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  • 1
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    International Journal of Insect Morphology and Embryology 21 (1992), S. 323-336 
    ISSN: 0020-7322
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Journal of Insect Physiology 38 (1992), S. 569-574 
    ISSN: 0022-1910
    Keywords: Photoperiodism ; Pieris brassicae ; clock ; diapause ; hourglass ; oscillator ; vitamin A
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-8248
    Keywords: Trissolcus basalis ; in vitro rearing ; artificial medium ; Nezara viridula ; egg parasitoid ; insect material ; amino acids balance ; Trissolcus basalis ; élevagein vitro ; milieu oligidique ; Nezara viridula ; parasitoïde oophage ; matériel insecte
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Résumé L'élevagein vitro du parasitoïde oophageTrissolcus basalis a été entrepris à partir d'œufs prélevés dans l'hôte naturelNezara viridula. Plusieurs milieux oligidiques, contenant du “matériel insecte” (hémolymphe de larves deManduca sexta, vitellus d'œufs hôtes) ont été testés. A partir d'un milieu initial à base d'hémolymphe, où le taux de mortalité des œufs était très élevé, nous avons obtenu, en diminuant la teneur en hémolymphe et en augmentant celle en jaune d'œuf de poule, un taux d'éclosion voisin de 70% avec 15% de larves de second stade et 5% de larves de troisième et dernier stade. Des résultats intéressants ont été obtenus dans un milieu dépourvu d'hémolymphe, mais contenant du vitellus d'œufs deN. viridula parasités: 90 à 100% d'éclosion, 25 à 27% de L2 et 8% de L3.
    Notes: Abstract In vitro rearing of the egg parasitoidTrissolcus basalis (WOLL.) from eggs collected on the natural hostNezara viridula (L.) was initiated. Several oligidic diets containing insect material (Manduca sexta hemolymph or host egg content) were tested. Our initial medium with 50% hemolymph induced a high egg mortality, but by decreasing the hemolymph concentration, increasing the hen egg yolk concentration and adding 15% of free amino acids mixture, a hatching rate of 85% of the parasitoid eggs was obtained with 39% reaching the second instar and 33% the third instar. In a medium without hemolymph, but with 18% liquid from parasitized host eggs we obtained 90% to 100% hatching, 25 to 27% reaching the second instar and 8% the third instar. We did not obtain pupation from eggsin vitro, but did get pupae and adults from larvae rearedin vivo to second instar and transfered to anin vitro system.
    Type of Medium: Electronic Resource
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