ISSN:
1573-739X
Keywords:
Nitric oxide radical
;
•NO, scavenging
;
Thiol
;
S–nitrosothiol (electrochemical)
;
NO sensing
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract A new method for the quantification of the nitric oxide (•NO) scavenging activity of compounds in aqueous solutions is described using an amperometric •NO sensor. After correction for the spontaneous degradation of •NO, second–order rate kinetics of the scavenging reaction are observe. The rate constant for hemoglobin found with this method is comparable with that found with an established spectrophotometric method. To demonstrate the capability of the method, several sulfur–containing compounds were tested (GSH, GSSG, S–methyl glutathione, N–acetyl cysteine, lipoic acid and dihydrolipoic acid). Of these compounds, only those that contained a thiol group displayed a considerable •NO scavenging activity.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1008601327920
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