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  • 1
    Publication Date: 2022-06-09
    Description: Exploration of the Martian subsurface, to depths from a few metres to many kilometres, offers an unprecedented opportunity to answer one of the biggest questions contemplated by humankind: was or is there life beyond Earth? Simultaneously, Mars subsurface exploration lays the foundation for self-sufficient human settlements beyond our own planet and provides an emerging potential for synergistic collaborations with the rising commercial space sector and traditional mining companies. Our understanding of the Martian subsurface and the technologies for exploring it — with a dual focus on the search for signs of extinct and extant life, and resource characterization and acquisition — have matured enough for serious consideration as part of future robotic missions to Mars.
    Description: Published
    Description: 116–120
    Description: 6A. Geochimica per l'ambiente
    Description: JCR Journal
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
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  • 2
    Publication Date: 2016-11-07
    Description: Many cells can sense and respond to time-varying stimuli, selectively triggering changes in cell fate only in response to inputs of a particular duration or frequency. A common motif in dynamically controlled cells is a dual-timescale regulatory network: although long-term fate decisions are ultimately controlled by a slow-timescale switch (e.g., gene expression), input signals are first processed by a fast-timescale signaling layer, which is hypothesized to filter what dynamic information is efficiently relayed downstream. Directly testing the design principles of how dual-timescale circuits control dynamic sensing, however, has been challenging, because most synthetic biology methods have focused solely on rewiring transcriptional circuits, which operate at a single slow timescale. Here, we report the development of a modular approach for flexibly engineering phosphorylation circuits using designed phospho-regulon motifs. By then linking rapid phospho-feedback with slower downstream transcription-based bistable switches, we can construct synthetic dual-timescale circuits in yeast in which the triggering dynamics and the end-state properties of the ON state can be selectively tuned. These phospho-regulon tools thus open up the possibility to engineer cells with customized dynamical control.
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
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  • 3
    Publication Date: 2016-07-09
    Description: We present a case study of energetic ions observed by the Energetic Particle Detector (EPD) on the Magnetospheric Multiscale (MMS) spacecraft in the magnetosheath just outside the subsolar magnetopause that occurred at 1000 UT on December 8, 2015. As the magnetopause receded inward, the EPD observed a burst of energetic (50−5000 keV) proton, helium, and oxygen ions that exhibited an inverse dispersion, with the lowest energy ions appearing first. The prolonged interval of fast antisunward flow observed in the magnetosheath and transient increases in the H components of global ground magnetograms demonstrate that the burst appeared at a time when the magnetosphere was rapidly compressed. We attribute the inverse energy dispersion to the leakage along reconnected magnetic field lines of betatron-accelerated energetic ions in the magnetosheath and a burst of reconnection has an extent of about 1.5 R E using combined Super Dual Auroral Radar Network (SuperDARN) radar and EPD observations.
    Print ISSN: 0094-8276
    Electronic ISSN: 1944-8007
    Topics: Geosciences , Physics
    Published by Wiley on behalf of American Geophysical Union (AGU).
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  • 4
    Publication Date: 2016-07-16
    Description: The Cloud Imaging and Particle Size (CIPS) instrument on the Aeronomy of Ice in the Mesosphere (AIM) satellite provides an opportunity to study the longitudinal variation in polar mesospheric cloud (PMC). We examined the longitudinal variation in PMC albedo using 8 years (2007-2014) of observations from the CIPS instrument. The results show that the PMC albedo in the Southern Hemisphere (SH), especially in the latitude band of 80°S-85°S, is persistently low (~65% relative to the rest of the hemisphere) within 60°W to 150°W longitude. In the Northern Hemisphere (NH), however, PMC albedo is found to be relatively zonally asymmetry. Harmonic analyses shows that the persistent longitudinal variation in the SH PMC albedo is due to zonal wavenumbers 1 through 4 (WN1-WN4) processes with minima in the longitude range of 60°W-150°W. The influence of temperature and H 2 O on the longitudinal variation of the PMC albedo is discussed based on results obtained using a simple 0-D PMC model and temperature from the Microwave Limb Sounder (MLS) and the Sounding of the Atmosphere with Broadband Emission Radiometry (SABER) and H 2 O from MLS. The modeled region of low ice mass in the SH is generally consistent with that of low PMC albedo seen in CIPS. Tidal analyses using the SABER temperatures indicate that the non-migrating semidiurnal tides with modes of S0, W1 and E1 might be the main drivers of the persistent longitudinal variations of PMC albedo in the SH. Non-migrating tides are much weaker in the NH and consistent with the observed lack of longitudinal variability in PMC albedo.
