Publication Date:
2024-02-23
Description:
A process of global importance in carbon cycling is the remineralization of algae biomass by heterotrophic bacteria, most notably during massive marine algae blooms. Such blooms can trigger secondary blooms of planktonic bacteria that consist of swift successions of distinct bacterial clades, most prominently members of the Flavobacteriia, Gammaproteobacteria and the alphaproteobacterial Roseobacter clade. This study explores such successions during spring phytoplankton blooms in the southern North Sea (German Bight) for four consecutive years. The surface water samples were taken at Helgoland Island about 40 km offshore in the southeastern North Sea in the German Bight at the station 'Kabeltonne' (54° 11.3' N, 7° 54.0' E) between the main island and the minor island, Düne (German for 'dune') using small research vessels (http://www.awi.de/en/expedition/ships/more-ships.html). Water depths at this site fluctuate from 6 to 10 m over the tidal cycle. Samples were processed as described previously (Teeling et al., 2012; doi:10.7554/eLife.11888.001) in the laboratory of the Biological Station Helgoland within less than two hours after sampling. Assessment of absolute cell numbers and bacterioplankton community composition was carried out as described previously (Thiele et al., 2011; doi:10.1016/B978-0-444-53199-5.00056-7). To obtain total cell numbers, DNA of formaldehyde fixed cells filtered on 0.2 mm pore sized filters was stained with 4',6-diamidino-2-phenylindole (DAPI). Fluorescently labeled cells were subsequently counted on filter sections using an epifluores-cence microscope. Likewise, bacterioplankton community composition was assessed by catalyzedreporter deposition fluorescence in situ hybridization (CARD-FISH) of formaldehyde fixed cells on 0.2 mm pore sized filters.
Keywords:
Actinobacteria, targeted with HGC69a oligonucleotides FISH-probe; Alphaproteobacteria, targeted with ALF968 oligonucleotides FISH-probe; Alteromonas/Colwellia, targeted with Alt1413 oligonucleotide FISH-probe; Archaea, targed with ARCH915 oligonucleotide FISH-probe; Bacteria, targed with EUB338(I-III) oligonucleotide FISH-probe; Bacterioplankton; Balneatrix, targeted with Bal731 oligonucleotides FISH-probe; Betaproteobacteria, targeted with BET42a oligonucleotides FISH-probe; Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH); Crenarchaeota marine group I, targeted with Cren554 oligonucleotide FISH-probe; Cytophaga-Flavobacterium cluster, targeted with CF319a oligonucleotide FISH-probe; DATE/TIME; DEPTH, water; Epifluorescence microscopy after DAPI staining; Euryarchaeota marine group II, targeted with Eury806 oligonucleotide FISH-probe; Formosa, targeted with FORM181A oligonucleotides FISH-probe; Formosa, targeted with FORM181B oligonucleotides FISH-probe; Gammaproteobacteria, targeted with Gam42a oligonucleotide FISH-probe; German Bight, North Sea; Glaciecola, targeted with Glac227 oligonucleotides FISH-probe; HelgolandRoads_bacterioplankton_2009-2012; Julian day; Kabeltonne; Marinoscillum, targeted with CYT-734 oligonucleotides FISH-probe; NAC11-7 clade, targeted with NAC11-7-1030 oligonucleotides FISH-probe; Non-bacteria control, targeted with NON338 oligonucleotides FISH-probe; NOR5 clade, targeted with NOR5-730 oligonucleotides FISH-probe; NS3a clade, targeted with NS3a-840 oligonucleotides FISH-probe; NS5 clade, targeted with NS5/DE2-471 oligonucleotides FISH-probe; NS5 clade, targeted with NS5/VIS1-575 oligonucleotides FISH-probe; NS9 clade, targeted with NS9-664 oligonucleotides FISH-probe; OM182 clade, targeted with OM182-707 oligonucleotides FISH-probe; Pirellula group D, targeted with PirD1039 oligonucleotides FISH-probe; Planctomyce group A, targeted with PlaA1228 oligonucleotides FISH-probe; Planctomycete group B, targeted with uPlaB440 oligonucleotides FISH-probe; Planctomycetes, targeted with Pla46 oligonucleotide FISH-probe; Polaribacter, targeted with POL740 oligonucleotides FISH-probe; Pseudoalteromonas, targeted with PSA184 oligonucleotide FISH-probe; Reinekea, targeted with REI731 oligonucleotides FISH-probe; Roseobacter clade, targeted with RCA1000 oligonucleotides FISH-probe; Roseobacter clade, targeted with ROS537 oligonucleotides FISH-probe; SAR11 clade, targeted with SAR11-441 oligonucleotide FISH-probe; SAR11 clade, targeted with SAR11-486 oligonucleotides FISH-probe; SAR324 clade, targeted with SAR324-1412 oligonucleotide FISH-probe; SAR86 clade, targeted with SAR86-1245 oligonucleotide FISH-probe; SAR92 clade, targeted with SAR92-627 oligonucleotides FISH-probe; Ulvibacter, targeted with ULV995 oligonucleotides FISH-probe; Vibrio, targeted with GV841 oligonucleotide FISH-probe; VIS6 clade, targeted with VIS6-814 oligonucleotides FISH-probe
Type:
Dataset
Format:
text/tab-separated-values, 5239 data points
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