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  • 1
    Electronic Resource
    Electronic Resource
    Electroporation of cells (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 79 (1990), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: By measuring uptake of the membrane impermeable dye. phenosafranine, it can be shown that the plasma membrane of intact cells within cell aggregates can be reversibly permeabilized by electroporation. However, the plant cell wall is a barrier to DNA uptake by intact cells, although under certain circumstances expression of DNA, electroporated into intact cells, can be demonstrated. The level of expression is about 20–50 times lower than that obtained by electroporation of protoplasts, and depends on cell wall properties and pretreatments of cell aggregates. In contrast, efficient transformation of whole cells of bacteria and yeasts can be achieved by electroporation. Factors which influence DNA transfer into whole plant cells and the possibility of stable transformation are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1990.tb05881.x
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  • 2
    Electronic Resource
    Electronic Resource
    Transgenic yellow lupin (Lupinus luteus) (2000)
    Springer
    Plant cell reports 19 (2000), S. 634-637 
    Details
    ISSN: 1432-203X
    Keywords: Key words Lupinus luteus ; Yellow lupin ; Agrobacterium tumefaciens ; bar gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Transgenic yellow lupin (Lupinus luteus L.) plants have been generated by meristem co-cultivation with Agrobacterium tumefaciens. The binary plasmid pPZBNIa contains the bar gene under the control of a CaMV 35 S promoter. The transformation method involves inoculation of embryonic axis explants with A. tumefaciens, flooding the meristem with glufosinate, and initial culture on non-selective medium. Shoots were transferred to culture medium containing 20 mg/l glufosinate. Following subculture, shoots were grafted onto non-transgenic narrow-leafed lupin (L. angustifolius L.) seedling rootstocks, or rooted in vitro. The overall transformation efficiency, as determined at the T1 generation, was 0.05%–0.75%. The transgenic nature of plants grown to the T6 generation was confirmed by phosphinothricin acetyl transferase, PCR and Southern analyses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050785
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  • 3
    Electronic Resource
    Electronic Resource
    Plant regeneration from protoplasts of Solanum species with potential agricultural value (S. hjertingii, S. polyadenium, S. capsicibaccatum) (1991)
    Springer
    Plant cell reports 9 (1991), S. 520-522 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Protoplasts have been isolated from three tuber-bearing Solanum species, S. hjertingii, S. polyadenium and S. capsicibaccatum, that are sexually incompatible with S. tuberosum, but possess potentially useful characters. For isolating protoplasts from leaves of in vitro shoot cultures of S. hjertingii and S. capsicibaccatum growth was improved by including silver thiosulfate in the medium. However, for S. polyadenium, leaves of pot-grown plants were the best source for protoplasts. Following protoplast division and culture, plants were regenerated from protoplasts of each of the species. The pattern of chromosome variation in regenerants was similar to that observed for other diploid and tetraploid Solanum species. The results indicate that it should be possible to introduce the potentially useful germplasm from these wild species into somatic hybrids with S. tuberosum by protoplast fusion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232110
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  • 4
    Electronic Resource
    Electronic Resource
    Species-specific sequences in the genus Solanum: identification, characterization, and application to study somatic hybrids of S. brevidens and S. tuberosum (1990)
    Springer
    Theoretical and applied genetics 80 (1990), S. 693-698 
    Details
    ISSN: 1432-2242
    Keywords: Potato ; Solanum brevidens ; Somatic hybrids ; Species-specific probes ; Squash blots
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary To aid in the identification and analysis of somatic hybrids between potato (Solanum tuberosum, dihaploid line PDH 40) and the non tuber-bearing wild species S. brevidens, a series of species-specific repetitive DNA sequences have been isolated. This was accomplished by making libraries of HaeIII-digested total DNA of S. tuberosum and S. brevidens, by cloning fragments into the SmaI site of plasmid pUC18 and transforming them into E. coli (JM83). The S. brevidens library consisted of 1,000 recombinant clones, and that of S. tuberosum, 700. Nitrocellulose filters with recombinant clones were hybridised to nick-translated total DNA of S. brevidens and also S. tuberosum, and, following autoradiography, clones that hybridised strongly to the DNA of only one of the species were chosen. Two highly repeated S. brevidens clones (pSB1, 400 bp and pSB7,210 bp), one highcopy-number s. tuberosum clone (pST10, 200 bp) and one low-copy-number sequence of S. tuberosum (pST3, 1.5 kbp) were selected for further analysis by Southern hybridisation to digested total DNA. Clone pSB7 gave a ladder pattern on hybridisation to EcoR1-digested total DNA of S. brevidens, with signals at multiples of 200 bp DNA. Using these probes it was possible to verify the hybridity of putative hybrids of dihaploid S. tuberosum and S.brevidens, and to confirm by Southern analysis and by slot blots the parental genome dosage of hexaploid hybrids (two s. brevidens: one S. tuberosum, and vice-versa). The S. tuberosum-specific probe, pSTIO, hybridised with DNA of three other tuber-bearing wild species (S. hjertingii, S. capsicibaccatum and S. berthaultii). A squash-blot procedure was developed using the probes that would allow early identification of somatic hybrid callus. There are a number of useful applications of such species-specific probes in the identification and analysis of somatic hybrids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224231
