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  • 1
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    PANGAEA
    In:  Supplement to: Scott, Rebecca; Biastoch, Arne; Agamboue, Pierre D; Bayer, Till; Boussamba, Francois L; Formia, Angela; Godley, Brendan J; Mabert, Brice D K; Manfoumbi, Jean C; Schwarzkopf, Franziska; Sounguet, Guy-Philippe; Wagner, Patrick; Witt, Matthew J (2017): Spatio-temporal variation in ocean current-driven hatchling dispersion: Implications for the world's largest leatherback sea turtle nesting region. Diversity and Distributions, https://doi.org/10.1111/ddi.12554
    Publication Date: 2019-02-12
    Description: This data set describes the location of virtual floats representing turtle hatchlings throughout 60 modeled years. Floats were constrained to remain within depths of 0-6 m due to the positive buoyancy of hatchlings. Floats were first assigned to one of 20,000 random release locations within a large release area 125-400 km offshore from nesting beaches throughout the Republic/Democratic Republic of the Congo, Gabon and Equatorial Guinea spanning latitudes of c. 6°S to 3.5°N. For each month over the 4-month long hatching season (January-April), each of the 20,000 floats was assigned a random release day and drift simulations ran every year during the period 1960-2007 resulting in drift trajectories of approx. 4 million virtual floats. See Scott et al., 2017, Spatio-temporal variation in ocean current-driven hatchling dispersion: Implications for the world's largest leatherback sea turtle nesting region. Diversity Distrib, http://dx.doi.org/10.1111%2Fddi.12554 for details as to the model parameters. Each data set consists of data on the float ID (number 1,2,3 etc..) and its trajectory attributes (latitude/longitude) at each time step. Data are also provided on the temperature, salinity and density of the float at its respective position/time step. Data sets are sorted by float release date, and contain one data file for each year. Each data file has 11 columns, which contain the following data: float id, longitude, latitude, depth, time step, temperature, salinity, density, no time steps since start, distance to start point, bearing from start point
    Type: Dataset
    Format: text/tab-separated-values, 60 data points
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  • 2
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The stx genes of many Shiga toxin-encoding Escherichia coli (STEC) strains are encoded by prophages of the λ bacteriophage family. In the genome of the Stx1-encoding phage H-19B, the stx1AB genes are found ≈ 1 kb downstream of the late phage promoter, pR′, but are known to be regulated by the associated iron-regulated promoter, pStx1. Growth of H-19B lysogens in low iron concentrations or in conditions that induce the prophage results in increased Stx1 production. Although the mechanism by which low iron concentration induces Stx1 production is well understood, the mechanisms by which phage induction enhances toxin production have not been extensively characterized. The studies reported here identify the factors that contribute to Stx1 production after induction of the H-19B prophage. We found that replication of the phage genome, with the associated increase in stx1AB copy number, is the most quantitatively important mechanism by which H-19B induction increases Stx1 production. Three promoters are shown to be involved in stx1AB transcription after phage induction, the iron-regulated pStx1 and the phage-regulated pR and pR′ promoters, the relative importance of which varies with environmental conditions. Late phage transcription initiating at the pR′ promoter, contrary to previous findings in the related Stx2-encoding phage φ361, was found to be unnecessary for high-level Stx1 production after phage induction. Finally, we present evidence that phage-mediated lysis regulates the quantity of Stx1 produced by determining the duration of Stx1 accumulation and provides a mechanism for Stx1 release. By amplifying stx1AB copy number, regulating stx1AB transcription and allowing for Stx1 release, the biology of the Stx-encoding phages contributes greatly to the production of Stx, the principal virulence factor of STEC.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-7357
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The normal state resistivity of high temperature superconductors can be probed in the region below T c by suppressing the superconducting state in high magnetic fields. Here we present the normal state properties of YBa 2 Cu 3 O x thin films in the underdoped regime and the normal state resistance of La 2-x Sr x CuO 4 thin films under epitaxial strain, measured below T c by applying pulsed fields up to 60 T. We interpret these data in terms of the recently proposed 1D quantum transport model with the 1D paths corresponding to the charge stripes.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1662-9752
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Synthetic diamond is regarded as a promising material for biosensors: it forms a stable platform for genetic assays and its biocompatibility opens the possibility for in vivo sensing. In this study the use of a thymidine linker for covalent DNA attachment was evaluated. Contact angle measurements provided a qualitative test of the initially oxidized surface. X-ray photoemission spectroscopy was used for further analysis of the oxides and for monitoring the effect of subsequent chemical treatments. The presence of FITC-labelled DNA was confirmed by confocal fluorescence microscopy. Enzyme linked immunosorbent assays indicated that this DNA was merely adsorbed on the diamond surface instead of covalently bound
    Type of Medium: Electronic Resource
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  • 5
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    In:  (Bachelor thesis), Christian-Albrechts-Universität Kiel, Kiel, Germany, 39 pp
    Publication Date: 2019-11-29
    Keywords: Course of study: BSc Physics of the Earth System
    Type: Thesis , NonPeerReviewed
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  • 6
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    In:  [Talk] In: DRAKKAR 2018 Annual Workshop, 22.-24.01.2018, Grenoble, France .
