ISSN:
1432-072X
Keywords:
Amycolatopsis methanolica
;
Mycobacterium gastri
;
Thiocarbamate
;
Triazine
;
Group III alcohol oxidoreductase
;
N,N′-Dimethyl-4-nitrosoaniline
;
Ethanol
;
Propionaldehyde
;
Ethanolamine
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A protein with a mol. mass of 51,000 (ThcE) that was induced in Rhodococcus sp. N186/21 during assimilation of thiocarbamate herbicides, atrazine, ethanol, propanol, glycerol, propionaldehyde or ethanolamine was identified by two-dimensional electrophoresis. The thcE gene was cloned and sequenced. The deduced amino acid sequence revealed ThcE as a member of group III alcohol dehydrogenases. ThcE displayed strong homology with sequenced subunit fragments of the homodecameric N,N′-dimethyl-4-nitrosoaniline-dependent alcohol oxidoreductases (MNO) of Amycolatopsis methanolica and Mycobacterium gastri. N-Terminal sequence analysis of purified MNO from Rhodococcus sp. NI86/21 confirmed the identity with ThcE. When overproduced in Escherichia coli, ThcE was insoluble and no MNO activity was detected.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00272133
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