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  • 5
    Electronic Resource
    Electronic Resource
    Analysis of the mitochondrial DNA of the somatic hybrids of Solanum brevidens and S. tuberosum using non-radioactive digoxigenin-labelled DNA probes (1993)
    Springer
    Theoretical and applied genetics 85 (1993), S. 1017-1022 
    Details
    ISSN: 1432-2242
    Keywords: Potato ; S. brevidens ; Mitchondrial DNA ; Somatic hybrids ; Non-radioactive labelling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mitochondrial (mt) DNAs of somatic hybrids obtained by electrical and chemical fusion of mesophyll protoplasts of S. brevidens and a dihaploid line of S. tuberosum PDH 40 were analysed by Southern hybridization using the digoxigenin-labelled mtDNA sequences nad5 or orf25. In the Southern analysis of the hybrid mtDNA probed with nad5, most of the 19 hybrids analyzed had an RFLP pattern similar, but not identical, to one of the parents, S. tuberosum, PDH40. Nineteen percent of the hybrids had most of the S. brevidens fragments. Five of the hybrids had an identical RFLP pattern to either one of the parents while another two hybrids had novel RFLP patterns. Similar results were obtained by Southern analysis with orf25. These results clearly show that mtDNA rearrangements had occurred at a high frequency in the somatic hybrids. There were no differences in the frequencies of rearrangements observed between the hybrids regenerated from chemical and electrical fusions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00215042
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  • 6
    Electronic Resource
    Electronic Resource
    Production of asymmetric hybrids between Solanum tuberosum and irradiated S. brevidens (1993)
    Springer
    Theoretical and applied genetics 85 (1993), S. 729-734 
    Details
    ISSN: 1432-2242
    Keywords: Protoplast fusion ; Gamma irradiation ; Partial genome transfer ; Solanum tuberosum ; Solanum brevidens
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Asymmetric somatic hybrids were obtained by fusion of Solanum tuberosum (PDH40) protoplasts with 300- or 500-Gy irradiated protoplasts of S. brevidens. These radiation doses were sufficient to prevent the growth of the S. brevidens protoplasts. Putative hybrids were selected on the basis of phenotype from regenerated shoots and identified with a S. brevidens-specific probe. From these, 31 asymmetric hybrids were confirmed by morphological characteristics, isoenzyme patterns and RFLP analysis. The morphology of the asymmetric hybrids was intermediate between that of S. tuberosum and symmetric hybrids of both species (obtained without irradiation treatment). Chromosome counts from 17 asymmetric hybrids showed that the chromosome number of the hybrids ranged from 31 to 64. The asymmetric hybrids probably had one or two genome complements (i.e. either 24 or 48 chromosomes) from S. tuberosum and 7–22 chromosomes from S. brevidens. There was no clear correlation between the radiation dose and the degree of elimination of the S. brevidens genome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225012
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  • 7
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    A Method for Optimizing Non-Axisymmetric Liners for Multimodal Sound Sources (2002)
    In:  CASI
    Details
    Publication Date: 2019-07-13
    Description: Central processor unit times and memory requirements for a commonly used solver are compared to that of a state-of-the-art, parallel, sparse solver. The sparse solver is then used in conjunction with three constrained optimization methodologies to assess the relative merits of non-axisymmetric versus axisymmetric liner concepts for improving liner acoustic suppression. This assessment is performed with a multimodal noise source (with equal mode amplitudes and phases) in a finite-length rectangular duct without flow. The sparse solver is found to reduce memory requirements by a factor of five and central processing time by a factor of eleven when compared with the commonly used solver. Results show that the optimum impedance of the uniform liner is dominated by the least attenuated mode, whose attenuation is maximized by the Cremer optimum impedance. An optimized, four-segmented liner with impedance segments in a checkerboard arrangement is found to be inferior to an optimized spanwise segmented liner. This optimized spanwise segmented liner is shown to attenuate substantially more sound than the optimized uniform liner and tends to be more effective at the higher frequencies. The most important result of this study is the discovery that when optimized, a spanwise segmented liner with two segments gives attenuations equal to or substantially greater than an optimized axially segmented liner with the same number of segments.
