Publication Date:
2009-03-03
Description:
Influenza virus presents an important and persistent threat to public health worldwide, and current vaccines provide immunity to viral isolates similar to the vaccine strain. High-affinity antibodies against a conserved epitope could provide immunity to the diverse influenza subtypes and protection against future pandemic viruses. Cocrystal structures were determined at 2.2 and 2.7 angstrom resolutions for broadly neutralizing human antibody CR6261 Fab in complexes with the major surface antigen (hemagglutinin, HA) from viruses responsible for the 1918 H1N1 influenza pandemic and a recent lethal case of H5N1 avian influenza. In contrast to other structurally characterized influenza antibodies, CR6261 recognizes a highly conserved helical region in the membrane-proximal stem of HA1 and HA2. The antibody neutralizes the virus by blocking conformational rearrangements associated with membrane fusion. The CR6261 epitope identified here should accelerate the design and implementation of improved vaccines that can elicit CR6261-like antibodies, as well as antibody-based therapies for the treatment of influenza.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2758658/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2758658/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Ekiert, Damian C -- Bhabha, Gira -- Elsliger, Marc-Andre -- Friesen, Robert H E -- Jongeneelen, Mandy -- Throsby, Mark -- Goudsmit, Jaap -- Wilson, Ian A -- AI-058113/AI/NIAID NIH HHS/ -- P01 AI058113/AI/NIAID NIH HHS/ -- P01 AI058113-040002/AI/NIAID NIH HHS/ -- U54 GM074898/GM/NIGMS NIH HHS/ -- U54 GM074898-03/GM/NIGMS NIH HHS/ -- Y1-CO-1020/CO/NCI NIH HHS/ -- Y1-GM-1104/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2009 Apr 10;324(5924):246-51. doi: 10.1126/science.1171491. Epub 2009 Feb 26.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Molecular Biology, Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19251591" target="_blank"〉PubMed〈/a〉
Keywords:
Antibodies, Viral/chemistry/*immunology
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*Antibody Affinity
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Antigens, Viral/chemistry/*immunology
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*Binding Sites, Antibody
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Crystallization
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Crystallography, X-Ray
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Epitopes/immunology
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Glycosylation
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Hemagglutinin Glycoproteins, Influenza Virus/chemistry/*immunology
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Humans
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Hydrogen Bonding
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Hydrogen-Ion Concentration
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Hydrophobic and Hydrophilic Interactions
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Immunoglobulin Fab Fragments/chemistry/*immunology
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Influenza A Virus, H1N1 Subtype/*immunology
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Influenza A Virus, H5N1 Subtype/*immunology
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Influenza Vaccines
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Membrane Fusion
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Models, Molecular
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Neutralization Tests
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Protein Conformation
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Protein Structure, Secondary
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Protein Structure, Tertiary
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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