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  • 2015-2019  (462)
  • 2018  (462)
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  • 2015-2019  (462)
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  • 1
    Call number: IASS 18.91837
    Type of Medium: Monograph available for loan
    Pages: x, 128 Seiten , Illustrationen
    ISBN: 9781509530564 , 9781509530571
    Uniform Title: Où atterir? Comment s'orienter en politique
    URL: Cover
    Language: English
    Branch Library: RIFS Library
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  • 2
    Publication Date: 2019-07-13
    Description: An inexpensive, laboratory-based, strain gauge valve gape monitor (SGM) was developed to monitor the valve gape behavior of bivalve molluscs in response to diel-cycling hypoxia. A Wheatstone bridge was connected to strain gauges that were attached to the shells of oysters (Crassostrea virginica). The recorded signals allowed for the opening and closing of the bivalves to be recorded continuously over two-day periods of experimentally-induced diel-cycling hypoxia and diel-cycling changes in pH. Here, we describe a protocol for developing an inexpensive strain gauge monitor and describe, in an example laboratory experiment, how we used it to measure the valve gape behavior of Eastern oysters (C. virginica), in response to diel-cycling hypoxia and cyclical changes in pH. Valve gape was measured on oysters subjected to cyclical severe hypoxic (0.6 mg/L) dissolved oxygen conditions with and without cyclical changes in pH, cyclical mild hypoxic (1.7 mg/L) conditions and normoxic (7.3 mg/L) conditions. We demonstrate that when oysters encounter repeated diel cycles, they rapidly close their shells in response to severe hypoxia and close with a time lag to mild hypoxia. When normoxia is restored, they rapidly open again. Oysters did not respond to cyclical pH conditions superimposed on diel cycling severe hypoxia. At reduced oxygen conditions, more than one third of the oysters closed simultaneously. We demonstrate that oysters respond to diel-cycling hypoxia, which must be considered when assessing the behavior of bivalves to dissolved oxygen. The valve SGM can be used to assess responses of bivalve molluscs to changes in dissolved oxygen or contaminants. Sealing techniques to better seal the valve gape strain gauges from sea water need further improvement to increase the longevity of the sensors.
    Keywords: Astrophysics
    Type: GSFC-E-DAA-TN54019 , Journal of Visualized Experiments (e-ISSN 1940-087X); 138
    Format: application/pdf
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  • 3
    Publication Date: 2022-05-25
    Description: Author Posting. © The Author(s), 2018. This is the author's version of the work. It is posted here under a nonexclusive, irrevocable, paid-up, worldwide license granted to WHOI. It is made available for personal use, not for redistribution. The definitive version was published in Ecosystem Services 31C (2018): 387-394, doi:10.1016/j.ecoser.2018.03.005.
    Description: The Hudson River and its estuary is once again an ecologically, economically, and culturally functional component of New York City’s natural environment. The estuary's cultural significance may derive largely from environmental education, including marine science programs for the public. These programs are understood as “cultural” ecosystem services but are rarely evaluated in economic terms. We estimated the economic value of the Hudson River Park’s environmental education programs. We compiled data on visits by schools and summer camps from 32 New York City school districts to the Park during the years 2014 and 2015. A “travel cost” approach was adapted from the field of environmental economics to estimate the value of education in this context. A small—but conservative—estimate of the Park’s annual education program benefits ranged between $7,500-$25,500, implying an average capitalized value on the order of $0.6 million. Importantly, organizations in districts with high proportions of minority students or English language learners were found to be more likely to participate in the Park’s programs. The results provide an optimistic view of the benefits of environmental education focused on urban estuaries, through which a growing understanding of ecological systems could lead to future environmental improvements.
    Description: We thank the Hudson River Foundation and Woods Hole Oceanographic Institution’s Summer Student Fellow program for financial support.
    Keywords: Ecosystem services ; Environmental education ; Hudson River ; Economic benefits ; Travel cost method ; Urban
    Repository Name: Woods Hole Open Access Server
    Type: Preprint
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  • 4
    Publication Date: 2022-05-26
    Description: Also published as: Journal of the Acoustical Society of America 68 (1980): 1750-1767
    Description: Acoustical signals at 270 Hz from SOFAR floats drifting in the region southeast of the Gulf Stream were recorded during most of 1975 from a near axis sound channel hydrophone near Bermuda. The amplitude levels received exhibit a large increase (12–18 dB) commencing about 24 July, following a long period (March to July) of relatively lower peak level amplitudes. A major part of the increase can be attributed to the influence of a large cyclonic eddy (Gulf Stream ring) that passed slowly between the SOFAR floats and Bermuda. Such an eddy produces a large sound speed anomaly that extends to depths below the axis of the sound channel. On 24 July, two SOFAR floats were known to have approximately the same sound transmission path through the edge of the large eddy. The sound transmission peaks occur when no ocean eddy is between the SOFAR floats and the receiver. Their spacing shows they occur at regular refraction caustics in the sound channel. When the sound transmission path passes through an eddy, these transmission focal distances are shifted to greater range and the signal level may be greatly enhanced. The decrease of caustic peak intensities with range is 5 dB per double distance, and this agrees with theory. Several different levels of peak acoustic intensity occur and these result from two float depths and oceanic thermocline oscillations.