    Keywords: Acoustics
    Type: AIAA Paper 2002-2516 , 8th AIAA/CEAS Aeroacoustics Conference; Jun 17, 2002 - Jun 19, 2002; Breckenridge, CO; United States
    Format: application/pdf
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    NASA TECHNICAL REPORTS
  • 8
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    Comparison of Acoustic Impedance Eduction Techniques for Locally-Reacting Liners (2003)
    In:  CASI
    Details
    Publication Date: 2019-07-13
    Description: Typical acoustic liners used in current aircraft inlets and aft-fan ducts consist of some type of perforated facesheet bonded to a honeycomb core. A number of techniques for determining the acoustic impedance of these locallyreacting liners have been developed over the last five decades. In addition, a number of models have been developed to predict the acoustic impedance of locallyreacting liners in the presence of grazing flow, and to use that information together with aeroacoustic propagation codes to assess the noise absorption provided by these liners. These prediction models have incorporated the results from databases acquired with specific impedance eduction techniques. Thus, while these prediction models are acceptable for liners that are similar to those tested in these databases, their application to new liner configurations must be viewed with caution. The primary purpose of this paper is to provide a comparison of impedance eduction techniques that have been implemented at various aerospace research laboratories in the United States (NASA Langley Research Center, General Electric Aircraft Engines, B. F. Goodrich and Boeing). A secondary purpose is to provide data for liner configurations that extend the porosity range beyond that which has been previously used in common aircraft engine nacelles. Two sets of liners were designed to study the effects of three parameters: perforate hole diameter, facesheet thickness and porosity. These two sets of liners were constructed for testing in each of the laboratories listed above. The first set of liners was designed to fit into the NASA Langley and Boeing test facilities. The second set was designed to fit into the General Electric Aircraft Engines and B. F. Goodrich test facilities. By using the same parent material, both sets of liners were identical to within the limits of material and fabrication variability. Baseline data were obtained in the normal incidence impedance tubes at NASA Langley and B. F. Goodrich. The results were found to compare extremely well. The samples were then tested in the grazing flow ducts of each of the four laboratories. Perhaps the most significant result of these comparisons is that the educed acoustic resistances for the liners used in this study increase as the mean flow profile is modified from uniform to 3-D shear. This realization has demonstrated the need for an frequency-dependent impedance eduction technique that incorporates 3-D shear flow and is efficient.
    Keywords: Acoustics
    Type: AIAA Paper 2003-3306 , 9th AIAA/CEAS Aeroacoustics Conference and Exhibit; May 12, 2003 - May 14, 2003; Hilton Head, SC; United States
    Format: application/pdf
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    NASA TECHNICAL REPORTS
  • 9
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    A Computational and Experimental Study of Slit Resonators (2003)
    In:  CASI
    Details
    Publication Date: 2019-07-13
    Description: Computational and experimental studies are carried out to offer validation of the results obtained from direct numerical simulation (DNS) of the flow and acoustic fields of slit resonators. The test cases include slits with 90-degree corners and slits with 45-degree bevel angle housed inside an acoustic impedance tube. Three slit widths are used. Six frequencies from 0.5 to 3.0 kHz are chosen. Good agreement is found between computed and measured reflection factors. In addition, incident sound waves having white noise spectrum and a prescribed pseudo-random noise spectrum are used in subsequent series of tests. The computed broadband results are again found to agree well with experimental data. It is believed the present results provide strong support that DNS can eventually be a useful and accurate prediction tool for liner aeroacoustics. The usage of DNS as a design tool is discussed and illustrated by a simple example.
    Keywords: Acoustics
    Type: AIAA Paper 2003-3310 , 9th AIAA/CEAS Aeroacoustics Conference and Exhibit; May 12, 2003 - May 14, 2003; Hilton Head, SC; United States
    Format: application/pdf
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  • 10
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    MeerKAT-64 discovers wide-spread tidal debris in the nearby NGC 7232 galaxy group (2021)
    Oxford University Press
    Details
    Publication Date: 2021-06-02
    Description: We report the discovery of large amounts of previously undetected cold neutral atomic hydrogen (H i) around the core triplet galaxies in the nearby NGC 7232 galaxy group with MeerKAT. With a physical resolution of ∼1 kpc, we detect a complex web of low-surface-brightness H i emission down to a 4σ column density level of ∼1 × 1019 cm−2 (over 44  km s−1). The newly discovered H i streams extend over ∼20 arcmin corresponding to 140 kpc in projection. This is approximately three times the H i extent of the galaxy triplet (52 kpc). The H i debris has an H i mass of ∼6.6 × 109 M⊙, more than 50 per cent of the total H i mass of the triplet. Within the galaxy triplet, NGC 7233 and NGC 7232 have lost a significant amount of H i while NGC 7232B appears to have an excess of H i. The H i deficiency in NGC 7232 and NGC 7233 indicates that galaxy–galaxy interaction in the group concentrates on this galaxy pair while the other disc galaxies have visited them over time. In comparison to the AMIGA sample of isolated galaxies, we find that with regards to its total H i mass the NGC 7232/3 galaxy triplet is not H i-deficient. Despite the many interactions associated to the triplet galaxies, no H i seems to have been lost from the group (yet).
    Print ISSN: 0035-8711
    Electronic ISSN: 1365-2966
    Topics: Physics
    Published by Oxford University Press on behalf of The Royal Astronomical Society.
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