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  • 1
    Electronic Resource
    Electronic Resource
    Lytic action of strepzymes from micromonospora AS (1965)
    Springer
    Archives of microbiology 51 (1965), S. 156-167 
    Details
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The effect of lytic enzymes of Micromonospora AS on isolated cell walls and intact or heat killed cells of Candida utilis was investigated. Several substances normally used as stabilizers during protoplast formation were tested for their effect on the lytic action of strepzyme M on intact and dead cells: NaCl and KCl markedly inhibited lysis, sucrose only to 40%. Sorbitol and MgSO4 have no inhibitory effect. MgSO4 was selected for further research as it was found to protect the protoplasts. Phosphate buffer pH 6.8 should not be used at concentrations above 0.01 m. When grown submerged in shaking flasks or in pilot fermentation tanks, in liquid medium containing yeast cells and salts, Micromonospora AS gave the highest yield of lytic enzymes. The strepzyme M preparation is thermolabile.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00408723
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  • 2
    Electronic Resource
    Electronic Resource
    Characterization and synthesis regulation of Penicillium italicum 1,6-β-glucanase (1979)
    Springer
    Archives of microbiology 121 (1979), S. 265-270 
    Details
    ISSN: 1432-072X
    Keywords: 1,6-β-Glucanase ; Characterization ; Catabolite repression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The filamentous fungus Penicillium italicum when grown in a synthetic medium, produced and secreted 1,6-β-glucanase into the culture medium. This enzyme has been partially purified by gel filtration. After this step the active fractions were free of 1,3-β-glucanase, α-amylase and β-glucosidase activities. Only four proteins, one associated with the enzyme, were found by polyacrylamide gel electrophoresis under non denaturing conditions. The enzyme behaves as an acidic protein (pI 4.65) with an optimum pH of 5 and an endohydrolytic mode of action. The activity was lost at pHs greater than 7. The enzyme was also found associated with the mycelium. Its synthesis was repressed by glucose or growth-promoting sugars. Derepression in low glucose containing medium required protein synthesis. 8-Hydroxyquinoline, an RNA synthesis inhibitor, added during the derepression period did permit some increase in the specific activity but prevented it when added at the beginning of that period.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425066
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  • 3
    Electronic Resource
    Electronic Resource
    On the effect of glucono-δ-lactone on the yeast Pichia polymorpha (1976)
    Springer
    Archives of microbiology 109 (1976), S. 157-161 
    Details
    ISSN: 1432-072X
    Keywords: Pichia polymorpha ; Streptomyces venezuelae RA ; Penicillium italicum ; β-Glucanase ; Glucono-δ-lactone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Investigation has been made into the action of glucono-δ-lactone on living cells of Pichia polymorpha in relation to the uptake of D-(U-14C) glucose, and the incorporation of (2-14C) uracil and L-(U-14C)-threonine into RNA and protein respectively. Other factors such as the action of glucono-δ-lactone on cell morphology and on enzymic synthesis have also been studied. The action of this compound on β-glucanase has been found to take place in the hydrolytic power and not in the synthesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425129
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  • 4
    Electronic Resource
    Electronic Resource
    An examination of factors affecting the instability of Saccharomyces cerevisiae glucan synthetase in cell free extracts (1984)
    Springer
    Archives of microbiology 137 (1984), S. 209-214 
    Details
    ISSN: 1432-072X
    Keywords: Saccharomyces cerevisiae ; Glucan synthetase ; EDTA ; Magnesium ; Sucrose ; Fluoride
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Yeast β(1–3) glucan synthetase is stimulated and stabilized by EDTA. Sucrose protects the enzyme from selfinactivaton. Preincubation of cell free extracts at low sucrose concentrations indicates a slow transition of the enzyme towards dissociation. Transition kinetics at 30° C and 0° C in the presence and in the absence of sucrose are interpreted assuming that a subunit is thermolabile in the free state and that sucrose increases its stability. Magnesium is deletereous for glucan synthetase in cell-free extracts. Chaotropic agents inactivate glucan synthetase according to their capacity to solubilize and depolymerize biological compounds. Fluoride plays a special role in the activation of glucan synthetase. Its action appears to be dependent on the presence of GTP (or other nucleotides). The role of all these agents on the activity and stability of the enzyme is interpreted in a unified scheme.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00414545