    Print ISSN: 0148-0227
    Topics: Geosciences , Physics
    Published by Wiley on behalf of American Geophysical Union (AGU).
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  • 5
    Publication Date: 2015-05-16
    Description: While the most important feature of magnetic reconnection is that it energizes plasma particles by converting magnetic energy to particle energy, the exact mechanisms by which this happens are yet to be determined despite a long history of reconnection research. Recently, we have reported our results on the energy conversion and partitioning in a laboratory reconnection layer in a short communication [Yamada et al. , Nat. Commun. 5 , 4474 (2014)]. The present paper is a detailed elaboration of this report together with an additional dataset with different boundary sizes. Our experimental study of the reconnection layer is carried out in the two-fluid physics regime where ions and electrons move quite differently. We have observed that the conversion of magnetic energy occurs across a region significantly larger than the narrow electron diffusion region. A saddle shaped electrostatic potential profile exists in the reconnection plane, and ions are accelerated by the resulting electric field at the separatrices. These accelerated ions are then thermalized by re-magnetization in the downstream region. A quantitative inventory of the converted energy is presented in a reconnection layer with a well-defined, variable boundary. We have also carried out a systematic study of the effects of boundary conditions on the energy inventory. This study concludes that about 50% of the inflowing magnetic energy is converted to particle energy, 2/3 of which is ultimately transferred to ions and 1/3 to electrons. Assisted by another set of magnetic reconnection experiment data and numerical simulations with different sizes of monitoring box, it is also observed that the observed features of energy conversion and partitioning do not depend on the size of monitoring boundary across the range of sizes tested from 1.5 to 4 ion skin depths.
    Print ISSN: 1070-664X
    Electronic ISSN: 1089-7674
    Topics: Physics
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  • 6
    Publication Date: 2016-08-17
    Description: Journal of Proteome Research DOI: 10.1021/acs.jproteome.6b00331
    Print ISSN: 1535-3893
    Electronic ISSN: 1535-3907
    Topics: Chemistry and Pharmacology
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  • 7
    Publication Date: 2015-07-15
    Description: A morphological study revealed that the NW American soft-bottom bivalve Nutricola tantilla is dioecious, not a protandric hermaphrodite as previously reported. We base this conclusion on the smaller males having highly specialized, glandular sperm ducts, while the larger females have simple oviducts and no transition between the two ducts occurs. The females are brooders and retain their ova in a marsupium within the dorsal part of the inner demibranchs. Sperm cells were present and associated with a mesh-like tissue among the ova or early-stage embryos. How this tissue originates is still unknown. We suggest that sperm cells dissociate from this pool and fertilize the oocytes as soon as they are ovulated. The mode of sperm storage in N. tantilla represents a unique case in that the sperm are presumably nurtured within a nonepithelial tissue. We describe the ultrastructure of the sperm cells in N. tantilla. Large, spherical cytophores become associated with a multitude of acrosomes of spermatozoa and probably represent the precursors of the spermatozeugmata that have been described previously.
