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  • 1
    Online Resource
    Online Resource
    A Practical Guide to Protein Engineering (2020)
    Cham :Springer International Publishing :
    Details
    Keywords: Proteins . ; Biotechnology. ; Biology Technique. ; Protein Biochemistry. ; Chemical Bioengineering. ; Biological Techniques.
    Description / Table of Contents: Literature Search -- Sequence Analysis -- Structural Analysis -- Protein Expression Hosts and Expression Plasmids -- Gene Cloning -- Protein Expression -- Assay -- Gene Mutagenesis -- High-Throughput Screening (HTS) -- Protein Variants Analysis and Characterization -- Continuing from Protein Variants -- Employability.
    Abstract: This textbook introduces readers in an accessible and engaging way to the nuts and bolts of protein expression and engineering. Various case studies illustrate each step from the early sequence searches in online databases over plasmid design and molecular cloning techniques to protein purification and characterization. Furthermore, readers are provided with practical tips to successfully pursue a career as a protein engineer. With protein engineering being a fundamental technique in almost all molecular biology labs, the book targets advanced undergraduates and graduate students working in molecular biology, biotechnology and related scientific fields.
    Type of Medium: Online Resource
    Pages: XV, 202 p. 91 illus., 68 illus. in color. , online resource.
    Edition: 1st ed. 2020.
    ISBN: 9783030568986
    Series Statement: Learning Materials in Biosciences,
    URL: https://doi.org/10.1007/978-3-030-56898-6
    DOI: 10.1007/978-3-030-56898-6
    DDC: 572.6
    Language: English
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  • 2
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    Comparing Methods and Defining Practical Requirements for Extracting Harmonic Tidal Components from Groundwater Level Measurements (2021)
    Springer Berlin Heidelberg
    Details
    Publication Date: 2024-03-06
    Description: The groundwater pressure response to the ubiquitous Earth and atmospheric tides provides a largely untapped opportunity to passively characterize and quantify subsurface hydro-geomechanical properties. However, this requires reliable extraction of closely spaced harmonic components with relatively subtle amplitudes but well-known tidal periods from noisy measurements. The minimum requirements for the suitability of existing groundwater records for analysis are unknown. This work systematically tests and compares the ability of two common signal processing methods, the discrete Fourier transform (DFT) and harmonic least squares (HALS), to extract harmonic component properties. First, realistic conditions are simulated by analyzing a large number of synthetic data sets with variable sampling frequencies, record durations, sensor resolutions, noise levels and data gaps. Second, a model of two real-world data sets with different characteristics is validated. The results reveal that HALS outperforms the DFT in all aspects, including the ability to handle data gaps. While there is a clear trade-off between sampling frequency and record duration, sampling rates should not be less than six samples per day and records should not be shorter than 20 days when simultaneously extracting tidal constituents. The accuracy of detection is degraded by increasing noise levels and decreasing sensor resolution. However, a resolution of the same magnitude as the expected component amplitude is sufficient in the absence of excessive noise. The results provide a practical framework to determine the suitability of existing groundwater level records and can optimize future groundwater monitoring strategies to improve passive characterization using tidal signatures.
    Description: H2020 Marie Sklodowska-Curie Actions http://dx.doi.org/10.13039/100010665
    Description: Swedish Research Council
    Keywords: ddc:551.49 ; Tidal subsurface analysis ; Tidal constituents ; Signal analysis ; Harmonic least squares ; Non-uniform sampling
    Language: English
    Type: doc-type:article
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    CITATION GEO-LEO
  • 3
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    Queer Spiritual Spaces (2022)
    Routledge
    Details
    Publication Date: 2023-07-19
    Description: Drawn from extensive, new and rich empirical research across the UK, Canada and USA, Queer Spiritual Spaces investigates the contemporary socio-cultural practices of belief, by those who have historically been, and continue to be, excluded or derided by mainstream religions and alternative spiritualities. As the first monograph to be directly informed by 'queer' subjectivities whilst dealing with divergent spiritualities on an international scale, this book explores the recently emerging innovative spaces and integrative practices of queer spiritualities. Its breadth of coverage and keen critical engagement mean it will serve as a theoretically fertile, comprehensive entry point for any scholar wishing to explore the queer spiritual spaces of the twenty-first century.
    Keywords: Social Science ; Anthropology ; bic Book Industry Communication::J Society & social sciences::JH Sociology & anthropology::JHM Anthropology
    Language: English
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