Publication Date:
2017-01-10
Description:
The 2-methylthio (ms 2 ) modification at A37 of tRNAs is critical for accurate decoding, and contributes to metabolic homeostasis in mammals. However, the regulatory mechanism of ms 2 modification remains largely unknown. Here, we report that cysteine hydropersulfide (CysSSH), a newly identified reactive sulfur species, is involved in ms 2 modification in cells. The suppression of intracellular CysSSH production rapidly reduced ms 2 modification, which was rescued by the application of an exogenous CysSSH donor. Using a unique and stable isotope-labeled CysSSH donor, we show that CysSSH was capable of specifically transferring its reactive sulfur atom to the cysteine residues of ms 2 -modifying enzymes as well as ms 2 modification. Furthermore, the suppression of CysSSH production impaired insulin secretion and caused glucose intolerance in both a pancreatic β-cell line and mouse model. These results demonstrate that intracellular CysSSH is a novel sulfur source for ms 2 modification, and that it contributes to insulin secretion.
Print ISSN:
0305-1048
Electronic ISSN:
1362-4962
Topics:
Biology
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