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  • 1
    Electronic Resource
    Electronic Resource
    Chronic exposure of goldfish-derived cell cultures to toxaphene alters the replication of Goldfish Virus-2 (1982)
    Springer
    Bulletin of environmental contamination and toxicology 29 (1982), S. 731-733 
    Details
    ISSN: 1432-0800
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01606114
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  • 2
    Electronic Resource
    Electronic Resource
    Uptake and toxicity of toxaphene to cell cultures derived from goldfish (Carassius auratus) (1982)
    Springer
    Bulletin of environmental contamination and toxicology 29 (1982), S. 68-75 
    Details
    ISSN: 1432-0800
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01606091
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  • 3
    Electronic Resource
    Electronic Resource
    Toxicity of certain insecticides to protozoa (1982)
    Springer
    Bulletin of environmental contamination and toxicology 28 (1982), S. 628-631 
    Details
    ISSN: 1432-0800
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01605596
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  • 4
    Electronic Resource
    Electronic Resource
    Cytogerontology since 1881: A reappraisal of August Weismann and a review of modern progress (1982)
    Springer
    Human genetics 〈Berlin〉 60 (1982), S. 101-121 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Cytogerontology, the science of cellular ageing, originated in 1881 with the prediction by August Weismann that the somatic cells of higher animals have limited division potential. Weismann's prediction was derived by considering the role of natural selection in regulating the duration of an organism's life. For various reasons, Weismann's ideas on ageing fell into neglect following his death in 1914, and cytogerontology has only reappeared as a major research area following the demonstration by Hayflick and Moorhead in the early 1960s that diploid human fibroblasts are restricted to a finite number of divisions in vitro. In this review we give a detailed account of Weismann's theory, and we reveal that his ideas were both more extensive in their scope and more pertinent to current research than is generally recognised. We also appraise the progress which has been made over the past hundred years in investigating the causes of ageing, with particular emphasis being given to (i) the evolution of ageing, and (ii) ageing at the cellular level. We critically assess the current state of knowledge in these areas and recommend a series of points as primary targets for future research.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00569695
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  • 5
    Electronic Resource
    Electronic Resource
    Isolation of paraquat-tolerant mutants from tomato cell cultures (1982)
    Springer
    Theoretical and applied genetics 63 (1982), S. 169-176 
    Details
    ISSN: 1432-2242
    Keywords: Tomato ; Cell cultures ; Herbicidetolerant mutant ; Methyl viologen ; Paraquat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Tomato callus clones selected for the ability to grow at paraquat concentrations lethal to wild-type cells were found at an approximate frequency of 5×10−8 per viable cell. Diploid plants were regenerated from nine of the nineteen paraquat-tolerant callus clones isolated. Although some of these plants appeared normal, others had altered morphology and reduced vigor and fertility. New callus cultures initiated from these regenerated plants typically had at least a 30-fold increase over the wild type in tolerance to paraquat. Tests on callus from sexual progeny showed that the paraquat-tolerant phenotypes of clones PQT4, PQT6, and probably also PQT13 resulted from dominant nuclear mutations, but the number of loci involved is not yet known. Paraquat spray experiments indicated that slight paraquat-tolerance was expressed at the plant level in PQT13, but not in any of the other clones tested.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00303704
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  • 6
    Electronic Resource
    Electronic Resource
    Human β-galactosidase and α-neuraminidase deficient mucolipidosis: Genetic complementation analysis of the neuraminidase deficiency (1982)
    Springer
    Human genetics 〈Berlin〉 60 (1982), S. 158-162 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Human β-galactosidase and α-neuraminidase deficient mucolipidosis [ML(gal-neur-)] is an inherited lysosomal enzymopathy which recently was designated as a sialidosis. We analyzed the neuraminidase deficiency of this disorder with genetic complementation analyses using a heterokaryon enrichment procedure. The genetic defects of two apparent variants of this disorder complemented the defects of the neuraminidase deficiency diseases, sialidosis I and mucolipidosis I, resulting in the restoration of neuraminidase activity in heterokaryons. The neuraminidase deficiency, therefore, may not be the primary defect in ML(gal-neur-) and is not an appropriate test for determining carrier status. The clinical and biochemical characteristics