    Publication Date: 2019-11-29
    Description: Sea surface height (SSH) variability in the tropical Pacific is determined by surface fluxes of which momentum flux is the most important one. The new atmospheric forcing data set JRA-55-do (Tsujino, H. et al. (submitted)) offers the chance of an improved representation of tropical SSH variability. We present a comparison of SSH variability of the tropical Pacific in two global ORCA025 (Nemo3.6) configurations, forced with COREv2 and JRA-55-do atmospheric datasets. While the amplitude of SSH variability in COREv2-runs is in close agreement with altimeter observations, a reduced interannual variability of wind stress in JRA-55-do leads to weaker SSH-variability in the tropical Pacific. A lagged correlation analyses of SSH with climate indices such as ENSO or Southern Oscillation shows a higher agreement between JRA-55-do and altimeter observations than between COREv2 and observations, suggesting an improved representation of the processes that determine SSH variability
    Type: Conference or Workshop Item , NonPeerReviewed
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  • 7
    Publication Date: 2019-11-29
    Description: To model tracer spreading in the Ocean, Lagrangian simulations in an offline framework are a practical and efficient alternative to solving the advective-diffusive tracer equations online. Differences in both approaches raise the question whether both methods are comparable. Lagrangian simulations usually use model output averaged in time, and trajectories are not subject to parameterized subgrid diffusion which is included in the advection-diffusion equations of ocean models. Previous studies focused on diffusivity estimates in idealized models but could show that both methods yield similar results as long as the deformations scale dynamics are resolved and a sufficient amount of floats is used. This study compares the spreading of an Eulerian tracer simulated online and a cloud of Lagrangian particles simulated offline with velocities from the same model. We use a global, eddy-resolving ocean model featuring 1/20° horizontal resolution in the Agulhas region around South Africa. Tracer and particles were released at one time step in the Cape Basin and below the mixed layer at a depth of 160 m and integrated for 3 years. Large-scale diagnostics, like mean pathways of floats and tracer, are almost identical and 1D-horizontal distributions show no signigicant differences. Differences in vertical distributions, seen in a reduced vertical spreading and downward displacement of particles, are due to the combined effect of unresolved sub-daily variability of the vertical velocities and the spatial variation of vertical diffusivity. This, in turn, has a small impact on the horizontal spreading behavior. The estimates of eddy diffusivity from particles and tracer yield comparable results of about 4048 m^2/s in the Cape Basin.
    Type: Conference or Workshop Item , NonPeerReviewed
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  • 8
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    Unknown
    AMS (American Meteorological Society)
    In:  Journal of Physical Oceanography, 49 (5). pp. 1141-1157.
