ISSN:
1432-0614
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Summary In immobilized cell preparations growth of cells outside the immobilization matrix as free cells is normally undesirable due to the appearance of cells in the product stream and clogging of such systems. Antibiotics could be used to arrest such free cell growth while allowing the synthesis and excretion of the product into the medium. Chloramphenicol (200 μ/ml) and/or novobiocin (10 μ/ml), when added during the growth of Bacillus subtilis allowed the production and excretion of lysine into the medium. Chloramphenicol at 200 μ/ml effectively arrested free cell growth and hence the lysine being produced was almost entirely due to immobilized cells. Novobiocin on the other hand at concentrations of 100 μ/ml, stopped free cell growth, but also prevented the production of l-lysine. Productivities and yields of lysine were adversely affected by chloramphenicol or novobiocin, probably due to a great decrease in cell viability.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00165876
Permalink