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  • 1
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Biochemistry 13 (1974), S. 1719-1725 
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Journal of the American Chemical Society 96 (1974), S. 1581-1584 
    ISSN: 1520-5126
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-1432
    Keywords: Protein structure ; Lysozyme ; Structural correspondence ; Divergent evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The three-dimensional structure of goose-type lysozyme (GEWL), determined by x-ray crystallography and refined at high resolution, has similarities to the structures of hen (chicken) eggwhite lysozyme (HEWL) and bacteriophage T4 lysozyme (T4L). The nature of the structural correspondence suggests that all three classes of lysozyme diverged from a common evolutionary precursor, even though their amino acid sequences appear to be unrelated (Grütter et al. 1983). In this paper we make detailed comparisons of goose-type, chicken-type, and phage-type lysozymes. The lysozymes have undergone conformational changes at both the blobal and the local level. As in the globins, there are corresponding α-helices that have rigid-body displacements relative to each other, but in some cases corresponding helices have increased or decreased in length, and in other cases there are helices in one structure that have no counterpart in another. Independent of the overall structural correspondence among the three lysozyme backbones is another, distinct correspondence between a set of three consecutive α-helices in GEWL and three consecutive α-helices in T4L. This structural correspondence could be due, in part, to a common energetically favorable contact between the first and the third helices. There are similarities in the active sites of the three lysozymes, but also one striking difference. Glu 73 (GEWL) spatially corresponds to Glu 35 (HEWL) and to Glu 11 (T4L). On the other hand, there are two aspartates in the GEWL active site, Asp 86 and Asp 97, neither of which corresponds exactly to Asp 52 (HEWL) or Asp 20 (T4L). (The discrepancy in the location of the carboxyl groups is about 10 Å for Asp 86 and 4 Å for Asp 97.) This lack of structural correspondence may reflect some differences in the mechanisms of action of three lysozymes. When the amino acid sequences of the three lysozyme types are aligned according to their structural correspondence, there is still no apparent relationship between the sequences except for possible weak matching in the vicinity of the active sites.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    [S.l.] : International Union of Crystallography (IUCr)
    Acta crystallographica 37 (1981), S. 701-710 
    ISSN: 1600-5724
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: An oscillation data collection system for protein crystallography is described. The system consists of a modified Enraf-Nonius precession camera with cylindrical cassette, and stepping motor driven by a flexible microprocessor control system. The X-ray source is graphite-monochromatized radiation from an Elliott GX-21 rotating-anode generator run at 5.5 kW on a focal spot measuring 0.5 × 5.0 mm. The potential advantages of using a relatively large focal spot in conjunction with a graphite monochromator are discussed. Conditions for optimum collimation and X-ray intensity are considered, and it is shown that appropriately designed collimators with adjustable apertures can have substantial advantages over commercially available pinhole collimators. The oscillation films are processed by a procedure based on that of Rossmann [J. Appl. Cryst. (1979), 12, 225-238]. Determination of the initial alignment of the film is facilitated by a pair of reference pins incorporated in the cylindrical cassette. These pins ensure that the position of the film in the cassette is known, and avoid the need for fiducial marks. The crystal alignment and film measurement technique is fully automatic, requiring no prior input other than the approximate starting orientation of the crystal, the approximate unit-cell dimensions, and the angular oscillation range. An alternative method for the determination of crystal orientation is proposed which has been found to be somewhat superior to that of Rossmann, especially for smaller unit cells.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    [S.l.] : International Union of Crystallography (IUCr)
    Acta crystallographica 32 (1976), S. 349-350 
    ISSN: 1600-5724
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: A method is proposed by which a protein model with acceptable stereochemistry can be fitted to a set of atomic coordinates. By expressing all constraints in terms of distances between pairs of atoms it is possible to enforce any desired stereochemistry in a physically realistic manner, and at the same time to substantially reduce computing time. Application of the technique to thermolysin is described. The method has been independently developed and applied to insulin by E. J. Dodson, N. W. Isaacs & J. S. Rollett [Acta Cryst. (1976). A32, 311-315].
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Ground water 2 (1964), S. 0 
    ISSN: 1745-6584
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geosciences
    Notes: Approximately 4 pounds of refuse per capita per day are produced in the United States. The term refuse, as used here, refers to the useless, unused, unwanted, or discarded solid waste materials resulting from normal community activities; refuse includes such materials as garbage, rubbish, ashes, street refuse, dead animals, and solid industrial wastes (Anon., 1958). Thus, every day our urban population produces over 400,000,000 pounds of refuse that must be disposed of by dumping on land, grinding and disposal with sewage, incineration or that must be made reusable by one or more reclamation processes. Over 1400 communities dispose of their refuse by sanitary landfill techniques, i.e., compaction and covering with compacted earth on suitable land by use of mechanical equipment such as crawler type tractors (Figure 1). Many thousands more dispose of this material in open dumps on land without the degree of sanitary control recommended by health agencies (Figure 2).Wherever refuse is deposited on land, the potential impact on surface waters or subterranean aquifers may be significant. This can be better appreciated when one considers, for example, that ordinary community refuse may have a 5-day BOD of 14,000 to 180,000 ppm and an alkalinity (to MO as CaCO3) of 2600 to more than 23,000 ppm, as shown in Table 1 (Anon., 1952a). In one study bacteriological examination of landfill material showed an average of 740,000 coliforms per gram of refuse. The leachate from a landfill has been found to have a 5-day BOD from 6 to more than 7000 ppm (Carpenter and Setter, 1940; McDermott, 1950).The question is, of course, what does this mean translated into terms of potential ground-water pollution? And further, when this potential is known, what then are the practicalities involved in present disposal practices and their implications with respect to the development of existing and future ground-water pollution problems?
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Annals of Physics 77 (1973), S. 250-278 
    ISSN: 0003-4916
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Physics
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 68 (1990), S. 2589-2598 
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: We describe in detail the design and performance of a multikilowatt Pockels cell for use in high average power laser systems. The Pockels cell is a gas-cooled, transversely excited electro-optic switch based on KD*P as the electro-optic material. Matched pairs of crystals with different crystallographic orientations are used to make the switch performance insensitive to the operating point and ambient temperature. Excellent switching performance and low-wave-front distortion have been achieved at average power levels in excess of 1 kW.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    College Park, Md. : American Institute of Physics (AIP)
    Journal of Mathematical Physics 26 (1985), S. 18-20 
    ISSN: 1089-7658
    Source: AIP Digital Archive
    Topics: Mathematics , Physics
    Notes: Unitary irreps of ∼(SL(3,R)) which contain a representation D j of SU(2) at most once are known to exist with SU(2) content j=k+2n (n=0,1,2,.. and k=0, 1/2 ,1). Güler, in a recent paper, claims that there also exist multiplicity-free unitary representations with SU(2) content j=k0, k0+1, k0+2,..., for k0〉3. We show that such representations do not exist for k0〉1.
    Type of Medium: Electronic Resource
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