Publikationsdatum:
2011-07-29
Beschreibung:
Laccase partially purified from residual compost of Agaricus bisporus by an aqueous two-phase system (Lac ATPS) was used in degrading polycyclic aromatic hydrocarbons: fluorene (Flu), phenanthrene (Phe), anthracene (Ant), benzo[a]pyrene (BaP), and benzo[a]anthracene (BaA). The capacity of the enzyme to oxidize polyaromatic compounds was compared to that of the crude laccase extract (CE). After treatment of 72 h, Lac ATPS and CE were not capable of oxidizing Flu and Phe, while Ant, BaP, and BaA were oxidized, resulting in percentages of oxidation of 11.2 ± 1, 26 ± 2, and 11.7 ± 4 % with CE, respectively. When Lac ATPS was used, the following percentages of oxidation were obtained: 11.4 ± 3 % for Ant, 34 ± 0.1 % for BaP, and 13.6 ± 2 % for BaA. The results reported here demonstrate the potential application of Lac ATPS for the oxidation of polycyclic aromatic hydrocarbons. Laccase partially purified from residual compost of A. bisporus by an aqueous two-phase system was used to degrade polycyclic aromatic hydrocarbons (PAHs). This work is the first to report on the use of laccase obtained through partial purification and used in the oxidation of PAHs.
Print ISSN:
0930-7516
Digitale ISSN:
1521-4125
Thema:
Chemie und Pharmazie
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Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
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