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  • 1
    ISSN: 1432-072X
    Keywords: Phototrophic bacteria ; Ectothiorhodospira vacuolata ; Gas vacuoles ; Alkaliphilic bacteria ; Halophilic bacteria ; Anaerobic sulfide oxidation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A new phototrophic bacterium was isolated from Jordanian and Kenyan alkaline salt lakes. Cells are rod shaped, 1.5 μm wide and 2–4 μm long, and motile by polar flagella. They divide by binary fission, and possess photosynthetic membranes as lamellar stacks similar to those in the other species of the genus Ectothiorhodospira and the brown colored Rhodospirillum species. The presence of bacteriochlorophyll a and carotenoids of the normal spirilloxanthin series is indicated by the absorption spectra of living cells. Under certain growth conditions the cells form gas vacuoles, may become immotile and float to the top of the culture medium. Sulfide and thiosulfate are used as photosynthetic electron donors. During the oxidation of sulfide to sulfate, elemental sulfur is formed, which is accumulated outside the cells. The organisms are strictly anaerobic, do not require vitamins, are moderately halophilic and need alkaline pH-values for growth. The new species Ectothiorhodospira vacuolata is proposed.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 130 (1981), S. 234-237 
    ISSN: 1432-072X
    Keywords: Rhodospirillaceae ; Rhodopseudomonas globiformis ; Sulfate assimilation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Rhodopseudomonas globiformis is able to grow on sulfate as sole source of sulfur, but only at concentrations below 1 mM. Good growth was observed with thiosulfate, cysteine or methionine as sulfur sources. Tetrathionate supported slow growth. Sulfide and sulfite were growth inhibitory. Growth inhibition by higher sulfate concentrations was overcome by the addition of O-acetylserine, which is known as derepressor of sulfate-assimilating enzymes, and by reduced glutathione. All enzymes of the sulfate assimilation pathway. ATP-sulfurylase, adenylylphosphate-sulfotransferase, thiosulfonate reductase and O-acetylserine sulfhydrylase are present in R. globiformis. Sulfate was taken up by the cells and the sulfur incorporated into the amino acids cysteine, methionine and homocysteine. It is concluded, that the failure of R. globiformis to grow on higher concentrations of sulfate is caused by disregulation of the sulfate assimilation pathway. Some preliminary evidence for this view is given in comparing the activities of some of the involved enzymes after growth on different sulfur sources and by examining the effect of O-acetylserine on these activities.
    Type of Medium: Electronic Resource
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  • 3
    Publication Date: 1981-11-01
    Print ISSN: 0302-8933
    Electronic ISSN: 1432-072X
    Topics: Biology
    Published by Springer
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  • 4
    Publication Date: 1981-11-01
    Print ISSN: 0302-8933
    Electronic ISSN: 1432-072X
    Topics: Biology
    Published by Springer
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  • 5
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    Springer
    In:  Archives of Microbiology, 130 (3). pp. 234-237.
    Publication Date: 2016-01-21
    Description: Rhodopseudomonas globiformis is able to grow on sulfate as sole source of sulfur, but only at concentrations below 1 mM. Good growth was observed with thiosulfate, cysteine or methionine as sulfur sources. Tetrathionate supported slow growth. Sulfide and sulfite were growth inhibitory. Growth inhibition by higher sulfate concentrations was overcome by the addition of O-acetylserine, which is known as derepressor of sulfate-assimilating enzymes, and by reduced glutathione. All enzymes of the sulfate assimilation pathway. ATP-sulfurylase, adenylylphosphate-sulfotransferase, thiosulfonate reductase and O-acetylserine sulfhydrylase are present in R. globiformis. Sulfate was taken up by the cells and the sulfur incorporated into the amino acids cysteine, methionine and homocysteine. It is concluded, that the failure of R. globiformis to grow on higher concentrations of sulfate is caused by disregulation of the sulfate assimilation pathway. Some preliminary evidence for this view is given in comparing the activities of some of the involved enzymes after growth on different sulfur sources and by examining the effect of O-acetylserine on these activities.
    Type: Article , PeerReviewed
    Format: text
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  • 6
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    Urban & Fischer
    In:  Zentralblatt für Bakteriologie: I. Abt. Originale C: Allgemeine, angewandte und ökologische Mikrobiologie, 2 (3). pp. 228-234.
    Publication Date: 2015-03-24
    Description: A new species of the genus Ectothiorhodospira is described isolated from alkaline soda lakes of the Wadi Natrun in Egypt. In most of its properties it resembles the recently described Ectothiorhodospira halochloris, which had been isolated from similar lakes of the same area. The new species, Ectothiorhodospira abdelmalekii, has spiral-shaped cells with 0.9-1.2 μm diameter, it is polarly flagellated with sheated flagella, has lamellar photosynthetic membrane stacks, possesses bacteriochlorophyll b as the main photosynthetic pigment, and has a DNA base composition of 63.3–63.8 mol% G + C. Photolithotrophic growth with sulfide or elemental sulfur as electron donors is the predominant mode of life. During sulfide oxidation to sulfate, elemental sulfur is accumulated outside the cells. Acetate, pyruvate, fumarate and succinate are photoassimilated. The properties of E. halochloris and E. abdelmalekii are compared.
    Type: Article , PeerReviewed
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