ALBERT

Notes: Egg white was subjected to proteolysis by ficin, bromelin, papain, trypsin and protease; changes in functional properties were monitored. Many of the changes observed were due to the addition of enzyme rather than actual proteolysis (e.g., foam volume increase, foam stability decrease, and angel cake volume increase). The angel cake volume showed a further increase with proteolysis; however, off-flavors were also noted. In experiments with ficin and bromelin, proteolysis by both enzymes decreased the coagulum strength while only ficin decreased the amount of noncoagulable protein.

Notes: Abstract: A model that incorportaes performace data about data about several wastewater management systems is discussed. From these data the excepted behavior of an individual wastewater system or group of systems can be product of the performace probabilities of its individual components. The modeel can be used on a regional scale facilitating land use planning by allowing accurate estimates of performance for a prospective wastewater management system. At this scale it can allow the impact evaluation of new wastewater technology on land use in a region.

Notes: SYNOPSIS. Heme, intrinsically required by Trypanosomatidae, is unstable, especially in conventional alkaline (pH 7.2–8.0) media. Low solubility of heme in a pH 6.5 basal medium (developed to assay biopterin with Crithidia fasciculata) posed a problem: in media acidified during growth because of glycolysis, heme precipitated, perhaps contributed to acid-limited growth and interfered with densitometric estimation of growth. The remedy was to: replace glucose with less rapidly metabolized mannitol; distribute media in thin layers to promote oxidation of acetate, fumarate, and malate (presumably leaving an alkaline residue); and buffer heavily with histidine + Good zwitterionic buffers, and superimpcse physiological buffering by arginine + asparagine whose catabolism appeared to yield an excess of NH+4 over acid. Thereupon, Fe and Cu deficiencies sharply limited growth in the medium whose main chelators were: (a) 2,3–dihydroxybenzoic + 5-sulfosalicylic acids (which preferentially bind transitional elements at their higher valences; (b) malic and gluconic acids; and (c) histidine. With unconventionally heightened concentrations of Fe, Cu, and Mo (the latter serving as Cu buffer as well as nutrient per se), the hemin concentration could be lowered, widening the margin of safety for heme solubility. Growth then reached 1.4 × 108 cell/ml. This medium may serve to screen for ligands promoting uptake or release of Fe and Cu. The increased growth is a step towards improving the assay medium for biopterin and practical use of Crithidia to assay several B vitamins and essential amino acids for metazoa.

Notes: Light-trap catches of five tortricid species were recorded at orchard sites in Kent over an 11-year period. The dates on which moths of Hedya nubiferana (Haw.), Cydia pomonella (L.) and Archips podana (Scop.) were caught in light traps were strongly correlated with accumulated temperatures in day-degrees above a base of 8°C. The correlation was less good for catches of Pandemis heparana (Denis & Schiff.) and it was slightly better for those of Cydia pomonella above 10°C than above 8°C. The data were insufficient to calculate temperature sums for catches of Adoxophyes orana (Fisch. v. Rösl.). Moth catches of all five species showed differences between years in the timing of first and second generations. The dates of first catches are compared with dates estimated from the following temperature sums: for H. nubiferana 226 day-degrees above 8°C, for C. pomonella 268 day-degrees above 8°C and 149 day-degrees above 10°C, for Archips podana 326 day-degrees above 8°C and for P. heparana 386 day-degrees above 8°C. In the 11-year period, estimated dates of first catch were within ± 5 days of first actual catch of moths in light traps in nine years for C. pomonella, eight years for H. nubiferana and A. podana, but only five years for P. heparana.

