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  • 1
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Macromolecules 23 (1990), S. 2602-2604 
    ISSN: 1520-5835
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1520-5835
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1520-5835
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Physiology 35 (1973), S. 87-116 
    ISSN: 0066-4278
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Medicine , Biology
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Transient GUS (β-glucuronidase) expression was visualized in cell suspensions of Triticum aestivum, Zea mays, Pennisetum glaucum, Saccharum officinarum, Pennisetum purpureum and Panicum maximum after microprojectile bombardment with pBARGUS and pAHC25 plasmid DNAs. pBARGUS contains the GUS (UidA) gene coding region driven by the Adh1 promoter and the Adh1 intron 1, as well as the BAR gene coding region driven by the CaMV 35S promoter and the Adh1 intron 1. pAHC25 contains the GUS and BAR gene coding regions driven by the maize ubiquitin promoter, first exon and first intron (Ubi1). The effectiveness of the constructs was first compared in cell suspension cultures by counting blue expression units (b.e.u.). The expression of construct pAHC25 ranged from 3 to 50 fold greater than pBARGUS in different species. In addition, the two plasmids were quantitatively compared in Triticum aestivum and Zea mays by using the more sensitive GUS fluorometric assay to determine the amount of methylumbellyferride (MU) produced. There was more than a 30 fold increase in MU production with pAHC25 than with pBARGUS in the wheat suspension, while the maize suspension showed only a 2.5 fold increase with the pAHC25 construct. Transient GUS expression was also visualized in immature embryos of Pennisetum glaucum following bombardment with pBARGUS and pAHC25 DNA. Expression of plasmid pAHC25 was twice as high as pBARGUS. A comparison of two DNA/gold preparation methods, as well as repeated sonications of the DNA/gold mixture, had no effect on the number of b.e.u.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 74 (1987), S. 681-686 
    ISSN: 1432-2242
    Keywords: Cell cycle ; Embryogenic competence ; Nuclear DNA ; Pennisetum purpureum ; Tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mesophyll cell nuclei isolated from leaves of Pennisetum purpureum were analysed by flow cytometry to determine the nuclear DNA content and the percentage of cells in different phases of the cell cycle. Samples taken from base, middle and tip regions of leaves 2 to 8 (leaf 1, which was adjacent to the meristem, was too small to sample) showed no significant differences in the amount of DNA per G1 nucleus due to either age or position. The average amount of DNA per G1 nucleus was 5.78 pg. Although the majority of cells for each sample were in G1, samples taken from older leaves had higher percentages of cells in G2 and S phases. More specifically, base and middle regions of older leaves had a higher percentage of cells in G2 than all three positions in younger leaves. Electrophoretic analysis of nuclear DNA from leaves 2 to 7 showed no evidence of degradation or difference in fragment size for any sample or position. This study was compared to previous work on the relationship between leaf age and embryogenic competence in Pennisetum purpureum. The results suggest that changes in the cell cycle, and/or a loss or fragmentation of the nuclear DNA, are not responsible for loss of embryogenic competence in mature leaf tissue.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 41 (1985), S. 1407-1409 
    ISSN: 1420-9071
    Keywords: Mouse liver ; malaria infection ; benzoic acid detoxification ; glucuronic acid conjugation ; hippuric acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The metabolism of benzoic acid was studied inPlasmodium berghei infected mice both in vitro and in vivo. Results of in vitro studies showed a considerable decrease in the ability of the infected liver to detoxify benzoic acid by hippuric acid formation. The in vivo study showed that hippuric acid formation decreases with increasing parasitemia and the emergence of benzoyl-glucoronide. This new pathway stops operating with further increase in parasitemia.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 54 (1998), S. 196-202 
    ISSN: 1420-9071
    Keywords: Key words. Phosphatidyl serine; biomineralization; membrane structure; EXAFS; calcium binding.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. A phosphatidyl serine-amorphous calcium phosphate complex has been synthesized as a model of the matrix vesicle system that is associated with the induction of mineral deposition in bone, cartilage and dentine. The complex has been studied using a novel technique of subtractive extended X-ray absorption fine structure (EXAFS). This enables spectra of the components of the molecules to be subtracted from the complex so as to identify the sites of interaction. The results suggest there is a movement in the nitrogen atom of the phosphatidyl serine which approaches the calcium atom in the mineral phase. This interpretation would link the membrane structure of the vesicle to the structure of the mineral in a way that could explain some of its roles in biomineralization.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Medical & biological engineering & computing 16 (1978), S. 549-553 
    ISSN: 1741-0444
    Keywords: Blood velocity ; Directional Doppler ; Spectrum analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Description / Table of Contents: Sommaire Décrit deux dispositifs de traitement préliminaire qui peuvent tous deux être utilisés avant l'analyse spectrale pour afficher sans ambiguïté les formes d'onde directionnelle de vitesse du sang par un appareil d'analyse spectral. Les détails de circuits intéressants sont présentés.
    Abstract: Zusammenfassung Es werden zwei Vorverarbeitungsanlagen beschrieben, von denen jede vor der Spektralanalyse verwendet werden kann, damit der Spektrumanalysator keine unklaren Blutgeschwindigkeitswellenformen anzeigt. Es werden wichtige Einzelheiten des Schaltkreises dargestellt.
    Notes: Abstract Two processors are described, either of which may be used before spectral analysis to allow unambiguous display of directional blood-velocity waveforms by the spectrum analyser. The relevant circuit details are presented.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Medical & biological engineering & computing 16 (1978), S. 554-558 
    ISSN: 1741-0444
    Keywords: Measurement ; Strain gauges
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Description / Table of Contents: Sommaire L'appareil qui en a résulté, un pléthysmographe automatique à extensomètre à deux voies basé sur des techniques de mesure de résistance absolue, est décrit dans l'article. Il s'agit d'un apparei compact facile à utiliser, assurant un auto-calibrage inhérent pour n'importe quel extensomètre et quelle que soit sa longueur, qui effectue automatique la mesure, du cycle étudié et donne un tracé graphique annoté des données mesurées ainsi que les résultats qu'il calcule à partir desdites données.
    Abstract: Zusammenfassung Das Endresultat, ein Plethysmograph mit automatischem, zweikanaligen Dehnungsmeßgerät, der auf der absoluten Widerstandsmessungstechnik basiert, wird im folgenden beschrieben. Dieses Gerät ist kompakt, leicht zu handhaben, hat eine eingebaute Selbsteichung für jedes Meßgerät und jede Länge dieses Meßgeräts, führt einen automatischen Meßzyklus aus und erzeugt beschriftete, grafische Druckkopien der Meßdaten zusammen mit den Resultaten, die es aus diesen Daten berechnet.
    Notes: Abstract An automatic 2-channel strain-gauge plethysmograph, based on an absolute resistance measurement technique, will be described in the paper. This device is compact, easy to use, provides inherent self-calibration for any gauge and any length of that gauge, automatically performs the measured cycle, and generates annotated graphical printouts of the measurement data, along with the results which it computes from that data.
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