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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 154 (1997), S. 0 
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Termitomyces clypeatus secreted a 24-kDa xylanase constitutively in xylan medium, but required a gluconeogenic amino acid or Krebs cycle acid for the secretion of a 56-kDa amyloglucosidase in dextrin medium. Aspartate, glutamate, succinate and fumarate all increased secretion of amyloglucosidase from 50% to 〉90% and enzyme production by 10-fold with little effect on xylanase production. Glutamate or succinate stimulated in vitro release of intracellular amyloglucosidase from washed mycelia in the presence of cycloheximide. Amyloglucosidase accumulated in the absence of glutamate was a high-molecular-mass protein that did not migrate in PAGE. Cellular regulation by the fungus of the secretion of amyloglucosidase is indicated.
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  • 2
    ISSN: 1432-0967
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Abstract Pelitic rocks were thermally metamorphosed at the contact of the Chimakurthy mafic-ultramafic igneous complex, Eastern Ghats Belt, India. The rocks show progressive change in mineralogy from biotite-sillimanite-quartz-garnet-K-feldspar (association I, 150 m from the intrusive contact) to garnet-spinel-cordierite-K-feldspar-sillimanite (association II, 20–30 m from the intrusive contact) to cordierite-K-feldspar-(cordierite-orthopyroxene-K-feldspar symplectite after osumilite)-spinel-FeTiAl oxides with/without garnet (associations III and IV, 5 m from the intrusive contact), and finally to spinel-orthopyroxene-cordierite-K-feldspar (association V, xenoliths). Oxide mineral clots in associations III and IV resemble emery-type rocks. Initial mineral reactions involved biotite-dehydration melting with partial segregation of the melt. Down-temperature mineral reactions were largely diffusion controlled and preservation of symplectitic and coronitic textures in microdomains is common. Interpretation of reaction textures in relevant petrogenetic grids for the sytems KFMASH and FMAS and combined with geothermobarometry suggest that the pelitic rocks were thermally metamorphosed at c. 6 kbar pressure along a heating-cooling trajectory within the temperature interval between c. 750 °C and c. 1000 °C.
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  • 3
  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 168 (1997), S. 215-224 
    ISSN: 1432-072X
    Keywords: Key words Candida utilis ; Nitrite reductase ; Electron ; donors ; Cysteine modification ; Transcriptional control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The nitrate assimilation pathway in Candida utilis, as in other assimilatory organisms, is mediated by two enzymes: nitrate reductase and nitrite reductase. Purified nitrite reductase has been shown to be a heterodimer consisting of 58- and 66-kDa subunits. In the present study, nitrite reductase was found to be capable of utilising both NADH and NADPH as electron donors. FAD, which is an essential coenzyme, stabilised the enzyme during the purification process. The enzyme was modified by cysteine modifiers, and the inactivation could be reversed by thiol reagents. One cysteine was demonstrated to be essential for the enzymatic activity. In vitro, the enzyme was inactivated by ammonium salts, the end product of the pathway, proving that the enzyme is assimilatory in function. In vivo, the enzyme was induced by nitrate and repressed by ammonium ions. During induction and repression, the levels of nitrite reductase mRNA, protein, and enzyme activity were modulated together, which indicated that the primary level of regulation of this enzyme was at the transcriptional level. When the enzyme was incubated with ammonium salts in vitro or when the enzyme was assayed in cells grown with the same salts as the source of nitrogen, the residual enzymatic activities were similar. Thus, a study of the in vitro inactivation can give a clue to understanding the mechanism of in vivo regulation of nitrite reductase in Candida utilis.
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  • 5
    Electronic Resource
    Electronic Resource
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 106 (1997), S. 9703-9707 
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: Portions of the [CH2NO2] potential energy surface related to the OH+HNCO reaction were calculated by means of ab initio molecular orbital theory at the QCISD(T)/6-311++G(d,p) level based on UMP2/6-31G(d,p) optimized geometries. Of all possible three channels considered, the hydrogen abstraction turns out to be the dominant reaction channel. The addition to C atom requires activation energy slightly larger than that of the abstraction but smaller than that of the N addition, in contrast to the H+HNCO reaction. The structural and energetic parameters for the channels thus characterized were further utilized for the calculation of rate constants in the framework of a quantum statistical theory (QRRK). The contributions of the individual reaction channel towards the total rate constant have been examined. Although the OH+HNCO→NH2+CO2 reaction is more exothermic than the hydrogen abstraction OH+HNCO→H2+NCO, it is confirmed that rate constant for CO2 loss is much lower than that of H2O-elimination. The standard heat of formation of the adduct HNC(OH)O is estimated to be ΔHf298=−41.1±3 kcal/mol. © 1997 American Institute of Physics.
