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  • 1
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    Polylink: to support two-point linkage analysis in autotetraploids (2001)
    Oxford University Press
    Details
    Publication Date: 2001-08-01
    Print ISSN: 1367-4803
    Electronic ISSN: 1460-2059
    Topics: Biology , Computer Science , Medicine
    Published by Oxford University Press
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  • 2
    Electronic Resource
    Electronic Resource
    Incompatibility and resistance to woolly apple aphid in apple (2000)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 119 (2000), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The study investigated the reported linkage of the locus for resistance to woolly apple aphid with the locus for incompatibility. Apple seedlings from the cross ‘Northern Spy’(heterozygous for resistance) בTotem’(susceptible) were scored for resistance, and for incompatibility genotype, by analysis of stylar ribonucleases, and for Got-1, the isoenzyme marker for incompatibility. Cosegregation analysis provided no evidence that the loci for resistance and incompatibility are linked. Two rootstock cultivars,‘M9’and ‘Merton 789′, which in early work had been reported to give poor set in crosses with ‘Northern Spy’, were found to have the same incompatibility genotype as ‘Northern Spy’, namely S1S3.‘M4’and ‘Irish Peach’, two other cultivars that had given poor set when crossed on to ‘Northern Spy’, appeared to be homozygous at the incompatibility locus and to have the genotypes S3S3 and S1S1, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2000.00442.x
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  • 3
    Electronic Resource
    Electronic Resource
    Stylar ribonucleases in almond: correlation with and prediction of incompatibility genotypes (2003)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 122 (2003), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: To clarify incompatibility relationships among almond cultivars, 35 were analysed for stylar ribonucleases, which have previously been shown to correlate with incompatibility S alleles. Stylar proteins were extracted and separated electrophoretically and the zymograms compared with ladders of ribonucleases corresponding to the 12 S alleles previously reported. Sixteen cultivars showed a band corresponding to two of the known ribonucleases, 17 showed one known ribonuclease and one ‘new’ band, and two showed two new bands. Twelve new ribonucleases were detected; 11 were attributed to new S alleles (S13 to S23) and a mutant form of S7 was attributed to S7A. Genotypes were proposed for nine cultivars of five incompatibility groups that had not been genotyped previously, VII, X, XI, XII and XIII. Twenty-four cultivars of unknown incompatibility relationships were provisionally genotyped: six of these could be assigned to existing groups and two new groups were established, XIV and XV, along with group O of cultivars with unique genotypes. Test crosses confirmed that eight pairs of cultivars showing similar zymograms were indeed cross-incompatible, including the two representatives of each of the two new groups. Virtually all self-incompatible cultivars of known genotype are listed in a table. The data should be useful for planning cultivar combinations for orchards and for designing crosses for breeding programmes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2003.00744.x
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  • 4
    Electronic Resource
    Electronic Resource
    Determination of incompatibility genotypes in almond using first and second intron consensus primers: detection of new S alleles and correction of reported S genotypes (2005)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 124 (2005), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The work aimed to develop a reliable and convenient PCR approach for determining incompatibility S genotypes in almond. Initially, genomic DNAs of 24 accessions of known S genotype were amplified with novel consensus primers flanking the first and second introns of the S-RNase gene. The PCR products separated on agarose showed length polymorphisms and correlated well with the reference alleles S1-S23 and Sf. In addition, to improve discrimination between alleles of similar sizes, the same sets of primers but fluorescently labelled were used, and the products sized on an automated sequencer. These fluorescent primers were particularly informative in the case of the first intron, variation in the length of which has not been used previously for S genotyping in almond. Some reference alleles showed the same patterns with first and second intron primers, and others showed a microsatellite-like trace. Subsequently, the S genotypes of 26 cultivars not genotyped previously and of four of uncertain genotype were determined. An allele described in Australian work as putative S10 was shown to be a ‘new’ allele and ascribed to S24 and evidence of five more ‘new’S alleles was found, for which the labels S25-S29 are proposed. This PCR approach should be useful for genotyping in other Prunus crops.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.2004.01058.x
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  • 5
    Electronic Resource
    Electronic Resource
    Primers amplifying a range of Prunus S-alleles (2004)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 123 (2004), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Although various consensus polymerase chain reaction (PCR) primers have been reported for identifying Prunus S-alleles, they have been developed from and optimized on a limited set of alleles, which may limit their applicability to a broader allele range. To develop a primer set for use across the genus, degenerate consensus primers were designed from conserved regions of 27 S-RNase sequences available from five Prunus species. The primers were tested in 15 previously genotyped cultivars of cherry, almond and apricot, representing alleles S1 to S6 in each crop and also Sc in apricot. Comparisons were made with previously published primers tested in the same 15 cultivars under reported reaction conditions. The new primers generated an amplification product for each of the 19 S-alleles whereas those previously available amplified no more than 14. The primers will be useful for genotyping and genetic studies in cultivars and wild populations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.2004.01016.x
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  • 6
    Electronic Resource
    Electronic Resource