    Print ISSN: 0260-1230
    Electronic ISSN: 1464-3766
    Topics: Biology
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  • 8
    Publication Date: 2015-10-06
    Description: ABSTRACT The large amount video data produced by multi-channel, high-resolution microscopy system drives the need for a new high-performance domain-specific video compression technique. We describe a novel compression method for video microscopy data. The method is based on Pearson's correlation and mathematical morphology. The method makes use of the point-spread function (PSF) in the microscopy video acquisition phase. We compare our method to other lossless compression methods and to lossy JPEG, JPEG2000, and H.264 compression for various kinds of video microscopy data including fluorescence video and brightfield video. We find that for certain data sets, the new method compresses much better than lossless compression with no impact on analysis results. It achieved a best compressed size of 0.77% of the original size, 25× smaller than the best lossless technique (which yields 20% for the same video). The compressed size scales with the video's scientific data content. Further testing showed that existing lossy algorithms greatly impacted data analysis at similar compression sizes. Microsc. Res. Tech., 2015 . © 2015 Wiley Periodicals, Inc.
    Print ISSN: 1059-910X
    Electronic ISSN: 1097-0029
    Topics: Natural Sciences in General
    Published by Wiley
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  • 9
    Publication Date: 2016-02-23
    Description: Adaptation services are the ecosystem processes and services that benefit people by increasing their ability to adapt to change. Benefits may accrue from existing but newly-used services where ecosystems persist, or from novel services supplied following ecosystem transformation. Ecosystem properties that enable persistence or transformation are important adaptation services because they support future options. The adaptation services approach can be applied to decisions on trade-offs between currently valued services and benefits from maintaining future options. For example, ecosystem functions and services of floodplains depend on river flows. In those regions of the world where climate change projections are for hotter, drier conditions, floods will be less frequent and floodplains will either persist, though with modified structure and function, or transform to terrestrial (flood-independent) ecosystems. Many currently valued ecosystem services will reduce in supply or become unavailable, but new options are provided by adaptation services. We present a case study from the Murray-Darling Basin, Australia, for operationalizing the adaptation services concept for floodplains and wetlands. We found large changes in flow and flood regimes are likely under a scenario of +1.6°C by 2030, even with additional water restored to rivers under the proposed Murray-Darling Basin Plan. We predict major changes to floodplain ecosystems, including contraction of riparian forests and woodlands and expansion of terrestrial, drought-tolerant vegetation communities. Examples of adaptation services under this scenario include substitution of irrigated agriculture with dryland cropping and floodplain grazing; mitigation of damage from rarer, extreme floods; and increased tourism, recreational and cultural values derived from fewer, smaller wetlands that can be maintained with environmental flows. Management for adaptation services will require decisions on where intervention can enable ecosystem persistence and where transformation is inevitable. New ways of managing water that includes consideration of the increasing importance of adaptation services requires major changes to decision-making that better account for landscape heterogeneity and large-scale change, rather than attempting to maintain ecosystems in fixed states. This article is protected by copyright. All rights reserved.
    Print ISSN: 1051-0761
    Electronic ISSN: 1939-5582
    Topics: Biology
    Published by Wiley on behalf of The Ecological Society of America (ESA).
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  • 10
    Publication Date: 2019
    Description: 〈p〉Tuberculosis, caused by the intracellular pathogen 〈i〉Mycobacterium tuberculosis〈/i〉, remains the world’s deadliest infectious disease. Sterilizing chemotherapy requires at least 6 months of multidrug therapy. Difficulty visualizing the subcellular localization of antibiotics in infected host cells means that it is unclear whether antibiotics penetrate all mycobacteria-containing compartments in the cell. Here, we combined correlated light, electron, and ion microscopy to image the distribution of bedaquiline in infected human macrophages at submicrometer resolution. Bedaquiline accumulated primarily in host cell lipid droplets, but heterogeneously in mycobacteria within a variety of intracellular compartments. Furthermore, lipid droplets did not sequester antibiotic but constituted a transferable reservoir that enhanced antibacterial efficacy. Thus, strong lipid binding facilitated drug trafficking by host organelles to an intracellular target during antimicrobial treatment.〈/p〉
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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