    Publication Date: 2019-11-29
    Description: To model tracer spreading in the ocean, Lagrangian simulations in an offline framework are a practical and efficient alternative to solving the advective–diffusive tracer equations online. Differences in both approaches raise the question of whether both methods are comparable. Lagrangian simulations usually use model output averaged in time, and trajectories are not subject to parameterized subgrid diffusion, which is included in the advection–diffusion equations of ocean models. Previous studies focused on diffusivity estimates in idealized models but could show that both methods yield similar results as long as the deformations-scale dynamics are resolved and a sufficient amount of Lagrangian particles is used. This study compares spreading of an Eulerian tracer simulated online and a cloud of Lagrangian particles simulated offline with velocities from the same ocean model. We use a global, eddy-resolving ocean model featuring 1/20° horizontal resolution in the Agulhas region around South Africa. Tracer and particles were released at one time step in the Cape Basin and below the mixed layer and integrated for 3 years. Large-scale diagnostics, like mean pathways of floats and tracer, are almost identical and 1D horizontal distributions show no significant differences. Differences in vertical distributions, seen in a reduced vertical spreading and downward displacement of particles, are due to the combined effect of unresolved subdaily variability of the vertical velocities and the spatial variation of vertical diffusivity. This, in turn, has a small impact on the horizontal spreading behavior. The estimates of eddy diffusivity from particles and tracer yield comparable results of about 4000 m2 s−1 in the Cape Basin.
    Type: Article , PeerReviewed
    Format: text
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  • 9
    Publication Date: 2019-11-29
    Description: Aim: The lives of juvenile leatherback turtles are amongst the most enigmatic of all marine mega-vertebrates. For these cryptic organisms, ocean models provide important insights into their dispersion from natal sites. Here, corroborated by fisheries bycatch data, we simulate spatio-temporal variation in hatchling dispersion patterns over five decades from the World's largest leatherback turtle nesting region. Location: Equatorial Central West Africa (3.5°N to −6°S) spanning the Gulf of Guinea in the North, Gabon and the Republic/Democratic Republic of the Congo in the South. Results: Due to dynamic oceanic conditions at these equatorial latitudes, dispersion scenarios differed significantly: (1) along the north to south gradient of the study region, (2) seasonally and (3) between years. From rookeries to the north of the equator, simulated hatchling retention rates within the Gulf of Guinea were very high (〉99%) after 6 months of drift, whilst south of the equator, retention rates were as low as c. 6% with the majority of simulated hatchlings dispersing west into the South Atlantic Ocean with the South Equatorial Current. Seasonal dispersion variability was driven by wind changes arising from the yearly north/southward migration of the intertropical convergence zone resulting in the increasing westerly dispersion of hatchlings throughout the hatching season. Annual variability in wind stress drove a long-term trend for decreased retention within the Gulf of Guinea and increased westerly dispersion into habitats in the South Atlantic Ocean. Main conclusions: Shifts in dispersion habitats arising from spatio-temporal oceanic variability expose hatchlings to different environments and threats that will influence important life history attributes such as juvenile growth/survival rates; anticipated to impact the population dynamics and size/age structure of populations into adulthood. The impacts of local and dynamic oceanic conditions thus require careful considerations, such as subregional management, when managing marine populations of conservation concern.
    Type: Article , PeerReviewed
    Format: text
    Format: text
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  • 10
    Publication Date: 2019
    Description: Monitoring the cellular metabolism of bacteria in (bio)fermentation processes is crucial to control and steer them, and to prevent undesired disturbances linked to metabolically inactive microorganisms. In this context, cell-based biosensors can play an important role to improve the quality and increase the yield of such processes. This work describes the simultaneous analysis of the metabolic behavior of three different types of bacteria by means of a differential light-addressable potentiometric sensor (LAPS) set-up. The study includes Lactobacillus brevis, Corynebacterium glutamicum, and Escherichia coli, which are often applied in fermentation processes in bioreactors. Differential measurements were carried out to compensate undesirable influences such as sensor signal drift, and pH value variation during the measurements. Furthermore, calibration curves of the cellular metabolism were established as a function of the glucose concentration or cell number variation with all three model microorganisms. In this context, simultaneous (bio)sensing with the multi-organism LAPS-based set-up can open new possibilities for a cost-effective, rapid detection of the extracellular acidification of bacteria on a single sensor chip. It can be applied to evaluate the metabolic response of bacteria populations in a (bio)fermentation process, for instance, in the biogas fermentation process.
    Electronic ISSN: 1424-8220
    Topics: Chemistry and Pharmacology , Electrical Engineering, Measurement and Control Technology
    Published by MDPI
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