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  • 1
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    Positional cloning of a gene for nematode resistance in sugar beet (1997)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1997-02-07
    Description: The Hs1(pro-1) locus confers resistance to the beet cyst nematode (Heterodera schachtii Schmidt), a major pest in the cultivation of sugar beet (Beta vulgaris L.). The Hs1(pro-1) gene was cloned with the use of genome-specific satellite markers and chromosomal break-point analysis. Expression of the corresponding complementary DNA in a susceptible sugar beet conferred resistance to infection with the beet cyst nematode. The native Hs1(pro-1) gene, expressed in roots, encodes a 282-amino acid protein with imperfect leucine-rich repeats and a putative membrane-spanning segment, features similar to those of disease resistance genes previously cloned from higher plants.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Cai, D -- Kleine, M -- Kifle, S -- Harloff, H J -- Sandal, N N -- Marcker, K A -- Klein-Lankhorst, R M -- Salentijn, E M -- Lange, W -- Stiekema, W J -- Wyss, U -- Grundler, F M -- Jung, C -- New York, N.Y. -- Science. 1997 Feb 7;275(5301):832-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institute of Crop Science and Plant Breeding, Christian-Albrechts-University of Kiel, Olshausenstrasse 40, D-24118 Kiel, Germany.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9012350" target="_blank"〉PubMed〈/a〉
    Keywords: Amino Acid Sequence ; Animals ; Cell Membrane/chemistry ; *Cloning, Molecular ; DNA, Complementary/genetics ; *Genes, Plant ; Genetic Complementation Test ; Leucine/chemistry ; Membrane Proteins/chemistry/*genetics/physiology ; Molecular Sequence Data ; Nematoda/*pathogenicity ; Plant Diseases/*genetics/parasitology ; *Plant Proteins ; Plant Roots/genetics/parasitology ; Transformation, Genetic ; Vegetables/*genetics/*parasitology
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 2
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    Unknown
    Adaptation of Spodoptera exigua larvae to plant proteinase inhibitors by induction of gut proteinase activity insensitive to inhibition. (1995)
    National Academy of Sciences
    Details
    Publication Date: 1995-08-15
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
    Published by National Academy of Sciences
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  • 3
    Electronic Resource
    Electronic Resource
    Mapping of loci involved in quantitatively inherited resistance to the potato cyst-nematode Globodera rostochiensis pathotype Ro1 (1993)
    Springer
    Theoretical and applied genetics 87 (1993), S. 464-470 
    Details
    ISSN: 1432-2242
    Keywords: Solanum spegazzinii ; Globodera rostochi ensis ; Nematode resistance ; QTL ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We report the identification and mapping of two quantitative trait loci (QTLs) of Solanum spegazzinii BGRC, accession 8218-15, involved in resistance to the potato cyst-nematode Globodera rostochiensis pathotype Ro1, by means of restriction fragment length polymorphisms (RFLPs). For this purpose we crossed a susceptible diploid S. tuberosum with the resistant S. spegazzinii, and tested the F1 population for resistance to the Ro1 pathotype. Since the F1 segregated for the resistance, the S. spegazzinii parent was concluded to be heterozygous at the nematode resistance loci. For the mapping of the resistance loci we made use of RFLP markers segregating for S. spegazzinii alleles in the F1. One hundred and seven RFLP markers were tested in combination with four different restriction enzymes; 29 of these displayed a heterozygous RFLP pattern within S. spegazzinii and were used for mapping. Analysis of variance (ANOVA) was applied to test the association of the RFLP patterns of these markers with nematode resistance. Two QTLs involved in disease resistance to Globodera rostochiensis pathotype Ro1 were identified and mapped to chromosomes 10 and 11 respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00215092
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  • 4
    Electronic Resource
    Electronic Resource
    The impact on biosafety of the phosphinothricin-tolerance transgene in inter-specific B. rapa×B. napus hybrids and their successive backcrosses (1997)
    Springer
    Theoretical and applied genetics 95 (1997), S. 442-450 
    Details
    ISSN: 1432-2242
    Keywords: Key words Biosafety ; Brassica rapa ; Inter-specific hybridization ; Phosphinothricin-tolerance gene ; Transgenic Brassica napus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  There is strong evidence indicating that gene flow from transgenic B. napus into weedy wild relatives is inevitable following commercial release. Research should now focus on the transmission, stability, and impact of transgene expression after the initial hybridization event. The present study investigated the transfer of a phosphinothricin-tolerance transgene by inter-specific hybridization between B. rapa and two transgenic B. napus lines. The expression of the transgene was monitored in the F1 hybrids and in subsequent backcross generations. The transgene was transmitted relatively easily into the F1 hybrids and retained activity. Large differences in the transmission frequency of the transgene were noted between offspring of the two transgenic lines during backcrossing. The most plausible explanation of these results is that the line showing least transmission during backcrossing contains a transgene integrated into a C-genome chromosome. Approximately 10% of offspring retained the tolerant trait in the BC3 and BC4 generations. The implications of these findings for the stable introgression of transgenes carried on one of the chromosomes of the C-genome from B. napus and into B. rapa are briefly discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050581
