Publication Date:
2022-05-26
Description:
Author Posting. © The Authors, 2005. This is the author's version of the work. It is posted here by permission of American Association for the Advancement of Science for personal use, not for redistribution. The definitive version was published in Science 311 (2006): 388-391, doi:10.1126/science.1122142.
Description:
The stable propagation of genetic material during cell division depends on the congression
of chromosomes to the spindle equator before the cell initiates anaphase. It is generally assumed
that congression requires that chromosomes are connected to the opposite poles of the bipolar
spindle (i.e., “bi-oriented”). We found that chromosomes can congress before becoming bioriented.
By combining the use of reversible chemical inhibitors, live-cell light microscopy and
correlative electron microscopy, we found that mono-oriented chromosomes could glide towards
the spindle equator alongside kinetochore fibers attached to other already bi-oriented
chromosomes. This congression mechanism depended on the kinetochore-associated plus enddirected
microtubule motor CENP-E (kinesin-7).
Description:
Supported by grants from
the NIH (GM59363 to A.K., GM65933 to T.M.K., GM24364 to E.D.S, and GM06627 to
B.F.M)
Repository Name:
Woods Hole Open Access Server
Type:
Preprint
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