative and non-mobilizable recombinant plasmid pCE328. The dissemination of this plasmid, derived from pBR328 (tra−, mob−, oriT−), was studied in comparison with that of pCE325 (pBR325 derivative tra−, mob−, oriT+), and that of the natural broad host range conjugative plasmid R388 (IncW). Initially the mobilization properties of pBR type recombinant plasmids between two strains of Escherichia coli were determined in the fixed-bed reactor. Only the mobilization of the recombinant plasmid bearing a transfer origin (pCE325) was observed. The survival of E. coli strains bearing either the non-transferable plasmid pCE328 or the conjugative plasmid R388 and added to the activated sludge reactors was measured in the effluent of both types of reactors. In the fixed-bed reactor effluent, the donor populations remained stable after three days of operation and reached 103 to 105 colony forming units (cfu) ml−1, whereas they decreased in the sequenced-batch reactors: donors bearing the pCE328 plasmid decreased by 2 log units in 20 days, whereas those bearing the R388 plasmid decreased by less than 1 log unit. Mobilization of the pCE328 plasmid could not be detected in any case. Transfer of the conjugative plasmid R388, used as a positive propagation control, was detected in strains arising from activated sludge in both types of reactors. Nevertheless, establishment of transconjugants was observed only in the fixed biomass at the rate of 10−7 transconjugants per recipient.
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  • 9
    ISSN: 1520-4804
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1520-4804
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
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