ISSN:
1040-0397
Keywords:
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
An amperometric flow injection system with an immobilized enzyme reactor that gives responses amplified by substrate recycling was employed for the highly sensitive determination of L-glutamate. An L-glutamate oxidase/glutamic-pyruvic transaminase coimmobilized reactor was incorporated at fixed positions in a flow system. In the presence of sufficient amounts of L-alanine and oxygen in the carrier solution, L-glutamate was repeatedly converted to 2-oxoglutarate by L-glutamate oxidase, and the 2-oxoglutarate produced was subsequently converted back to L-glutamate by glutamic-pyruvic transaminase during the passage through the enzyme reactor. As a result of this recycling reaction, a large amount of hydrogen peroxide was produced in the reactor and could be detected amperometrically at a downstream platinum detector. L-Glutamate was determined with a 20- to 30-fold increase in sensitivity compared to the unamplified responses. The detection limit was 1 pmol of L-glutamate.
Additional Material:
3 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elan.1140020711
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