ISSN:
1750-3841
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
A method for the extraction and purification of saffron secondary metabolites was developed. Among the solvents checked water was the most convenient for picrocrocin, 2,6,6-trimethyl-4-hydroxy-1-car-boxaldehyde-1-cyclohexene (HTCC) and crocin extraction from saffron stigmas. The compounds were separated by analytical and preparative thin layer chromatography on aluminum oxide, and identified by spectroscopic techniques. Quantitative determination was performed by high performance liquid chromatography methods with reverse phase C-18. Picrocrocin, HTCC and crocin isolated by preparative TLC showed a chromatographic purity of 98%, 96% and.70%, isolation yields being 88, 98 and 70%, respectively. The isolated compounds may be useful as food colorants.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2621.1992.tb08079.x
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