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  • 6
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 81 (1997), S. 7396-7408 
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Based on previously developed melt processing methods, Y2BaCuO5 (211) precipitates with various size were obtained in YBa2Cu3Ox (123). In particular, a wide distribution of 211 size from 100 Å to 10 μm has been found to exist in the matrix of 123, and strongly effected the critical current density, Jc. An enhancement of Jc was always found to be associated with the presence of extremely fine 211 precipitates. The flux pinning by these precipitates can be understood by considering the interaction of flux lines with cavities. It was found that although the flux lines are strongly pinned by cavities, the effectiveness decreases with the increasing number of flux lines trapped by them. © 1997 American Institute of Physics.
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Geophysical prospecting 45 (1997), S. 0 
    ISSN: 1365-2478
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences , Physics
    Notes: A new spectral factorization method is presented for the estimation of a causal as well as a causally invertible ARMA operator from the correlation sequence of seismic traces. The method has been implemented for multichannel deconvolution of seismic traces with the aim of exploiting the trace-to-trace correlation that exists within seismograms. A layered earth model with a small reflectivity sequence has been considered, and the seismic traces have been considered as the output of a linear system driven by white noise reflection coefficient sequences.  The present method is the concatenation of three algorithms, namely Kung's method for state variable (F,G,H) realization using a singular value decomposition (SVD) algorithm, Faurre's technique for computation of the strong spectral factor and Leverrier's algorithm for ARMA representation of the spectral factor. The inverted ARMA operator is used as a recursive filter for deconvolution of seismic traces. In the example shown, two traces with a covariance sequence of 160 ms length have been considered for multichannel deconvolution of stacked seismic traces. The results presented, when compared with those obtained from a conventional deconvolution algorithm, have shown encouraging prospects.
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  • 8
    ISSN: 1525-1314
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences
    Notes: A suite of garnet-wollastonite-scapolite-bearing calcsilicate granulites from the Eastern Ghats has been investigated to document the controls of mineral reactions during the metamorphic evolution of the deep continental crust. The rocks studied show heterogeneity in modal mineralogy and phase compositions in millimetre-sized domains. Textural relations, and the compositional plots of the phases, established that the clinopyroxene exerts a strong influence on the formation and composition of garnet in the complex natural system. P-T estimates using the vapour-independent equilibria involving garnet define a near isobaric cooling path from c. 850C at c. 5.5–5.2 kbar. The deduced trajectory tallies well with the terminal segment of the overall retrograde P-T path construed from the associated rocks using well-calibrated thermobarometers. The ubiquitous occurrence of wollastonite and scapolite in the main calcsilicate body suggests low aCO2 during peak metamorphic condition. Fluid compositions constrained from mineral-fluid equilibria of the garnet-bearing assemblages show domainal variations as a function of the compositions of the solid phases, e.g. garnet and clinopyroxene. A quantitative log/CO2-log/O2 diagram has been constructed to depict the stability of the different calcsilicate assemblages as functions of the compositions and the behaviour of these fugitive species. The results of the mineral-fluid equilibria and the quantitative fluid/rock ratio calculations, in conjunction with the topological constraints, imply vapour-deficient meta-morphism in the rocks studied. It is argued that fO2 during peak metamorphism was monitored by the ambient fO2. Subsequently, during retrogression, different domains evolved independently, whereas the fluid composition was controlled by the mineral-fluid equilibria.
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  • 9
    Electronic Resource
    Electronic Resource
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 71 (1997), S. 78-80 
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: In this letter, we describe the characteristics of molecular beam epitaxy GaAs/AlGaAs quantum-well infrared photodetectors (QWIP's) grown on a GaAs substrate, and on a GaAs-on-Si substrate produced by metalorganic chemical-vapor deposition. Important issues for QWIP applications such as dark current, spectral response, and absolute responsivity are studied. We find that compared to a similar detector structure grown on a GaAs substrate, the detector grown on a GaAs-on-Si substrate exhibits similar dark current and absolute responsivity while displaying a small blueshift in the spectral response. © 1997 American Institute of Physics.
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  • 10
    Electronic Resource
    Electronic Resource
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 70 (1997), S. 3573-3575 
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: The effect of laser annealing on important detector characteristics such as dark current, spectral response, and absolute responsivity is investigated for bound-to-continuum GaAs/AlGaAs quantum-well infrared photodetectors (QWIPs) operating in the 8–12 μm wavelength regime. A set of experiments was conducted on QWIPs fabricated from both as-grown and laser-annealed multiple-quantum-well structures. Compared to the as-grown structure, the peak spectral response of the laser-annealed structure was shifted to longer wavelengths, though absolute responsivity was decreased by about a factor of two. In addition, over a wide range of bias levels, the laser-annealed QWIPs exhibited a slightly lower dark current compared to the as-grown QWIPs. Thus, the postgrowth control of GaAs/AlGaAs quantum-well composition profiles by laser annealing offers unique opportunities to fine tune various aspects of a QWIP's response. © 1997 American Institute of Physics.
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