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  • 5
    Electronic Resource
    Electronic Resource
    Synthesis of β-glucanase and other extracellular proteins by cells and protoplasts of the yeast Pichia polymorpha (1975)
    Springer
    Archives of microbiology 105 (1975), S. 335-337 
    Details
    ISSN: 1432-072X
    Keywords: β-Glucanase ; Invertase ; Acid phosphatase ; 2-deoxy-d-glucose
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The synthesis of β-glucanase either by cells or by protoplasts of the yeast Pichia polymorpha has been found to occur in the presence of 2-deoxy-d-glucose in the growth medium. On the other hand, the synthesis of typical extracellular proteins such as invertase and acid phosphatase is strongly affected by the presence of the drug. The degree of inhibition is, however, directly related to the 2-deoxy-d-glucose concentration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00447154
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  • 6
    Electronic Resource
    Electronic Resource
    Electron microscopy of thin sections of Candida utilis: The structure of the cell wall (1965)
    Springer
    Archives of microbiology 50 (1965), S. 103-110 
    Details
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Details of the structure of the Candida utilis cell wall were described. Using intact cells, cell walls, about 0.02 mμ thick, have been resolved into three electron dense layers, each about 700 Å thick, made up of materials of very similar electron opacity. The central and the inner layers of the wall seem to be closely packed forming a slightly more compact structure of somewhat greater electron density. Laminations were observed in some of the inner layers of the sections, particularly in some areas where the cell wall had separated: The possibility of this lamination being associated with the presence of chitin in the framework of the cell wall is discussed. Interesting observations have also been made in the sections of the heat-killed cells confirming the existence of a central electron dense cell wall layer, differing from the inner and the outer ones. Underlying the cell wall is the cytoplasmic membrane, a not too well defined sinous structure with some invaginations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00409122
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  • 7
    Electronic Resource
    Electronic Resource
    Observation of ethylsilylene product in the infrared multiphoton dissociation of ethylsilane (1991)
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 94 (1991), S. 820-821 
    Details
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.460697
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  • 8
    Electronic Resource
    Electronic Resource
    Lytic Activity on Yeast Cell Walls as a Useful Character for the Separation of Streptomyces and Nocardia (1963)
    [s.l.] : Nature Publishing Group
    Nature 198 (1963), S. 911-912 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Various workers have described the results of an investigation into the cell-wall composition of actinomycetes of different types and discussed the application of the chemical analysis of the isolated cell-walls of the representative species of the various genera to the classification of these ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/198911a0
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  • 9
    Electronic Resource
    Electronic Resource
    Lack of Pectic Enzyme Production by Non-Pathogenic Species of Verticillium (1962)
    [s.l.] : Nature Publishing Group
    Nature 195 (1962), S. 1328-1329 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] As a part of the investigations on the lytic activity of a non-pathogenic strain of Verticillium hemileiae on uredospores of various rust2, several species of Verticillium have been tested for ability to produce pectic enzymes in vitro. Pectolytic activity in fungi has generally been found to ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/1951328a0
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  • 10
    Electronic Resource
    Electronic Resource
    A New Method for the Microbiological Assay of Thiamin (Vitamin B 1 ) (1955)
    [s.l.] : Nature Publishing Group
    Nature 176 (1955), S. 465-465 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Several vitamins were tested as growth-promoting factors for T. persicina, namely, thiamin, biotin, riboflavin, pyridoxin, nicotinic acid, inositol and pantothenic acid (this latter in the form of its calcium salt). The vitamins were added aseptically to a synthetic basal medium (autoclaved for 20 ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/176465a0
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