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  • DNA replication  (1)
  • 1990-1994  (1)
  • 1990  (1)
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  • 1990-1994  (1)
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  • 1
    Electronic Resource
    Electronic Resource
    Partial purification and characterization of a DNA helicase from chloroplasts of Glycine max (1990)
    Springer
    Plant molecular biology 15 (1990), S. 457-464 
    Details
    ISSN: 1573-5028
    Keywords: chloroplasts ; DNA helicase ; DNA replication
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A DNA helicase activity was detected in extracts of purified chloroplasts from the SB-1 cell line of Glycine max and partially purified by column chromatography on DEAE cellulose, phosphocellulose, and single-stranded DNA cellulose. The chloroplast helicase has a DNA-dependent ATPase activity, and its strand displacement activity is strictly dependent upon the presence of a nucleoside triphosphate and Mg2+ or Mn2+. Strand displacement activity does not require a free unannealed single-strand or replication fork-like structure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00019162
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