ISSN:
1612-1112
Keywords:
Column liquid chromatography
;
Ligand-exchange chromatography
;
Chiral stationary phases
;
Enantiomer separation
;
Amino acids
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary Chiral stationary phases (CSPs) were synthesized by reaction of aminopropylsilica (APS) with chiral monochloro-s-triazines (MCTs). MCTs were obtained by reaction of 2,4,6-trichloro-s-triazine (cyanuric chloride) with one equivalent of methanol and, subsequently, with one equivalent of L-prolinetert butyl ester (H-Pro-OtBu) orN-tert butyloxycarbonyl-L-lysinetert butyl ester (Boc-Lys-OtBu). End-capping of unreacted amino groups of APS with acetic anhydride, followed by trifluoroacetolytic cleavage of the protecting groups of amino acids (AAs), afforded two chiral stationary phases bearing either L-proline (CSP-3) or L-lysine (CSP-4) as chiral selector. Using ligand-exchange chromatography matography with addition of Cu2+ to the mobile phase, enantiomers of free DL-AAs and a fewN-(2,4-dinitrophenyl)-DL-AAs were separated on CSP-3, whereasN-(dansyl)-DL-AAs were separated on CSP-4.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02467446
Permalink