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  • 1
    Publication Date: 2018-10-22
    Description: An International Ocean Discovery Program (IODP) workshop was held at Sydney University, Australia, from 13 to 16 June 2017 and was attended by 97 scientists from 12 countries. The aim of the workshop was to investigate future drilling opportunities in the eastern Indian Ocean, southwestern Pacific Ocean, and the Indian and Pacific sectors of the Southern Ocean. The overlying regional sedimentary strata are underexplored relative to their Northern Hemisphere counterparts, and thus the role of the Southern Hemisphere in past global environmental change is poorly constrained. A total of 23 proposal ideas were discussed, with 12 of these deemed mature enough for active proposal development or awaiting scheduled site survey cruises. Of the remaining 11 proposals, key regions were identified where fundamental hypotheses are testable by drilling, but either site surveys are required or hypotheses need further development. Refinements are anticipated based upon regional IODP drilling in 2017/2018, analysis of recently collected site survey data, and the development of site survey proposals. We hope and expect that this workshop will lead to a new phase of scientific ocean drilling in the Australasian region in the early 2020s.
    Repository Name: EPIC Alfred Wegener Institut
    Type: Article , NonPeerReviewed
    Format: application/pdf
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  • 2
    Publication Date: 2018-12-01
    Description: The Long Duration Exposure Facility (LDEF) satellite retrieved this year by the U.S. Space Shuttle offers spacecraft designers an unprecedented opportunity to examine synergistic, long-term space environmental effects on systems and materials. This paper discusses the strategy for data development and the role its implementation will play in the design of the Space Station Freedom. Examples are provided from three key areas (environments definition, protection of external surfaces, and verification of system components) to illustrate LDEF's potential contribution.
    Keywords: SPACECRAFT DESIGN, TESTING AND PERFORMANCE
    Type: IAF PAPER 90-080
    Format: text
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Planta 98 (1971), S. 279-284 
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Germinating jack bean cotyledons liberated 14CO2 when fed 14C-guanidoxy-canavanine but did not accumulate any 14C-compounds other than the applied canavanine. This suggested that the canavanine was being degraded by the action of canavanase to canaline and urea, the urea then being converted to ammonia and carbon dioxide by the action of urease. Hydroxyurea and acetohydroxamic acid (both inhibitors of urease activity) strongly inhibited the liberation of 14CO2 from 14C-guanidoxy-canavanine by the cotyledons but neither compound induced the accumulation of 14C-urea within the tissues. This inhibitory action of hydroxyurea on 14CO2 output was thought to be due at least in part, to this inhibition of canavanase activity.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-0778
    Keywords: colorimetric assay ; hybridoma cells ; neutral red ; viable cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A colorimetric assay utilising Neutral Red (C.I. 50040), a nuclear stain, was developed to determine the cellular viability of hybridoma cells in microtitre plates. A linear correlation (r=0.99) was found to exist between the uptake of Neutral Red by viable cells and the viable cell count determined by Trypan blue exclusion test. The linearity stretched over the range of cell concentrations normal in batch cultures (2–30×104/0.2 ml) with as little as ±6% intra-plate well-to-well variation and ±10.2% inter-assay variation. Microscopical examinations of viable hybridoma cells stained with Neutral Red showed that it was located in the nucleus. The possble bifunctional activity of the Neutral Red assay as a test for cellular viability and estimating the DNA content of hybridoma cells is discussed along with its application in a drug screening programme.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 19 (1977), S. 1735-1738 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: This paper describes the scale-up from 0.1 to 100 liter of the unit process based on 3-mm-diameter glass spheres for the growth of BHK monolayer cells. The production of four strains of FMD virus at the 0.1-, 10-, and 100-liter scales was examined. Cell growth was estimated from measurements of the concentration of glucose in the growth medium, while the release of virus was inferred from measuring the concentration of LDH in the culture supernatant fluid. The yields of virus at 0.1-, 1-, and 10-liter scales were similar but that from the 100-liter version was somewhat lower. The reason for this lower yield and the method used to overcome it over outlined.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: In view of the advantages which are associated with the use of the BHK monolayer cell for the production of foot-and-mouth disease (FMD) virus, a unit system using glass spheres was developed to grow BHK monolayer cells and to test the susceptibility of such cells to FMD virus. The yield of cells and their susceptibility compares favorably with BHK monolayer cells which have been grown in Roux bottles.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Methods are described which make possible the production of foot-and-mouth disease (FMD) virus from BHK 21 C13 monolayer cells which have been grown on the surface of serum coated DEAE Sephadex A50 beads. The yield of cells and their susceptibility to infection by FMD virus are equivalent to conventional Roux monolayer systems. The potential for the commercial application of the DEAE Sephadex A50 system is discussed in relation to other unit process monolayer systems and in particular to the system in which cells are cultured in a deep bed of small glass spheres.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1741-2765
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Experimental and finite-element analyses are presented for the anisotropic states of stress, strain and fracture of a glass-epoxy plate containing a circular hole and subjected to uniaxial tension. Strains were experimentally measured using foil gages, moiré and birefringent coating. Stresses are computed in the linear range from the measured strains. While the hole reduces the plate strength by a factor of two, the maximum tensile strain at fracture is greater than the ultimate strain in a plate without a hole. Fracture consists of crack initiation at the hole boundary but off the horizontal axis. Away from the hole, failure is accompanied by considerable delamination. Discontinuous crack propagation is present.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1741-2765
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Strain distributions to failure, tensile and compressive strain-concentration factors, and strength-reduction factors were determined for glass-, boron-, and graphite-epoxy plates with holes loaded in tension. Strain gages, photoelastic coatings and moiré techniques were used. Ten variations of layup and stacking sequence were studied. The boron-epoxy composite was found to be the stiffest and strongest of the three. The graphite laminate with the highest stress concentration and the most linear strain response exhibited the highest strength-reduction factor. In all cases, the maximum strain at failure on the hole boundary was higher than the ultimate tensile-coupon strain. In general, it was found that, the higher the stress-concentration factor, the higher the strength-reduction factor. Thus, the [0/90/0/90]s layup with a stress-concentration factor of 4.82 had a strength-reduction factor of 3.18. At the other extreme, the most flexible layup [±45/±45]s with the lowest stress-concentration factor of 2.06 had the lowest strength-reduction factor of 1.10. Stacking sequences associated with the tensile interlaminar normal stress or high interlaminar shear stress near the boundary, resulted in laminates 10 to 20 percent weaker than corresponding alternate stacking sequences. Furthermore, it was found that stacking-sequence variations can alter the mode of failure from catastrophic to noncatastrophic.
    Type of Medium: Electronic Resource
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