ISSN:
1432-0991
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract A procedure for preparation ofAnaplasma marginale free of erythrocytic antigens is deseribed. The protocol employed heat-stable hemolysin produced byPseudomonas aeruginosa to lyse the erythrocytes and releaseA. marginale. Most of the contaminatng erythrocytic components were solubilized by enzymatic digestion. Final purification was accomplished by differential centrifugation. Electron microscopy and serological criteria established the absence of contaminating host cell antigens.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01567427
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