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  • 1
    Electronic Resource
    Electronic Resource
    Analysis of hybrids of Candida albicans formed by protoplast fusion (1994)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 115 (1994), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract A number of hybrids obtained by fusion of protoplasts of complementary auxotrophic strains of Candida albicans were analysed for growth rates, cell volume, DNA content, stability and adherence to human buccal epithelial cells. SDS-PAGE analysis of the cell wall proteins indicated that the hybrid cell walls contained many more of the proteins associated with one parent than the other. The adherence values of the hybrids were closest to the parent with which they shared most cell wall proteins and it is suggested that the hybrids contain the genome of this parent along with one or more of the chromosomes of the other.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1994.tb06617.x
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  • 2
    Electronic Resource
    Electronic Resource
    Multiple transformation of Saccharomyces cerevisiae by protoplast fusion (1992)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 99 (1992), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract A technique for the multiple transformation of yeast by protoplast fusion is described. This involved the PEG-induced fusion of protoplasts from cells which had been treated with chromosome-fragmenting agents (in this case cupferron and hydroxylamine) with protoplasts of triply auxotrophic cells. The recovery of transformants was increased significantly if one of the amino acid requirements of the recipient strain was included in the selection medium. Transformants isolated on supplemented media remained auxotrophic for that requirement. Prototrophic, uninucleate transformants had a DNA content and cellular volume similar to that of the parental strains. Possible mechanisms of gene transfer are discussed. This technique offers the possibility of transferring desirable characteristics from one yeast strain to another without altering the ploidy level of the recipient strain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1992.tb05565.x
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  • 3
    Electronic Resource
    Electronic Resource
    European Passenger Services Limited - assessment centres for recruitment and development (1996)
    Bingley : Emerald
    Career development international 1 (1996), S. 12-16 
    Details
    ISSN: 1362-0436
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Economics
    Notes: Describes the evolution of European Passenger Services from its creation as a subsidiary of British Rail, through direct ownership by Government, to impending private sector ownership. Explains the international business in which EPS operates. The main thrust of the article describes the approach to recruitment and selection of a new workforce, using competence-based assessment centres. Describes two assessment centres in detail. Conclusions include evaluation of planned use of the competence framework for career development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1108/13620439610129977
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  • 4
    Electronic Resource
    Electronic Resource
    Protoplast fusion in a petite-negative yeast, Kluyveromyces lactis (1980)
    Springer
    Current genetics 2 (1980), S. 87-93 
    Details
    ISSN: 1432-0983
    Keywords: Protoplast fusion ; Kluyveromyces ; Hybrid characterization ; Stable Diploids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The technique of protoplast fusion has been applied to the problem of unstable diploidy in the yeast Kluyveromyces lactis. By protoplast fusion between heterothallic strains of like mating-type, sporulation-deficient hybrids can be obtained. Biochemical, cytological, and genetical characterisation of these hybrids suggests that the majority of fusion products are diploid. Sporulating hybrids can be constructed by protoplast fusion between homothallic strains. Tetrad analysis of these hybrids demonstrates conclusively the diploid nature of fusion products.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00445699
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  • 5
    Electronic Resource
    Electronic Resource
    Isolation and characterization of respiration-deficient mutants from the pathogenic yeastCandida albicans (1992)
    Springer
    Antonie van Leeuwenhoek 61 (1992), S. 207-219 
    Details
    ISSN: 1572-9699
    Keywords: Candida ; mitochondria ; mutants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The isolation of several respiration deficient mutants of the pathogenic yeastCandida albicans is described. These show greatly reduced respiration rates, loss of cytochromesaa 3 andb, and reduced growth rates. All of the mutants had lost the ability to assimilate a wide range of carbon sources. Ultrastructural studies showed reduced development of mitochondrial cristae in the mutants. The mutants can be divided into three classes depending on their respiration responses to the addition of cyanide.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00584227
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  • 6
    Electronic Resource
    Electronic Resource
    The effect of mycophenolic acid on the cell cycle ofCandida albicans (1990)
    Springer
    Mycopathologia 111 (1990), S. 165-168 
    Details
    ISSN: 1573-0832