    Description: Prepared for the Office of Naval Research under Contract N00014-74-C-0262; NR 083-004·.
    Keywords: Underwater acoustics ; Eddies
    Repository Name: Woods Hole Open Access Server
    Type: Technical Report
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  • 5
    Publication Date: 2022-05-26
    Description: Eighty-two CTD stations were taken in the Mediterranean Sea during February, 1975 . These stations were concentrated in the Alboran Sea near Gibraltar to investigate whether water typical of the deep western Mediterranean was flowing directly up and over the sill at Gibraltar. Temperature, salinity, and potential temperature at standard pressures are presented for each of the stations.
    Description: Prepared for the National Science Foundation under Grants OCE76-10940 and OCE74-19782 A03 and for the National Oceanographic Data Center under the National Oceanic and Atmospheric Administration Purchase Order 6-19641 to the Woods Hole Oceanographic Institution.
    Keywords: Oceanography ; Chain (Ship : 1958-) Cruise CH118
    Repository Name: Woods Hole Open Access Server
    Type: Technical Report
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  • 6
    Publication Date: 2022-05-26
    Description: Author Posting. © The Author(s), 2018. This is the author's version of the work. It is posted here under a nonexclusive, irrevocable, paid-up, worldwide license granted to WHOI. It is made available for personal use, not for redistribution. The definitive version was published in Marine Environmental Research 134 (2018): 96-108, doi:10.1016/j.marenvres.2018.01.002.
    Description: Estuarine organisms are subjected to combinations of anthropogenic and natural stressors, which together can reduce an organisms' ability to respond to either stress or can potentiate or synergize the cellular impacts for individual stressors. Nematostella vectensis (starlet sea anemone) is a useful model for investigating novel and evolutionarily conserved cellular and molecular responses to environmental stress. Using RNA-seq, we assessed global changes in gene expression in Nematostella in response to dispersant and/or sweet crude oil exposure alone or combined with ultraviolet radiation (UV). A total of 110 transcripts were differentially expressed by dispersant and/or crude oil exposure, primarily dominated by the down-regulation of 74 unique transcripts in the dispersant treatment. In contrast, UV exposure alone or combined with dispersant and/or oil resulted in the differential expression of 1133 transcripts, of which 436 were shared between all four treatment combinations. Most significant was the differential expression of 531 transcripts unique to one or more of the combined UV/chemical exposures. Main categories of genes affected by one or more of the treatments included enzymes involved in xenobiotic metabolism and transport, DNA repair enzymes, and general stress response genes conserved among vertebrates and invertebrates. However, the most interesting observation was the induction of several transcripts indicating de novo synthesis of mycosporine-like amino acids and other novel cellular antioxidants. Together, our data suggest that the toxicity of oil and/or dispersant and the complexity of the molecular response are significantly enhanced by UV exposure, which may co-occur for shallow water species like Nematostella.
    Description: This material is based upon work supported by the National Science Foundation under Grant No. MCB1057152 (MJJ), MCB1057354 (AMT) and DEB1545539 (AMR).
    Keywords: Hydrocarbons ; UV radiation ; Biomarker ; Chemical pollution ; Environmental toxicology ; RNA sequencing ; Cnidarian
    Repository Name: Woods Hole Open Access Server
    Type: Preprint
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  • 7
    Publication Date: 2022-05-26
    Description: Throughout the northeastern United States, aquaculture operators face a wide variety of laws and regulations that govern the manner in which they plan, site, and operate aquaculture facilities. Many local, state, and federal laws and regulations have been designed to enable aquaculture to exist as a viable industry and to flourish. It is obvious that aquaculture cannot be conducted in the absence of a legal system that establishes property rights, provides a means for the enforcement of these rights, and ensures the safety of the product for consumers. Although a legal framework is necessary for aquaculture to exist as an industry, there are many instances where uninformed, outdated, or inappropriate regulatory regimes impede aquaculture development (DoC 1999; MCZM 1995; Ewart et al. 1995; Rychlak and Peel 1993; Bye 1990; DeVoe and Mount 1989; Kennedy and Breisch 1983; NRC 1978). Inconsistencies in the law can lead to an uncertain legal environment for aquaculturists.1 Regulators are put in the conflicting position of promoting the development of the industry and regulating its effect on other uses of the land and sea (DeVoe 1999; NRC 1992). Operators are sometimes forced to undertake activities while lacking adequate information or a complete understanding of laws and regulations. Conflicts and concerns often may be left unresolved until an issue is brought before an adjudicatory body. Legal constraints such as these detract from the stability and certainty that otherwise would facilitate sustainable aquaculture development, slowing or halting the growth of the industry, or perhaps even leading to its decline. Such constraints make the statements quoted above as true today as they were 35 years ago. Policies that both facilitate and constrain aquaculture have been reviewed by a number of commentators (McCoy 2000; Brennan 1999; Barr 1997; Reiser and Bunsick 1999; Reiser 1997; Hopkins et al. 1997; Rychlak and Peel 1993; Eichenberg and Vestal 1992; Wildsmith 