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  • 2015-2019  (1)
  • 1995-1999  (2)
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  • 1
    Electronic Resource
    Electronic Resource
    Osney Mead, Oxford OX2 0EL, UK : Blackwell Scientific Publications
    Molecular microbiology 17 (1995), S. 0 
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: A large chemotaxis operon was identified in Rhodobacter sphaeroides WS8-N using a probe based on the 3′ terminal portion of the Rhizobium meliloti cheA gene. Two genes homologous to the enteric cheY were identified in an operon also containing cheA, cheW, and cheR homologues. The deduced protein sequences of che gene products were aligned with those from Escherichia coli and shown to be highly conserved. A mutant with an interrupted copy of cheA showed normal patterns of swimming, unlike the equivalent mutants in E. coli which are smooth swimming. Tethered cheA mutant cells showed normal responses to changes in organic acids, but increased, inverted responses to sugars. The unusual behaviour of the cheA mutant and the identification of two homologues of cheY suggests that R. sphaeroides has at least two pathways controlling motor activity. To identify functional similarity between the newly identified R. sphaeroides Che pathway and the methyl-accepting chemotaxis protein (MCP)-dependent pathway in enteric bacteria, the R. sphaeroides cheW gene was expressed in a cheW mutant strain of E. coli and found to complement, causing a partial return to a swarming phenotype. In addition, expression of the R. sphaeroides gene in wild-type E. coli resulted in the same increased tumbling and reduced swarming as seen when the native gene is over-expressed in E. coli. The identification of che homologues in R. sphaeroides and complementation by cheW suggests the presence of MCPs in an organism previously considered to use only MCP-independent sensing. The MCP-dependent pathway, appears conserved. In R. sphaeroides this pathway may mediate responses to sugars, while responses to organic acids may in involve a second system, possibly using the second CheY protein identified in this study.
    Type of Medium: Electronic Resource
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  • 2
    Publication Date: 1997-06-02
    Print ISSN: 0014-5793
    Electronic ISSN: 1873-3468
    Topics: Biology , Chemistry and Pharmacology
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  • 3
    Publication Date: 2019-04-15
    Description: Corporate social responsibility (CSR) in the seafood industry is on the rise. Because of increasing public awareness and non-governmental organization (NGO) campaigns, seafood buyers have made various commitments to improve the sustainability of their wild seafood sourcing. As part of this effort, seafood suppliers have developed their own CSR programs in order to meet buyers’ sourcing requirements. However, the CSR of these companies, many of which are mid-supply chain or vertically integrated, remain largely invisible and unstudied. In order to better understand how mid-chain seafood suppliers engage in sustainability efforts, we reviewed the CSR practices of the 25 largest seafood companies globally (by revenue) that deal with wild seafood products. Based on literature, existing frameworks, and initial data analysis, we developed a structured framework to identify and categorize practices based on the issues addressed and the approach used. We found companies implement CSR to address four key areas, and through various activities that fit into five categories: Power; Practices; Partnerships; Public policy; and Philanthropy. One of the biggest gaps identified in this study is the lack of accountability mechanisms, as well as robust and consistent accounting of impacts. Indeed, many companies express commitments without clear goals and structures in place to ensure implementation. Therefore, improvements in seafood company performance on social and environmental aspects may not only require creating a better business case for CSR, but also require ensuring that companies have the necessary processes and structures in place through public oversights and regulations.
    Electronic ISSN: 2071-1050
    Topics: Energy, Environment Protection, Nuclear Power Engineering
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