Notes: Several degradation products of the iriazine herbicides atrazine [2-chloro-4-ethylamino-6-isapropylainino-1,3,5-triazine], cyprazine [2-chloro-4-cyclopropylamino-6-isopropylamino-1,3,5-triazine] and cyanazine [2-chloro-4-(l-cyanomethylethyl-amimo)-6-ethylamino-1,3,5-triazine] were monitored over a 3-year period in soil Trom fields under maiTC production. The soils were predominately loamy sands and sandy clay loams. HydroKy-triazines were determined semi-quanlilatively m the soil samples by gaschromalography, after methylation. Their levels ranged Trom 0.05 to 0.5 ppm. The hydroxy-triazines were the predominant triazine residues in the field during ihe spring and autumn, N-de-ethylated atrazine [2-chloro-4-amino-6-iso-propylamino-1,3,5-triazine] persisted at relatively high levels (0.015–0.02 ppm) 12 months after the application of atrazine. Greater proportions of N-de-ethylated atrazine and cyanazine II [2-chloro-4-(1-carbamoyl-1-methyleihylamino)-6-ethyl-amino-s-iriazine] than of atrazine or cyanazine were found to move through the soil profile into subsurface drainage water (1.2–1.6 m depth). Abbau und Transport von drei Triazin-Herbiziden und einiger ihrer Abbauproduk te im Boden unter Feldhedingungen Boden von Maisfeldern wurde über eine Periode von drei Jahren auf Abbauprodukte folgender Triazin-Herbizide unter-suchi: Atrazin [2-Chlor-4-äthylamino-6-isopropylatnino-1,3,5-triazin], Cyprazin [2-Chlor-4-cyclopropylamino-6-isopropyla-mino-1,3,5-(triazin) und Cyanazin [2-Chlor-4-(1-cyanoinethy-läthylamino)-6-äthylamino-1,3,5-triazin], Bei den Böden han-delie es sich hauptsächlich um lehmige Sande und sandig-tonige Lehme Hydroxy-Triazine wurden in den Bodenproben gas-chromatogruphish nach Methylierung. semi-quantitativ be-stimmt. Ihre Konzeniration im Boden betrug zwischen 0.05 und 0.5 ppm. Die Hydroxy-Triazine waren im Frähjahr und Herbst die vorherrschenden Rücksiände, N-desäthylieries Airazin [2-Chlor-4-amino-6-isopropyIamino-1,1,5-triazin] war zwölf Monate nach der Atrazin-Applikation mit relative hohen Kon-zenirationen(0.015–0.02 ppm) vorhanden. Von N-desäthylier-tem Airazin und Cyanazin II [2-Chlor-4-(1 carbamoyl-1–methy-läthylamino)-6-äthylamino-s-triazin] wurden grössere Anteile im Boden in das unterirdische Drainagewasser transportiert (1.2–1.6 m Tiefe) als es für Atrazin oder Cyanazin der Fs Swiir Disparition et migration dans le sol, au champ, de trois triazines herhicides et de plusieurs de leurs produits de dégradalion. Plusieurs produits de dégradation des triazines herbicides suivantes: atrazine (2-chloro-4-éthylamino-6-isopropylamino-1,3,5-triazine) cyprazine (2chloro-4-cyclopropylamino-6-isopropylamino-l,3,5-triazine) et cyanazine (2-chloro-4 (l-cyanométhyléthylamino)-6-éthylamino-l,3,5-triazine) ont été contrölés dans le sol. pendant une période de trois années, dans des champs de maïs en production, Les sols étaient pour la plupart sablo-limoneux et limono-argilo-sableux, Les hydroxy-triazines ont été déterminées semi-quantalivcmeni dans les échantillons de sols, par chroma tographie gazeuse aprés méthy lation, Leursconcen [rations s'étendaienlde0.05à 0.5ppm, Les hydroxy-triazines ont été les résidus les plus imporiants dans le champ au printemps et à I'aulomne, L'alrazine N-dé-éthylée [2-chloro-4-amino-6-isopropylamino-l.3.5-triazine] a persistéà des concentralions rclativemeni élevées (0.015 à 0.02 ppm) douzemois aprés l'application de l'atrazine, Il a été conslaté que des proportions d'atrazine N-dé-éthylée et de cyanazine II [2-ehloro-4(l-carbamoyl-1-méthyléthylamino)-6-éthylamino-1,3,5-triazine] plus importantes que celles de l'atrazine ou de la cyanazine, migraient à travers le profil du sol. dans la subsurface de drainage dc l'eau (1,2 à 1.6 m de profondeur).

Notes: Mechanically deboning several traditional and underutilized fish species increased the microbial count tenfold. The shelf life of the minced fish at 2° and 12°C was 5 and 3 days respectively with a corresponding total bacterial count of 1.0–5.0 × 108/g. The bacterial and Coliform counts during frozen storage (up to 3 mo, −25°C) showed little or no change. Frozen minced fish samples thawed from -25° to 2°C at thawing rates of 1.2, 1.6, 5.0, and 12.5°C/hr all had the same final bacterial count. Psychrotrophic bacteria in a beef-fish mixture stored at 2°C grew at the same rate as fish or beef alone. At 12°C the bacterial growth in the beef-fish mixture was slower than in beef or fish alone.