of this disorder suggest that a post-translational or processing event for these enzymes may be defective. The defect, however, is different from I-cell disease and pseudo-Hurler polydystrophy, two disorders of post-translational lysosomal enzyme biosynthesis, since complementation studies demonstrated recovery of intracellular β-galactosidase and α-neuraminidase levels in heterokaryons. The lack of human β-galactosidase expression in man-mouse somatic cell hybrids formed from fibroblasts of the infantile onset type disorder suggests that the defect is not corrected by the mouse genome. The ML(gal-neur-) disorder therefore appears to be a distinct subtype of the inherited neuraminidase deficiencies in which the defect may occur in a post-translational or regulatory step which coordinately affects the expression of lysosomal β-galactosidase and α-neuraminidase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00569704
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  • 7
    Electronic Resource
    Electronic Resource
    Coronavirus 229E susceptibility in man-mouse hybrids is located on human chromosome 15 (1982)
    Springer
    Somatic cell and molecular genetics 8 (1982), S. 83-94 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Human coronavirus 229E, an enveloped, RNA-containing virus, causes respiratory illness in man and is serologically related to murine coronavirus JHM, which causes acute and chronic demyelination in rodents. 229E displays a species-specific host range restriction whose genetic basis was studied in human-mouse hybrids. 229E replicated in human WI-38 cells but not in three mouse cell lines tested (RAG, LM/TK−, and A9). Human coronavirus sensitivity (HCVS) was expressed as a dominant phenotype in hybrids, indicating that mouse cells do not actively suppress 229E replication. HCVS segregated concordantly with the human chromosome 15 enzyme markers mannose phosphate isomerase (MPI) and the muscle form of pyruvate kinase (PKM2), and analysis of hybrids containing an X/15 translocation [t(X;15)(p11;q11)] localized HCVS to the q11 → qter region of chromosome 15. HCVS might code for a specific surface receptor, allowing 229E to be absorbed to and received within the host cell.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01538652
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  • 8
    Electronic Resource
    Electronic Resource
    A simple method for obtaining cultures enriched for Type II pneumonocytes from fetal rabbit (1982)
    Springer
    Methods in cell science 7 (1982), S. 63-68 
    Details
    ISSN: 1573-0603
    Keywords: fetal lung ; Type II cells ; culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A differential plating method permitted preparation of cultures significantly enriched for Type II pneumocytes. These cells were maintained in a differentiated state for at least 12 d, identifiable morphologically (by presence of osmiophilic lamellar inclusion bodies) and bio-chemically (by demonstration of synthesis of phosphatidyl choline and production of disaturated lecithin).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01665911
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  • 9
    Electronic Resource
    Electronic Resource
    Establishment of epithelial cell lines from individual rabbit lenses (1980)
    Springer
    Methods in cell science 6 (1980), S. 57-60 
    Details
    ISSN: 1573-0603
    Keywords: rabbit ; lens epithelium ; primary culture ; cell line ; lens crystallins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We describe a method of initiating primary cell cultures from lenses of rabbits by an explant-outgrowth technique. These primary cultures routinely yield epithelial cell lines when subcultured with collagenase-trypsin chicken serum. Most lines examined retain the diploid chromosome number through at least 30 passages.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01666017
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  • 10
    Electronic Resource
    Electronic Resource
    Expression of H1 histone genes in mouse-human somatic cell hybrids (1980)
    Springer
    Somatic cell and molecular genetics 6 (1980), S. 653-665 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The synthesis of H1 histones was studied in nine mouse-human somatic cell hybrid clones containing reduced numbers of human chromosomes. The entire human genome could be accounted for karyologically and by the use of functional assays for specific enzyme markers encoded by human chromosomes. Chromatographic resolution and peptide mapping of species-specific H1 histones failed to reveal human H1 histones to a level of about 1% of total in the nine clones. In addition to the species-specific extinction of human H1 histones, effects were seen on the quantity of mouse H1 histone subtypes produced in four of the nine clones. The remaining five clones produced H1 histones qualitatively and quantitatively identical with those of the mouse parent, which was common to all nine clones. The results suggest at least two levels of control for H1 histone gene expression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01538644
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