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  • 1990-1994  (2)
  • 1993  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Production of asymmetric hybrids between Solanum tuberosum and irradiated S. brevidens (1993)
    Springer
    Theoretical and applied genetics 85 (1993), S. 729-734 
    Details
    ISSN: 1432-2242
    Keywords: Protoplast fusion ; Gamma irradiation ; Partial genome transfer ; Solanum tuberosum ; Solanum brevidens
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Asymmetric somatic hybrids were obtained by fusion of Solanum tuberosum (PDH40) protoplasts with 300- or 500-Gy irradiated protoplasts of S. brevidens. These radiation doses were sufficient to prevent the growth of the S. brevidens protoplasts. Putative hybrids were selected on the basis of phenotype from regenerated shoots and identified with a S. brevidens-specific probe. From these, 31 asymmetric hybrids were confirmed by morphological characteristics, isoenzyme patterns and RFLP analysis. The morphology of the asymmetric hybrids was intermediate between that of S. tuberosum and symmetric hybrids of both species (obtained without irradiation treatment). Chromosome counts from 17 asymmetric hybrids showed that the chromosome number of the hybrids ranged from 31 to 64. The asymmetric hybrids probably had one or two genome complements (i.e. either 24 or 48 chromosomes) from S. tuberosum and 7–22 chromosomes from S. brevidens. There was no clear correlation between the radiation dose and the degree of elimination of the S. brevidens genome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225012
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  • 2
    Electronic Resource
    Electronic Resource
    Analysis of the mitochondrial DNA of the somatic hybrids of Solanum brevidens and S. tuberosum using non-radioactive digoxigenin-labelled DNA probes (1993)
    Springer
    Theoretical and applied genetics 85 (1993), S. 1017-1022 
    Details
    ISSN: 1432-2242
    Keywords: Potato ; S. brevidens ; Mitchondrial DNA ; Somatic hybrids ; Non-radioactive labelling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mitochondrial (mt) DNAs of somatic hybrids obtained by electrical and chemical fusion of mesophyll protoplasts of S. brevidens and a dihaploid line of S. tuberosum PDH 40 were analysed by Southern hybridization using the digoxigenin-labelled mtDNA sequences nad5 or orf25. In the Southern analysis of the hybrid mtDNA probed with nad5, most of the 19 hybrids analyzed had an RFLP pattern similar, but not identical, to one of the parents, S. tuberosum, PDH40. Nineteen percent of the hybrids had most of the S. brevidens fragments. Five of the hybrids had an identical RFLP pattern to either one of the parents while another two hybrids had novel RFLP patterns. Similar results were obtained by Southern analysis with orf25. These results clearly show that mtDNA rearrangements had occurred at a high frequency in the somatic hybrids. There were no differences in the frequencies of rearrangements observed between the hybrids regenerated from chemical and electrical fusions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00215042
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