    Keywords: Candida albicans ; mycophenolic acid ; cell cycle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Mycophenolic acid inhibited the growth ofCandida albicans. Cultures exposed to a concentration of 8.4 μg ml−1 mycophenolic acid were found to exhibit cell cycle arrest with two or more buds. Nuclear staining revealed that these were nucleate implying a possible defect in cytokinesis. The results are discussed in relation to the possible mode of action of mycophenolic acid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02282799
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  • 7
    Electronic Resource
    Electronic Resource
    Effect of sub-inhibitory concentration of chlorhexidine on lipid and sterol composition of shape Candida albicans (1997)
    Springer
    Mycopathologia 140 (1997), S. 69-76 
    Details
    ISSN: 1573-0832
    Keywords: Chlorhexidine ; phospholipids ; fatty acid ; ergosterol Candida albicans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The effect of a sub-inhibitory concentration of chlorhexidine on lipid and sterol composition of Candida albicans was investigated. The total lipid content of this yeast grown in the presence of chlorhexidine was reduced whilst the total sterol content was increased compared with control-grown cells. Lipids and sterol analyses of this yeast grown in the presence and absence of chlorhexidine are presented. Chlorhexidine-grown yeast had a higher level of phosphatidylethanolamine, phosphatidylcholine and monogalactosyldiacylglycerol. Lower proportions of phosphatidylinositol plus phosphatidylserine, phosphatidic acid and cardiolipin were found in C. albicans grown in the presence of the drug when compared with control-grown yeast. The major fatty acids in control-grown cells were C16 and C18. Drug grown-cells had higher proportions of palmitic acid (16 : 0) and stearic acid (18 : 0), but lower proportions of palmitoleic acid (16 : 1) and oleic acid (18 : 1). Chlorhexidine also decreased the unsaturated-to-saturated fatty acid ratio, while the C16/C18 ratios increased compared to control-grown cells. Differences in the fatty acid composition of major phospholipids and neutral lipids between drug and control-grown yeast were also detected. Sterol analysis of control-grown cells showed that the major sterol present was ergosterol (55.4% wt). A significant increase in ergosterol and obtusifoliol was observed in chlorhexidine-treated cells and a significant decrease in squalene and lanosterol. Our results suggested that chlorhexidine affected the lipid and sterol composition of C. albicans.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006852207366
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  • 8
    Electronic Resource
    Electronic Resource
    Respiratory repression and the stability of the mitochondrial genome (1977)
    Springer
    Molecular genetics and genomics 151 (1977), S. 333-342 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The variation in sensitivity of the mitochondrial genome of Saccharomyces cerevisiae to ethidium bromide-induced petite mutation in response to changes in glucose concentration has been studied. Growth in high glucose considerably depressed the mutation rate, whilst small variations are observed in response to step-up or step-down in glucose concentration. Variations in mitochondrial DNA and respiratory activity during the mutagenic process are described. Effects of non-metabolizable sugars which repress mitochondrial biogenesis and a number of antimitochondrial drugs have been investigated. The results are discussed in terms of possible mechanisms of modulation of the mutation rates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00268798
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  • 9
    Electronic Resource
    Electronic Resource
    The use of protoplast fusion in demonstrating chromosomal and mitochondrial inheritance of respiratory-deficiency in Kluyveromyces lactis, a petite-negative yeast (1978)
    Springer
    Molecular genetics and genomics 159 (1978), S. 297-299 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Protoplast fusion has provided us with a means of genetical analysis in respiratory-deficient mutants of the petite-negative yeast, Kluyveromyces lactis. Demonstration of mitochondrial-inheritance in respiratory-deficient mutants of petite-negative yeasts has not previously been possible due to the inviability of petite/microcolony cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00268266
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  • 10
    Electronic Resource
    Electronic Resource
    Isolation of metabolically active Petite mutants of Kluyveromyces lactis, a petite-negative yeast (1977)
    Springer
    Molecular genetics and genomics 156 (1977), S. 93-98 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Conditions under which complete cultures of the petite-negative yeast Kluyveromyces lactis can be converted to metabolically active petite mutants have been found. These mutants, which lack mitochondrial protein synthesis have been shown to be metabolically active by their ability to exclude the dye trypan blue. They appear to possess a functional protein synthesising system, which is sensitive to the inhibitor trichodermin. However, on transfer to solid nutrient medium, these mutants fail to grow normally, and give rise to microcolonies composed of up to a thousand cells. These colonies autolyse after several days.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00272257
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