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  • 1
    Electronic Resource
    Electronic Resource
    ROOT-KNOT NEMATODE RESISTANCE GENES IN TOMATO AND THEIR POTENTIAL FOR FUTURE USE (1998)
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Phytopathology 36 (1998), S. 277-293 
    Details
    ISSN: 0066-4286
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Biology
    Notes: Abstract The gene Mi, which confers resistance to several species of root-knot nematode, is present in many modern tomato cultivars. Recent cloning of this gene revealed that it encodes a member of the plant resistance protein family characterized by the presence of a putative nucleotide binding site and a leucine-rich repeat. Analysis of transgenic plants revealed the unexpected result that Mi also confers resistance to potato aphids. Although highly effective in many conditions, Mi fails to confer resistance at high soil temperature, and Mi-virulent nematode isolates have been identified in many areas of the world. These findings have stimulated efforts to identify new sources of root-knot nematode resistance. Resistance genes that differ from Mi in properties and genetic position have been identified in Lycopersicon peruvianum. These genes, as well as the cloned Mi gene, provide a resource for broadening the base of root-knot nematode resistance in tomato and other crops.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1146/annurev.phyto.36.1.277
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    DNA sequences of two yeast promoter-up mutants (1983)
    [s.l.] : Nature Publishing Group
    Nature 304 (1983), S. 652-654 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The strategy used to clone the constitutively expressed ADR2 gene from the ADR3-4C and ADR3-5C mutants is outlined in Fig. 1. Yeast genomic DNA was isolated from the appropriate strain, digested with BamHI, and size-fractionated by agarose gel electrophoresis. Southern blot analysis of the digested ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/304652a0
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  • 3
    Electronic Resource
    Electronic Resource
    Sequence of a symbiont (2003)
    [s.l.] : Nature Publishing Group
    Nature biotechnology 21 (2003), S. 1294-1295 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] The bacterium P. luminescens is pathogenic to a wide range of insects. It is transmitted to the body cavity of an insect host by the nematode Heterorhabditis bacteriophora, an organism with which it has a complex symbiotic relationship. The ability of P. luminescens to switch from symbiont in the ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nbt1103-1294
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  • 4
    Electronic Resource
    Electronic Resource
    Isolation of the structural gene for alcohol dehydrogenase by genetic complementation in yeast (1980)
    [s.l.] : Nature Publishing Group
    Nature 283 (1980), S. 214-216 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The vector used for constructing the yeast DNA pool was the plasmid YRp7 (ref. 11). YRp7 is composed of a 1.4-kilobase EcoRl fragment containing the yeast TRP1 gene inserted into the EcoRl site of pBR322 (ref. 12). The fragment containing TRP1 confers on the hybrid plasmid the ability to replicate ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/283214a0
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  • 5
    Electronic Resource
    Electronic Resource
    Isolation ofCaenorhabditis elegans mutants lacking alcohol dehydrogenase activity (1991)
    Springer
    Biochemical genetics 29 (1991), S. 313-323 
    Details
    ISSN: 1573-4927
    Keywords: alcohol dehydrogenase ; allyl alcohol selection ; ADH mutants ; Caenorhabditis elegans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Alcohol dehydrogenase (ADH) and the genes encoding this enzyme have been studied intensively in a broad range of organisms. Little, however, has been reported on ADH in the free-living nematodeCaenorhabiditis elegans. Extracts of wild-typeC. elegans contain ADH activity and display a single band of activity on a native polyacrylamide gel. Reaction rate for alcohol oxidation is more rapid with higher molecular weight alcohols as substrate than with ethanol. Primary alcohols are preferred to secondary alcohols.C. elegans is sensitive to allyl alcohol, a compound that has been used to select for ADH-null mutants of several organisms. Allyl alcohol-resistant mutant strains were selected from ethylmethanesulfonate (EMS)-mutagenized nematode populations. ADH activity was measured in extracts from eight of these strains and was found to be low or nondetectable. These results form a basis for molecular and genetic characterization of ADH expression inC. elegans.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00554139
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  • 6
    Electronic Resource
    Electronic Resource
    Delta factor can displace sigma factor from Bacillus subtilis RNA polymerase holoenzyme and regulate its initiation activity (1978)
    Springer
