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  • 1
    Monograph available for loan
    Monograph available for loan
    Cambridge : Cambridge University Press
    Call number: PIK B 020-00-0361
    Type of Medium: Monograph available for loan
    Pages: 148 p.
    Edition: 1. ed.
    ISBN: 0521620082
    Location: A 18 - must be ordered
    Branch Library: PIK Library
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  • 2
    ISSN: 1573-3904
    Keywords: dipeptide analogues ; epimerization ; imidazolide-derived dipeptides ; keto esters
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary β-Keto esters derived from dipeptides are prepared by application of common methodologies employed for the synthesis of amino acid-derived β-keto esters; however, epimerization of the C-terminal residue occurred to different extents depending on the method. In imidazolide activated dipeptides, this epimerization is due to the CDI activation step and to the configurational instability of the intermediate imidazolides in different reaction media. Regarding yield and diastereomeric purity, the method of choice proved to be the reaction of dipeptide-derived imidazolide with the potassium salt of malonic half esters in the presence of MgCl2.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-3904
    Keywords: dipeptide analogues ; epimerization ; imidazolide-derived dipeptides ; keto esters
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract β-Keto esters derived from dipeptides areprepared by application of common methodologiesemployed for the synthesis of amino acid-derivedβ-keto esters; however, epimerization of theC-terminal residue occurred to different extentsdepending on the method. In imidazolide activateddipeptides, this epimerization is due to the CDIactivation step and to the configurational instabilityof the intermediate imidazolides in different reactionmedia. Regarding yield and diastereomeric purity, themethod of choice proved to be the reaction ofdipeptide-derived imidazolide with the potassium saltof malonic half esters in the presence of MgCl2.
    Type of Medium: Electronic Resource
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  • 4
  • 5
    ISSN: 1432-1890
    Keywords: Keywords Arbuscular-mycorrhiza ; Infectivity ; Fungal isolate ; Salt stress ; Symbiotic efficiency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The purpose of this study was to compare the effect of salinity on the symbiotic efficiencies and mycelial infectivity of two arbuscular mycorrhizal fungi (AMF), one isolated from saline soils (Glomus sp.) and the other (Glomus deserticola) from nonsaline soils (belonging to the Estación Experimental del Zaidín collection). Lettuce plants inoculated with either of these two fungi or maintained as uninoculated controls were grown in soil with three salt concentrations (0.25, 0.50 or 0.75 g NaCl kg–1 dry soil). Both AMF protected host plants against salinity. However, when the results of shoot dry weight and nutrient contents were expressed relative to the total length of mycorrhiza formed, it was found that both AMF differed in their symbiotic efficiencies. These differences were more evident at the two highest salt levels. Glomus sp.-colonized plants grew less and accumulated less N and P, whereas they formed a higher amount of mycorrhiza. The mechanism by which Glomus sp. protected plants from the detrimental effects of salt was based on the stimulation of root development, while the effects of G. deserticola were based on improved plant nutrition. The increase in salinity of soil decreased the hyphal growth and/or viability of Glomus sp. to a higher extent than those of G. deserticola since the mycelial network generated by G. deserticola was more infective than that of Glomus sp.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Journal of cryptology 13 (2000), S. 397-416 
    ISSN: 1432-1378
    Keywords: Key words. Undeniable signatures, RSA, Zero-knowledge.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Notes: Abstract. We present the first undeniable signatures scheme based on RSA. Since their introduction in 1989 a significant amount of work has been devoted to the investigation of undeniable signatures. So far, this work has been based on discrete log systems. In contrast, our scheme uses regular RSA signatures to generate undeniable signatures. In this new setting, both the signature and verification exponents of RSA are kept secret by the signer, while the public key consists of a composite modulus and a sample RSA signature on a single public message. Our scheme possesses several attractive properties. First, provable security, as forging the undeniable signatures is as hard as forging regular RSA signatures. Second, both the