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  • 1
    Electronic Resource
    Electronic Resource
    In situ formation of Y-Ba-Cu-O epitaxial films by 40.68-MHz rf magnetron sputtering (1991)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 69 (1991), S. 2731-2733 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: The in situ epitaxial growth of the Y-Ba-Cu-O films on MgO (001) was carried out by 40.68- MHz rf magnetron sputtering with an on-axis single target. It appeared that the rf discharge strongly affected film composition, crystallinity, superconductivity, and reproducibility. Highly orientated superconducting films with the c-axis perpendicular to the substrate surfaces and having zero resistance transition temperature of 78.2 K were obtained without a post-annealing.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.348628
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  • 2
    Electronic Resource
    Electronic Resource
    Regeneration of Oxalis triangularis ssp. triangularis from suspension cells cultured in three different systems (solid, liquid-flask and bioreactor cultures) (1999)
    Springer
    Plant cell reports 18 (1999), S. 701-706 
    Details
    ISSN: 1432-203X
    Keywords: Key words Activated Charcoal ; Airlift fermenter ; Bioreactor ; Liquid culture ; Organogenesis ; Oxalis triangularis ssp. triangularis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In attemps to establish in vitro cultures of Oxalis triangularis ssp. Triangularis, the explants of leaves, petioles, bulb scales and suspension cells derived from regenerated bulbs were examined using solid (petri dish), liquid-flask and bioreactor cultures. Only bulb-derived suspension cells were able to regenerate in all culture systems. The liquid-flask and bioreactor cultures supported organogenesis and yielded larger amount of buds than solid culture. The inclusion of AC in the culture medium delayed bud initiation but promoted plantlet development by minimising callusing of the buds. Morphological differences in regenerated plantlets affected by AC, such as the length and diameter of the petiole, leaf unfolding and the development of a red colour development on leaves and petioles, varied with the culture systems. Upon transfer to pots normal plants were recovered from buds regenerated in various culture systems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050646
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  • 3
    Electronic Resource
    Electronic Resource
    An alternative propagation method ofAnanas through nodule culture (1997)
    Springer
    Plant cell reports 16 (1997), S. 454-457 
    Details
    ISSN: 1432-203X
    Keywords: Bromeliad ; Direct regeneration ; Organogenesis ; Micropropagation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A micropropagation scheme forAnanas comosus Merr. was developed using nodule culture. Nodules were induced from leaf-base or chopped shoot-base explants on modified half-strength MS medium supplemented with 2.69-5.37 μM NAA and 4.44 μM BA and could be maintained long-term as nodules. The nodules proliferated into more nodules when chopped into pieces of 1–3 mm and placed onto the same medium. They regenerated shoots when transferred to medium supplemented with 0.54–10.74 μM NAA and 0.44–8.88 μM BA. The regeneration capacity of nodules is higher than that of direct regeneration or callus. Maximum regeneration was obtained from culture medium containing 0.54 μM NAA and 0.44 μM BA, where shoots could be observed as early as within 2 weeks. Many shoots formed roots in the same medium in which they were regenerated after 10 subcultures, but the best rooting occurred in medium containing 0.54 μM NAA and 0.44 μM BA. Rooted plantlets ofA. comosus Merr. could be routinely produced at 6-week intervals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01092765
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  • 4
    Electronic Resource
    Electronic Resource
    Source, etiolation and orientation of explants affect in vitro regeneration of Venus fly-trap (Dionaea muscipula) (1999)
    Springer
    Plant cell reports 18 (1999), S. 363-368 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsDionaea muscipula ; Venus fly-trap ; Tissue culture ; Organogenesis ; Micropropagation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In vitro culture of Venus fly-trap (Dionaea muscipula) was initiated using flower stalk explants. Activated charcoal was required for bud initiation, but omitted in the subculture of regenerated plantlets. Regenerated plants were subsequently used as explant source for investigations concerning effects of source of tissue, etiolation, orientation and illumination of leaf explants on plant regeneration. Etiolation of source plantlets increased the rate of regeneration from explants and decreased explant failure. Generally, adventitious buds developed at the adaxial side and proximal end of an explant. However, when explants were incubated in the dark, 20–30% of bud initiation occurred at the distal end. The site of shoot regeneration on a leaf explant was affected by both illumination and orientation of explants. Placing an explant adaxial side up resulted in the highest rate of regeneration. The most effective condition for plantlet regeneration was found with etiolated petioles incubated with the adaxial side facing the light.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050587