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  • 5
    Electronic Resource
    Electronic Resource
    Quantitatively-inherited resistance toGlobodera pallida is dominated by one major locus inSolanum spegazzinii (1994)
    Springer
    Theoretical and applied genetics 88 (1994), S. 764-769 
    Details
    ISSN: 1432-2242
    Keywords: Potato cyst-nematode ; Quantitative trait loci RFLP ; Distorted segregation ; Solanum spegazzinii
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A high level of resistance toGlobodera pallida pathotypes Pa2 and Pa3 exists inSolanum spegazzinii, a wild relative of potato (S. tuberosum ssp.tuberosum). Here we report the mapping of loci involved in quantitatively-inherited nematode resistance with the use of RFLPs. One major locus,Gpa, was mapped on chromosome 5 and two minor loci on chromosomes 4 and 7 ofS. spegazzinii. Additionally, the contribution of the susceptible parent to nematode resistance was determined. TheGpa locus was solely responsible for the high resistance level found in the segregating population. However, the RFLP marker closely linked to this resistance locus showed a distorted segregation, with a shortage of plants having the resistance linked allele. Our results indicate that a prediction of the genetic constitution of a quantitative trait based solely on phenotypic observations can lead to erroneous conclusions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01253983
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  • 6
    Electronic Resource
    Electronic Resource
    Long-range organization of a satellite DNA family flanking the beet cyst nematode resistance locus (Hs1) on chromosome-1 of B. patellaris and B. procumbens (1994)
    Springer
    Theoretical and applied genetics 89 (1994), S. 459-466 
    Details
    ISSN: 1432-2242
    Keywords: Molecular markers Beet cyst nematode resistance ; Hs1 Heterodera schachtii Schm ; Satellite DNA Monosomic fragment additions Beta ; Pulsed-field gel electrophoresis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract New members of a satellite DNA family (Sat 121), specific for wild beets of the section Procumbentes of the genus Beta, were isolated. Sequence analysis showed that the members of Sat-121 fall into two distinct classes. The organization of Sat-121 in the vicinity of a beet cyst nematode (Heterodera schachtii Schm.) resistance locus (Hs1) in B. patellaris and B. procumbens was investigated by pulsed-field gel electrophoresis (PFGE) using DNA from a series of resistant monosomic fragment additions, each containing an extra chromosome fragment of B. patellaris chromosome-1 (pat-1) in B. vulgaris. In this way several clusters of Sat-121 flanking the Hs1 pat-1 locus were identified. In nematode resistant diploid introgressions (2n=18), which contain small segments of B. procumbens chromosome-1 (pro-1) in B. vulgaris, only two major Sat-121 clusters were detected near the Hs1 pro-1 locus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225381
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  • 7
    Electronic Resource
    Electronic Resource
    Mapping of QTLs involved in nematode resistance, tuber yield and root development in Solanum sp. (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 463-470 
    Details
    ISSN: 1432-2242
    Keywords: Key words  RFLPs ; Solanum spegazzinii ; Globodera sp. ; Interlocus interaction ; Intralocus interaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract   A backcross population, derived from the cross (S. tuberosum×S. spegazzinii)×S. tuberosum was used to map QTLs involved in nematode resistance, tuber yield and root development. Complete linkage maps were available for the interspecific hybrid parent as well as the S. tuberosum parent, and interval mapping for all traits was performed for both. Additionally, the intra- and inter-locus interactions of the QTLs were examined. The Gro1.2 locus, involved in resistance to G. rostochiensis pathotype Ro1, that was previously mapped in the S. tuberosum×S. spegazzinii F1 population, was located more precisely on chromosome 10. A new resistance locus, Gro1.4, also conferring resistance to G. rostochiensis pathotype Ro1, was found on chromosome 3. Different alleles of this locus originating from both parents contributed to the resistant phenotype, indicating multiallelism at this locus. No interlocus interactions were observed between these two resistance loci. For resistance to G. pallida no QTLs were detected. One minor QTL involved in tuber yield was located on chromosome 4. Two QTLs involved in root development and having large effects were mapped on chromosomes 2 and 6 and an epistatic interaction was found between these two loci.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050150
