ALBERT

Notes: This study was conducted in order to develop a mathematical model for predicting lysine losses during processing of soy products. Model systems used in this study consisted of micro crystalline cellulose, glucose, sucrose, potato starch and soy protein. System composition, water activity, pH and time and temperature of heating were varied and available lysine was monitored by the fluoro-2,4-dinitro benzene method of Carpenter. In the first set of experiments, statistical analysis of the data obtained resulted in an equation which shows the effects of the variables used on available lysine retention: Fraction available lysine remaining = 0.58 + 0.047 pH - 0.093 glucose - 0.059 temperature —0.0068 time + 0.031 (water activity)2+ 0.025 aw sucrose + 0.033 sucrose starch. These data were collected at heating times well in excess of that required to destroy all reducing sugars present. Heating times were reduced in the second experiments such that kinetic data could be obtained. The average Ea was 28,500 calories/mole°K and the average reference reaction rate at 100°C (K100) was 0.036 mg lysine/min.

Notes: An improved analytical method for predicting the freezing time with one dimensional heat transfer for slabs was developed. Tylose- MH-1000 was used as a model test material. The new model is similar to Plank's equation, but has a more theoretical basis. Total enthalpy difference instead of latent heat and weighted average temperature difference instead of the temperature difference between initial freezing point and freezer temperature were used in the improved prediction method. Linear regression was used to estimate shape parameters. Four different foods were used to test the model. Predicted times for foods were within 6% of the measured times.

Notes: Several established methods of phytic acid determination in soybeans were evaluated. Iron analysis methods, which rely on a 4:6 molar ratio of Fe:P, were eliminated because this ratio was not dependable. Three assay methods relying on phosphorus analysis were then compared. The anion-exchange method was considered most accurate but not convenient for routine analysis. Analysis of the ferric phytate precipitate and a new method, analysis of the supernatant before and after ferric chloride precipitation, were judged against the anion-exchange method under different extraction conditions. Based upon good agreement with anion-exchange column results and acceptable reproducibility, the best methods were (1) precipitate analysis of phosphorus after extraction with 3% TCA + 10% sodium sulfate, or (2) the supernatant difference method after extraction with 1.2% HCl.

Notes: Methods were determined for the induction of diploid gynogenesis in the sole, Solea solea (L.), in order to investigate the sex-determining mechanism. The experiments utilized gametes obtained by dissection of fish caught at sea. Activation of eggs with UV-irradiated sole sperm was not feasible but development was initiated by exposure to halibut, Hippoglossus hippoglossus (L.), sperm. These embryos displayed the typical characteristics of haploids and few survived beyond hatching. High levels (〉 90%) of diploidy were induced in such eggs by subjecting them to a cold shock (2°C or 4°C) for 1-2 h about 10-15 min after activation at 12°C. The resultant embryos were indistinguishable from those developing from normally fertilized eggs and were considered to be diploid gynogenomes. Fifteen of these were reared to a length of 5-10 cm by which time gonad differentiation had been initiated. Both sexes were represented, indicating that sex in this species is not determined by a simple XX-XY system with female homogamy.

Notes: Lucerne (Medicago sativa L.) sown at 2·8-11·2 kg ha−1 in spring was thinned in the autumn by digging plants from alternate 15- or 30-cm lengths of row. Both thinning treatments decreased the subsequent year stand density by 34%. Yields the following year were reduced by 24 and 17% for the 15- and 30-cm treatments respectively, and the next year by 10% for both treatments. Thinning effects were similar at all sowing rates. During the year following thinning, plants did not grow larger in response to the thinning. Thus, development of compensatory growth by lucerne plants following sudden stand thinning is slow.

Notes: Six annual legumes were evaluated as components of cereal-grass-legume intercrops in two experiments at two sites differing in elevation by 789 m. Barley (Hordeum vulgare L.) and Westerwolds rye-grass (Lolium multiflorum Lam.) were seeded on all intercrop plots. Dry-matter (DM) yield, crude protein (CP) and organic matter digestibility (OMD) were measured. DM yield and N content were used to estimate legume N fixation. Experiment 1 was conducted at both sites. At the lower site, Persian clover (Trifolium resupinatum L.) and annual alfalfa (Medicago sativa L.) accounted for 70% of the DM yield in harvest 1 (July), increased CP and OMD, but did not affect intercrop yield. They increased harvest 2 (August/September) intercrop yield by 263% and CP concentration by 65 g kg−1 DM. They increased harvest 3 (October) yield by 275% and CP concentration by 78 g kg−1 DM. Inclusion of striate lespedeza (Lespedeza striata) did not affect intercrop yield or quality. Annual legumes failed to establish at the higher elevation site and therefore had no effect on DM yield or forage quality. In Experiment 2, in which the performance of Westerwolds ryegrass was also compared with that of Italian ryegrass, and conducted at the lower site only, Persian clover and berseem clover (T. alexandrinum L.) increased CP of all three of the year's harvests. These two species contributed 29% of the DM yield in the first harvest (July) but did not affect total intercrop yield. They increased harvest 2 (August) yield by 313%. Persian clover increased harvest 3 (October) yield by 318% and berseem clover increased harvest 3 yield by 405%. Barrel medic (Medicago truncatula) and snail medic (M. scutellata) contributed 29% of harvest 1 yield, and increased both DM yield and CP content. Medics did not regrow. Aubade Westerwolds ryegrass contributed a greater percentage of the DM yield than did Maris Ledger Italian ryegrass at harvests 1 and 2. Ryegrass type did not affect total DM yield but did affect forage quality; intercrops containing the Italian ryegrass had higher CP at harvest 2 and higher OMD at harvest 3 than those containing the Westerwolds ryegrass. Over both experiments, at the lower elevation site, stands with Persian clover, berseem clover or alfalfa produced 80% of the yield of barley-ryegrass receiving 250 kg N ha−1, and 165% of the yield of unfertilized barley-ryegrass. Berseem and Persian clover fixed about the same amount of N over the growing season; 188 kg N ha−1 in Experiment 1 and 134 kg N ha−1 in Experiment 2.