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  • 5
    Electronic Resource
    Electronic Resource
    Cryopreservation of Panax ginseng cells (2000)
    Springer
    Plant cell reports 19 (2000), S. 971-977 
    Details
    ISSN: 1432-203X
    Keywords: Key words Panax ginseng ; Suspension ; Cryopreservation ; Rapid freezing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The impacts of cryoprotectants (CP) and cell status during the growth cycle on Panax ginseng cell viability during cryopreservation were investigated. The ginseng cells used had a 5–7 times proliferation rate (compared with inoculum) in 2–3 weeks and were subcultured at 2- and 4-week intervals in liquid and on solid media, respectively. After testing various CP solutions of glycerol, dimethylsulphoxide, ethylene glycol and sucrose, a combination of 10% (v/v) glycerol and 4% (w/v) sucrose was selected for its least cytotoxicity and highest cell viability after thawing. With this CP solution, cells throughout the growth cycle exhibited a ‘U’-shaped fluctuation of post-thaw cell viability. The highest viability (86.5%) occurred during the lag phase from cells already maintained in suspension culture and then in the late exponential phase (61.7%); the lowest level of 15.4% was in the mid-exponential phase. Callus freshly transferred to liquid medium showed a less obvious fluctuation pattern. The recovered cells were brown-to-reddish at first and gradually returned to a light yellow colour after several subcultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990000212
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  • 6
    Electronic Resource
    Electronic Resource
    Activated charcoal affects morphogenesis and enhances sporophyte regeneration during leaf cell suspension culture of Platycerium bifurcatum (1997)
    Springer
    Plant cell reports 17 (1997), S. 77-83 
    Details
    ISSN: 1432-203X
    Keywords: Key words Staghorn fern ; Platycerium bifurcatum ; Organogenesis ; Gametophyte ; Hyperhydricity ; Activated charcoal ; Suspension culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In a leaf cell suspension culture of Platycerium bifurcatum, the incorporation of activated charcoal (AC) greatly increased the number of regenerated sporophytes even in growth regulator-free medium. The degree of improvement was dependent on cell aggregate sizes and medium composition. The maximal increase was observed in medium with 5.37 µM NAA and 4.44 µM BA, from 9 to 1520 sporophytes. The qualitative improvement by AC included: (1) regeneration of single sporophytes, which was relatively less frequent in non-AC media, (2) prevention of the formation of gametophyte clusters prior to sporophyte regeneration from 30- to 60-µm cells cultured in MS basal medium, (3) prevention of the formation of bud clusters, sporophytes with multiple bud primordia, `nodule'-like bud clusters in growth regulator containing media, and (4) prevention of the occurrence of hyperhydricity of regenerated sporophytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050356
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  • 7
    Electronic Resource
    Electronic Resource
    The impact of a pulse treatment of penicillin-G and streptomycin sulfate on sporophyte regeneration of Platycerium bifurcatum (2000)
    Springer
    Plant cell reports 19 (2000), S. 345-350 
    Details
    ISSN: 1432-203X
    Keywords: Key words Staghorn fern ; Platycerium bifurcatum ; Organogenesis ; Sporophyte ; Hyperhydricity ; Antibiotics ; Pulse treatment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  This study was aimed at investigating the impact of antibiotic pulse treatment (APT) of penicillin-G and streptomycin sulfate at a concentration of 1000 mg/l each on the regeneration ability of leaf and shoot-base explants and homogenized sporophytes of Platycerium bifurcatum. The reaction of the plant to APT, as measured by changes in sporophyte regeneration and morphology, was considered to be a stress response. Cell's susceptibility to APT was graded as: tissue homogenized prior to treatment〉tissue homogenized after treatment〉leaf and shoot-base explants. Biphasic inhibition in sporophyte regeneration was observed in homogenized sporophytes; a sharper decrease occurred with 20–60 min of the APT, followed by a slower decline for up to 180 min. No significant impact on the regeneration ability of leaf and shoot-base explants was found. Morphologically, APT caused leaf cleavage, mesophyll damage and hyperhydricity in some sporophytes. Except for the hyperhydric sporophytes, regenerants were able to continue their growth in soil, and no carry-over effect was found in the next generation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050738