    Molecular genetics and genomics 161 (1978), S. 135-141 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A protein with a molecular weight of 21,000 daltons is found associated with a fraction of Bacillus subtilis RNA polymerase core. This protein (δ) does not react with antibody made against sigma factor and has a peptide map which is significantly different from sigma factor. At ratios of 2:1 to 4:1 (δ:holoenzyme) the δ displaces sigma factor completely from the core and associates in a 1:1 ratio with core to form δ-core. Under the same incubation conditions sigma factor at a ratio of 10:1 (sigma factor: δ-core) does not displace δ from the δ-core. The δ-core has much less activity as compared to holoenzyme on various DNA templates. However, sigma factor does stimulate the activity of δ-core enzyme under conditions of RNA synthesis. These observations and the results of others suggest that δ-core enzyme binds initially to specific DNA sites followed by δ release from the core-DNA complex and that the sigma factor binds to the core-DNA complex to initiate RNA synthesis. Thus both δ and sigma factors are required in a sequential fashion for specific transcription to occur in B. subtilis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00274183
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  • 7
    Electronic Resource
    Electronic Resource
    Homology of Saccharomyces cerevisiae ADH4 to an iron-activated alcohol dehydrogenase from Zymomonas mobilis (1987)
    Springer
    Molecular genetics and genomics 209 (1987), S. 374-381 
    Details
    ISSN: 1617-4623
    Keywords: Alcohol dehydrogenase ; Saccharomyces cerevisiae ; Zymomonas mobilis ; Evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Insertion of the transposable element Ty at the ADH4 locus results in increased levels of a new alcohol dehydrogenase (ADH) activity in Saccharomyces cerevisiae. The DNA sequence of this locus has been determined. It contains a long open reading frame which is not homologous to the other ADH isozymes that have been characterized in S. cerevisiae nor does it show obvious homology to Drosophila ADH. The hypothetical ADH does, however, show strong homology to the sequence of an iron-activated ADH from the bacterium Zymomonas mobilis. Thus ADH4 appears to encode an ADH structural gene which, along with the Zymomonas enzyme, may define a new family of alcohol dehydrogenases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00329668
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  • 8
    Electronic Resource
    Electronic Resource
    Analysis of mutations affecting Ty-mediated gene expression in Saccharomyces cerevisiae (1981)
    Springer
    Molecular genetics and genomics 182 (1981), S. 159-163 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Yeast translocatable, Ty, elements can cause constitutive synthesis of the glucose-repressible alcohol dehydrogenase (ADHII) when inserted upstream from the 5′ end of the structural gene, ADR2. These insertion mutations, ADR3 c, are unstable and give rise to secondary ADHII− mutations. The majority of such mutants, adr3, can be attributed to excision of the insertion sequence, leaving behind a single copy of the δ-sequence which occurs as a direct repeat at the ends of the Ty elements. A few adr3 mutants appear to be generated by DNA-rearrangements in the vicinity of the Ty insertion. The occurrence of recessive mutants, tye, which are unlinked to ADR2 indicates that the constitutive expression of ADR2 caused by the Ty insertions requires the function of trans-acting genes. These results support the idea that regulation of Ty-linked ADR2 is actively mediated by the insertion sequence and is probably not due to a mere disruption of the wild-type controlling site.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00422784
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  • 9
    Electronic Resource
    Electronic Resource
    Sigma factor is not released during transcription in Bacillus subtilis (1979)
    Springer
    Molecular genetics and genomics 174 (1979), S. 47-52 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The relationship between sigma (σ) and delta (δ) factors of Bacillus subtilis RNA polymerase has been analyzed during initiation of RNA synthesis. When core enzyme (E) containing delta factor (Eδ) binds to DNA, the delta factor is released with the formation of an E-DNA complex. The addition of sigma to the E-DNA complex results in the formation of a stable Eσ-DNA complex which can synthesize RNA upon addition of nucleoside triphosphates. Sigma factor, significantly, is not released from the core during RNA synthesis. These results suggest that delta and sigma factors can act sequentially during initiation of RNA synthesis with delta acting as a DNA recognition factor and sigma acting as an initiation factor. The results do not preclude the possibility that Eσ can initiate RNA synthesis correctly since Eσ alone can bind to DNA and initiate RNA synthesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00433304
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  • 10
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    Response of soybean cyst nematode (Heterodera glycines) and root-knot nematodes (Meloidogyne spp.) to gradients of pH and inorganic salts (2020)
    Springer
    Details
    Publication Date: 2020-08-25
    Print ISSN: 0032-079X
    Electronic ISSN: 1573-5036
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Springer
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