    Comparison of isoenzymes in Prunus avium separated by two different electrophoretic techniques (2000)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 119 (2000), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Isoenzyme variation for seven systems revealed by two different electrophoretic procedures was compared in Prunus avium. Fourteen cultivars and 14 wild selections were analysed for acid phosphatase (ACP), isocitrate dehydrogenase (IDH), leucine aminopeptidase (LAP), malate dehydrogenase (MDH), phosphoglucomutase (PGM), shikimate dehydrogenase (SKD) and superoxide dismutase (SOD). Extracts were separated by isoelectric focusing (IEF) and by polyacrylamide gel electrophoresis (PAGE). For the eight loci that had been described previously in these enzyme systems on the basis of IEF analysis, we compared the variation revealed with IEF and PAGE. Similar variation was revealed for Acp-1 and Pgm-1, and the alleles revealed by PAGE could be identified directly with those reported for IEF. For Lap-1, Mdh-1 and Skd-1, variation was seen with IEF but not with PAGE. For Mdh-2, PAGE revealed additional variation not revealed by IEF. For Idh-1, different patterns of variation were revealed by PAGE and IEF, and both procedures would be needed to genotype cherry accessions. We were unable to detect variation corresponding to that reported previously for Sod-1 with either technique. The implications of these findings for allele labelling, for studies of genetic diversity and for linkage analysis are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2000.00443.x
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  • 7
    Electronic Resource
    Electronic Resource
    A stylar ribonuclease assay to detect self-compatible seedlings in almond progenies (1999)
    Springer
    Theoretical and applied genetics 99 (1999), S. 800-810 
    Details
    ISSN: 1432-2242
    Keywords: Key words Almond ; Compatibility ; Genetics ; Prunus dulcis ; Ribonucleases
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Six almond progenies, each the product of a cross between a self-compatible and a self-incompatible parent, were analysed for stylar ribonucleases. Proteins were extracted and separated using non-equilibrium pH gradient electrofocusing (NEPHGE), and the gels were stained for ribonuclease activity. Most seedlings showed either two principal bands, interpreted as corresponding to two incompatibility alleles, or a single band. The seedlings were also bagged in the field at flowering time to determine fruit set after selfing, and some were also examined for the growth of pollen-tubes in selfed styles using UV fluorescence microscopy. With very few exceptions, those seedlings showing single-banded zymograms were found to be self-compatible according to field and microscope studies, and those with two bands were found to be self-incompatible. We conclude that the allele for self-compatibility in almond does not code for ribonuclease activity and that the ribonuclease isoenzyme assay is a convenient technique for predicting self-compatibility in segregating progenies. A novel band in two derivatives of ’Ferrastar’ was ascribed to a new incompatibility allele, S 10 .
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051299
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  • 8
    Electronic Resource
    Electronic Resource
    Re-examination of (in)compatibility genotypes of two John Innes self-compatible sweet cherry selections (2000)
    Springer
    Theoretical and applied genetics 101 (2000), S. 234-240 
    Details
    ISSN: 1432-2242
    Keywords: Key words Cherry ; Genetics ; Compatibility ; Incompatibility ; Isoelectric focusing ; Prunus avium ; Ribonuclease
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The (in)compatibility genotypes of two self-compatible sweet cherry selections, JI 2420 and JI 2434, originating from the John Innes Institute were re-examined. The selections and seedlings derived from them were analysed for stylar ribonucleases, which are known to correlate with S alleles, and the outcome of test crosses was recorded. JI 2420, which had been reported previously as S 3 S 4 ", where " indicates loss of pollen activity, was deduced to have the genotype S 4 S 4 ’. For JI 2434, which had been reported previously as S 3 S 4 0 , S 3 S 3 0 or S 3 S 3 ", where 0 indicates loss of pollen and stylar activity, two different clones were identified. One, at East Malling, was deduced to be S 3 "S 4 ; the other, at Ahrensburg, appeared to be S 3 S 3 " or S 3 S 3 0 .
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051474
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  • 9
    Electronic Resource
    Electronic Resource
    An isoenzyme marker linked to the incompatibility locus in cherry (2000)
    Springer
    Theoretical and applied genetics 100 (2000), S. 512-518 
    Details
    ISSN: 1432-2242
    Keywords: Key words Cherry ; Incompatibility ; Linkage ; Marker ; Prunus avium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Analysis of two cherry progenies from semi-compatible crosses for the esterase enzyme system showed extremely distorted segregation ratios for Est-5. Analysis of two progenies from compatible crosses for esterase and for stylar ribonuclease proved that Est-5 is linked with the incompatibility locus S. The recombination fraction is 4%. About a fifth of some 50 cultivars or selections genotyped for Est-5 were heterozygous. The various heterozygotes could provide ’testers’ for the presence in cultivars of unknown genotype of 8 of the 11 known S alleles. A seedling suitable for testing S 9 has been identified and crosses have been made to raise testers for S 10 and S 11 . Isoenzyme analysis of the four progenies for glutamate oxaloacetate transaminase, and of one of them for isocitrate dehydrogenase, showed no evidence for the linkage of Got-1 or Idh-2 with S, contrary to a previous report. Estimation of linkage with S in semi-compatible crosses is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050067
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  • 10
    Electronic Resource
    Electronic Resource
    Induction of tetraploidy in Buddleia globosa (2000)
    Springer
    Plant cell, tissue and organ culture 63 (2000), S. 121-125 
    Details
    ISSN: 1573-5044
    Keywords: Buddleia × weyeriana ; colchicine ; flow cytometry ; micropropagation ; ploidy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The aim was to produce a tetraploid form of Buddleia globosa to facilitate introgression of yellow flower colour into B. davidii, which is naturally tetraploid. Protocols were established for the micropropagation of B. globosa and tetraploid plants were obtained by application in vitro of colchicine to pre-cultured excised nodal sections. Three concentrations of colchicine were applied (0.01%, 0.05% and 0.1% w/v) for 1, 2 or 3 days. At 0.01% tetraploids were produced only after 2 days of application. All other treatments produced at least one tetraploid. The colchicine technique was extremely effective: of 29 lines tested, 19 were tetraploid and 5 were mixoploid. The vegetative characteristics of these tetraploids are described and the flowering characteristics of the three that flowered.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006434803003
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