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  • 1
    Electronic Resource
    Electronic Resource
    Minibeet initiation from derooted sugarbeet (Beta vulgaris L.) seedlings in vitro
    Amsterdam : Elsevier
    Plant Science 97 (1994), S. 217-224 
    Details
    ISSN: 0168-9452
    Keywords: Abscisic acid ; Beta vulgaris L ; Minibeet ; Polycambial structure ; Storage organ ; Sucrose accumulation
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0168-9452(94)90059-0
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  • 2
    Electronic Resource
    Electronic Resource
    Evidence for somatic embryogenesis during plant regeneration from seedling-derived callus of dodder (Cuscuta trifolii Bab. et Gibs) (2000)
    Springer
    Plant cell reports 19 (2000), S. 525-528 
    Details
    ISSN: 1432-203X
    Keywords: Key words Cuscuta trifolii ; Somatic embryogenesis ; Plant regeneration ; Parasitic weed
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  In this paper comparative histological studies of embryo-like structures originating from callus cultures, and zygotic embryos originating from sexual seeds of Cuscuta trifolii are reported. The embryos of somatic cell and zygote origin showed similar morphological and anatomical features, such as a complete lack of cotyledon development and the differentiation of a developmentally unique root primordium specialised for water storage. Based on these findings, the regeneration of C. trifolii from callus cultures is shown to proceed along the pathway of somatic embryogenesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050767
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  • 3
    Electronic Resource
    Electronic Resource
    Production of phosphinothricin-tolerant rice (Oryza sativa L.) through the application of phosphinothricin as growth regulator (2000)
    Springer
    Plant cell reports 19 (2000), S. 1226-1231 
    Details
    ISSN: 1432-203X
    Keywords: Keywords Rice ; Herbicide resistance ; In vitro selection ; Phosphinothricin ; Glutamine synthetase ; Abbreviationsbar: Bialaphos resistance gene ; GA3: Gibberellic acid ; GM: Germination medium (Toldi et al. 1994) ; GS: Glutamin synthetase ; MS: Murashige and Skoog (1962) medium ; N6: Chu (1978) medium ; PAT: Phosphinothricin acetyltransferase ; PPT: l-Phosphinothricin ; R0 plants: In vitro regenerated plants originating from tissue culture ; R1 plants: Progeny of R0 plants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A novel procedure has been developed to produce rice (Oryza sativa L.) tolerant to the herbicide phosphinothricin (PPT) by means of in vitro selection. First, sublethal and lethal concentrations of PPT on 7-day-old seedlings were determined and morphogenetic events in response to the PPT treatment evaluated. Differentiation of 6–30 microshoots on 5–40% of the treated plant material was observed on a hormone-free culture medium supplemented with a sublethal concentration of PPT. We proved that PPT is morphogenetically active, similar to the action of many other herbicides, showing cytokinin-like effects in rice tissue culture. Fertile plants were grown from those microshoots having PPT tolerance under greenhouse conditions. To the best of our knowledge, this is the first report on the production of rice plants tolerant to this herbicide without genetic transformation. Since PPT is a competitive inhibitor of glutamine synthetase (GS), total GS activity in PPT-tolerant and PPT-sensitive plants was examined comprehensively in order to decide whether this enzyme has any role in PPT tolerance. An elevated GS activity was detected in PPT-tolerant plant material which could result in an elevated PPT tolerance at unchanged concentrations of the herbicide.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990000276
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