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  • 8
    Electronic Resource
    Electronic Resource
    Isolation and characterization of RAPD-based markers linked to the beet cyst nematode resistance locus (Hs1 pat-1) on chromosome 1 of B. patellaris (1995)
    Springer
    Theoretical and applied genetics 90 (1995), S. 885-891 
    Details
    ISSN: 1432-2242
    Keywords: Randomly amplified polymorphic DNA (RAPD) ; Deletion mapping ; Sequence Tagged Site (STS) ; Monosomic fragment additions ; Beet cyst nematode resistance ; Heterodera schachtii Schm ; Beta patellaris
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A beet cyst nematode (BCN)-resistant telosomic addition of B. patellaris chromosome 1 in B. vulgaris was used to isolate 6 RAPD markers linked to the BCN resistance locus Hs1 pat-1. Southern analysis showed that the analyzed RAPD products contain either low-, middle or high-repetitive DNA. The relative positions of the random amplified polymorphic DNA (RAPD) markers and of the restriction fragment length polymorphism (RFLP) loci corresponding to the low-repetitive RAPD products were determined by deletion mapping using a panel of seven nematode-resistant B. patellaris chromosome-1 fragment additions. One RAPD marker, OPB11800, was found to be present in two copies on the long arm telosome of B. patellaris chromosome 1. These copies are closely linked to the BCN resistance gene and flank the gene on both sides. On the basis of the nucleotide sequence of OPB11800, sequence-tagged site (STS) primers were developed that amplify specific fragments derived from the two OPB11800 loci. These STS markers can be used in the map-based cloning of the BCN gene, as they define start and finishing points of a chromosomal walk towards the Hs1 pat-1 locus. Two copies of the middle-repetitive OPX21100 marker were mapped in the same interval of the deletion mapping panel as the resistance gene locus and thereby belong to the nearest markers as yet found for the BCN gene in B. patellaris.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222027
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  • 9
    Electronic Resource
    Electronic Resource
    Genetic localisation of transformation competence in diploid potato (1995)
    Springer
    Theoretical and applied genetics 91 (1995), S. 557-562 
    Details
    ISSN: 1432-2242
    Keywords: Solanum tuberosum ; Phytophthora infestans ; Transposable element ; Transformation competence ; Linkage analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In the course of improving diploid potato genotypes for transformation ability, selection for specific components affecting regeneration and transformation was carried out. From a segregating population between two good regenerating clones a selection was made to yield an optimal well-transforming and fertile genotype J92-6400-A16. This plant yielded predominantly diploid transformants and was heterozygous for the gene R1, conferring resistance to Phytophthora infestans. The speed of, and competence for, regeneration and transformation on both sides of the stem explant were improved. A competence factor for tranformation was found to be linked with the R1 locus and a molecular marker on chromosome 5. The male fertility of transformants was frequently decreased to a great extent, whereas female fertility was not so markedly affected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223279
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  • 10
    Electronic Resource
    Electronic Resource
    Mapping of QTLs involved in nematode resistance, tuber yield and root development in Solanum sp. (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 463-470 
    Details
    ISSN: 1432-2242
    Keywords: RFLPs ; Solanum spegazzinii ; Globodera sp ; Interlocus interaction ; Intralocus interaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A backcross population, derived from the cross (S. tuberosumxS. spegazzinii)xS. tuberosum was used to map QTLs involved in nematode resistance, tuber yield and root development. Complete linkage maps were available for the interspecific hybrid parent as well as the S. tuberosum parent, and interval mapping for all traits was performed for both. Additionally, the intra- and inter-locus interactions of the QTLs were examined. The Gro1.2 locus, involved in resistance to G. rostochiensis pathotype Ro1, that was previously mapped in the S. tuberosumxS. spegazzinii F1 population, was located more precisely on chromosome 10. A new resistance locus, Gro1.4, also conferring resistance to G. rostochiensis pathotype Ro1, was found on chromosome 3. Different alleles of this locus originating from both parents contributed to the resistant phenotype, indicating multiallelism at this locus. No interlocus interactions were observed between these two resistance loci. For resistance to G. pallida no QTLs were detected. One minor QTL involved in tuber yield was located on chromosome 4. Two QTLs involved in root development and having large effects were mapped on chromosomes 2 and 6 and an epistatic interaction was found between these two loci.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223694
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