1982; Kane 1970). In 1981, the US Fish and Wildlife Service sponsored a comprehensive review of aquaculture regulation across the nation (the “Aspen Report”). The report’s authors identified at least 120 federal laws that, at that time, either directly (50 laws) or indirectly (70 laws) affected aquaculture. Further, the authors found more than 1,200 statutes regulating aquaculture in 32 states (ASC 1981). An important finding of the Aspen Report was that aquaculture businesses must obtain at least 30 permits, on average, in order to site and operate their businesses. McCoy (2000) concludes from his review of the Aspen Report and other studies that aquaculture may be the most highly regulated industry in America.2 In its responses to periodic surveys of constraining factors, the industry seems to agree with McCoy by consistently ranking legal and regulatory constraints near the top of the list of factors. Wypyszinszki et al. (1992) begin to assemble the body of law relating to marine aquaculture in the US Northeast, although their work remains unfinished due to insufficient resources. A number of excellent analyses emerged from that effort, including a study of the public trust doctrine by Eichenberg and Vestal (1992) and a study of “reverse regulation” of the oyster industry in Long Island Sound.3 Here we examine a range of aquaculture policies in an effort to identify those laws and regulations that may impede development unnecessarily within the northeastern United States. Through a survey of industry and government officials and a review of the literature, we find that specific laws and policies or the absence of laws and policies can be argued to impose constraints on growth in certain segments of the industry.
    Description: Funding was provided by the Northeastern Regional Aquaculture Center through Grant number 98-38500-5917 from the U.S. Department of Agriculture Cooperative State, Research, Education, and Extension Service (USDA-CSREES).
    Repository Name: Woods Hole Open Access Server
    Type: Working Paper
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  • 8
    Publication Date: 2018-08-20
    Description: Enterotoxigenic Escherichia coli (ETEC) is a global diarrheal pathogen that utilizes adhesins and secreted enterotoxins to cause disease in mammalian hosts. Decades of research on virulence factor regulation in ETEC has revealed a variety of environmental factors that influence gene expression, including bile, pH, bicarbonate, osmolarity, and glucose. However, other hallmarks of the intestinal tract, such as low oxygen availability, have not been examined. Further, determining how ETEC integrates these signals in the complex host environment is challenging. To address this, we characterized ETEC’s response to the human host using samples from a controlled human infection model. We found ETEC senses environmental oxygen to globally influence virulence factor expression via the oxygen-sensitive transcriptional regulator fumarate and nitrate reduction (FNR) regulator. In vitro anaerobic growth replicates the in vivo virulence factor expression profile, and deletion of fnr in ETEC strain H10407 results in a significant increase in expression of all classical virulence factors, including the colonization factor antigen I (CFA/I) adhesin operon and both heat-stable and heat-labile enterotoxins. These data depict a model of ETEC infection where FNR activity can globally influence virulence gene expression, and therefore proximity to the oxygenated zone bordering intestinal epithelial cells likely influences ETEC virulence gene expression in vivo. Outside of the host, ETEC biofilms are associated with seasonal ETEC epidemics, and we find FNR is a regulator of biofilm production. Together these data suggest FNR-dependent oxygen sensing in ETEC has implications for human infection inside and outside of the host.
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
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  • 9
    Publication Date: 2018-05-21
    Description: A central tenet of biology is that globular proteins have a unique 3D structure under physiological conditions. Recent work has challenged this notion by demonstrating that some proteins switch folds, a process that involves remodeling of secondary structure in response to a few mutations (evolved fold switchers) or cellular stimuli (extant fold switchers). To date, extant fold switchers have been viewed as rare byproducts of evolution, but their frequency has been neither quantified nor estimated. By systematically and exhaustively searching the Protein Data Bank (PDB), we found ∼100 extant fold-switching proteins. Furthermore, we gathered multiple lines of evidence suggesting that these proteins are widespread in nature. Based on these lines of evidence, we hypothesized that the frequency of extant fold-switching proteins may be underrepresented by the structures in the PDB. Thus, we sought to identify other putative extant fold switchers with only one solved conformation. To do this, we identified two characteristic features of our ∼100 extant fold-switching proteins, incorrect secondary structure predictions and likely independent folding cooperativity, and searched the PDB for other proteins with similar features. Reassuringly, this method identified dozens of other proteins in the literature with indication of a structural change but only one solved conformation in the PDB. Thus, we used it to estimate that 0.5–4% of PDB proteins switch folds. These results demonstrate that extant fold-switching proteins are likely more common than the PDB reflects, which has implications for cell biology, genomics, and human health.
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
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  • 10
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