ISSN:
1432-0983
Keywords:
Saccharomyces cerevisiae
;
Isocitrate lyase
;
Gene regulation
;
Ethanol induction
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The ICL1 gene encoding the isocitrate lyase from Saccharomyces cerevisiae was cloned and sequenced. A reading frame of 557 amino acids showing significant similarity to isocitrate lyases from seven other species could be identified. Construction of icl1 null mutants led to growth defects on C2 carbon sources while utilization of sugars or C3 substrates remained unaffected. Using an ICL1-lacZ fusion integrated at the ICL1 locus, a more than 200-fold induction of β-galactosidase activity was observed after growth on ethanol when compared with glucose-repressed conditions. A preliminary analysis of the ICL1 upstream region identified a 364-bp fragment necessary and sufficient for this regulatory phenotype. Sequence motifs also present in the upstream regions of co-regulated genes were found within this region.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00312621
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