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  • 8
    Electronic Resource
    Electronic Resource
    In vitro regeneration patterns of Platycerium bifurcatum leaf cell suspension culture (1997)
    Springer
    Plant cell reports 16 (1997), S. 820-824 
    Details
    ISSN: 1432-203X
    Keywords: Key words Staghorn fern ; Platycerium bifurcatum ; Apospory ; Apogamy ; Micropropagation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A simple suspension culture system of Platycerium bifurcatum was developed where sporophytes could be regenerated directly from leaf cells or indirectly through an aposporous gametophyte stage under the same culture conditions. Single cells and aggregates of up to 100 cells developed aposporous gametophytes which later gave rise to sporophytes. Such gametophytes started apogamy when they were mostly less than 0.7 mm in length, bearing only rhizoids. In most cases, only one sporophyte was regenerated from one gametophyte. Aggregates of 500–1000 or more cells, on the other hand, regenerated sporophytes directly. Intercellular interaction was considered to be the physiological cause, and the separation of leaf cells to a certain degree drove the cells to embark on different regeneration paths. It is suggested that the possible existence of a threshold size of cell aggregates separates the two regeneration patterns.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050327
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  • 9
    Electronic Resource
    Electronic Resource
    Micropropagation of Spathoglottis plicata (1997)
    Springer
    Plant cell reports 16 (1997), S. 831-835 
    Details
    ISSN: 1432-203X
    Keywords: Key words Organogenesis ; Micropropagation ; Protocorm-like-body ; Orchid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A rapid and reliable micropropagation method was established for Spathoglottis plicata. Nodal and leaf explants dissected from 8-month-old pot-grown seedlings were cultured on charcoal-amended Murashige and Skoog medium supplemented with 16 combinations of α-naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BA) at concentrations of 0.54–10.74 µm. Regeneration of protocorm-like bodies (PLBs) and subsequent plantlet development were observed from 98.5% of the nodal explants. Only 6.5% of leaf explants and occasionally some root segments (dissected from regenerated plantlets) were able to produce PLBs and then plantlets. The optimum plant growth regulator (PGR) combination for maximal PLB regeneration was 5.37 µm NAA and 0.44 µm BA. The best combination of PGR for plantlet development was 2.69–10.74 µm NAA and 8.88 µm BA. The NAA to BA ratios for maximal PLB induction and plantlet development were 12.2 and 0.3–1.2, respectively. Regenerated PLBs and plantlets, when cut into pieces of less than 1 mm and subcultured onto the above media, regenerated new PLBs and plantlets in another 3 months.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050329
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  • 10
    Electronic Resource
    Electronic Resource
    An alternative propagation method of Ananas through nodule culture (1997)
    Springer
    Plant cell reports 16 (1997), S. 454-457 
    Details
    ISSN: 1432-203X
    Keywords: Key words Bromeliad ; Direct regeneration ; Organogenesis ; Micropropagation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A micropropagation scheme for Ananas comosus Merr. was developed using nodule culture. Nodules were induced from leaf-base or chopped shoot-base explants on modified half-strength MS medium supplemented with 2.69–5.37 µM NAA and 4.44 µM BA and could be maintained long-term as nodules. The nodules proliferated into more nodules when chopped into pieces of 1–3 mm and placed onto the same medium. They regenerated shoots when transferred to medium supplemented with 0.54–10.74 µM NAA and 0.44–8.88 µM BA. The regeneration capacity of nodules is higher than that of direct regeneration or callus. Maximum regeneration was obtained from culture medium containing 0.54 µM NAA and 0.44 µM BA, where shoots could be observed as early as within 2 weeks. Many shoots formed roots in the same medium in which they were regenerated after 10 subcultures, but the best rooting occurred in medium containing 0.54 µM NAA and 0.44 µM BA. Rooted plantlets of A. comosus Merr. could be routinely produced at 6-week intervals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01092765
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