confirmation and denial protocols are zero-knowledge. In addition, these protocols are efficient (particularly, the confirmation protocol involves only two rounds of communication and a small number of exponentiations). Furthermore, the RSA-based structure of our scheme provides with simple and elegant solutions to add several of the more advanced properties of undeniable signatures found in the literature, including convertibility of the undeniable signatures (into publicly verifiable ones), the possibility to delegate the ability to confirm and deny signatures to a third party without giving up the power to sign, and the existence of distributed (threshold) versions of the signing and confirmation operations. Due to the above properties and the fact that our undeniable nsignatures are identical in form to standard RSA signatures, the scheme we present becomes a very attractive candidate for practical implementations.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: The obligate intracellular protozoan parasite Toxoplasma gondii depends on the purine-salvage pathway for its purine supply. Unlike its mammalian hosts, T. gondii salvages purine precursors predominantly via adenosine kinase, the enzyme that phosphorylates adenosine to adenosine monophosphate (AMP). The cDNA encoding T. gondii adenosine kinase was subcloned and expressed in Escherichia coli. The recombinant protein was active in an in vitro enzyme assay over a broad pH range. It required a divalent cation for activity. The enzyme was inactivated by the addition of 1 µM mercuric chloride. The inactivation could be reversed by a reducing agent. The active recombinant protein was crystallized using sodium sulfate as precipitant at pH 8.0. The crystals diffract to 1.8 Å and belong to the monoclinic space group P21, with unit-cell parameters a = 47.5, b = 68.9, c = 57.0 Å, β = 100.3°. The calculated Vm based on one molecule per asymmetric unit is 2.38 Å3 Da−1.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 56 (2000), S. 937-944 
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: Rab proteins are small Ras-like GTPases which play important roles in regulating intracellular vesicle trafficking. The nucleotide-binding domain of Rab6 from the malaria parasite Plasmodium falciparum was crystallized with GDP bound to the active site. The MAD phasing technique was used to determine the crystal structure to 2.3 Å resolution. Comparisons of the structure of GDP-bound PfRab6 with the recently determined structures of Rab3A in complex with either a GTP analog or with GTP and Rabphillin present structural evidence supporting the traditional model for the molecular GTP/GDP switch in Rab proteins. PfRab6 residues homologous to those distinguishing human Rab6 isoforms, which differ in binding to Rabkinesin-6 in human cells, are located next to the recognized complementarity-determining region (CDR) and constitute a conceptual broadening of that domain. Despite significant observable differences in Golgi ultrastructure, the Rab6 core structure and switch mechanism appear highly conserved when compared with murine Rab3a structures. A significant difference between the PfRab6 and higher eukaryotic Rabs may be the lack of CDR features that allow binding interactions with Rabkinesin-type effectors.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: The three-dimensional structure of the ternary complex consisting of human α-thrombin, hirugen and the active-site inhibitor RWJ-51438 has been determined at 1.7 Å resolution. The crystals of the complex belong to the orthorhombic space group P21212, with unit-cell parameters a = 62.98, b = 117.52, c = 47.99 Å. The refined R and Rfree values are 0.196 and 0.232, respectively. The ketone carbonyl group of the inhibitor is covalently linked to the hydroxyl O atom of Ser195, forming a tetrahedral intermediate hemiketal structure; the benzothiazole ring N atom of RWJ-51438 forms a hydrogen bond with His57. Surprisingly, the carboxylate substituent on the benzothiazole group forms salt bridges with Lys60F NZ and the NZ of the symmetry-related residues Lys236 and Lys240, which introduces steric effects that perturb the 60A–60I insertion loop, especially at residues Trp60D and Phe60H.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    [s.l.] : Macmillian Magazines Ltd.
    Nature 407 (2000), S. 355-358 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] When a quantum-mechanical system undergoes an adiabatic cyclic evolution, it acquires a geometrical phase factor in addition to the dynamical one; this effect has been demonstrated in a variety of microscopic systems. Advances in nanotechnology should enable the laws of quantum dynamics to ...
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