Publication Date:
1999-02-05
Description:
Stem cell homing and repopulation are not well understood. The chemokine stromal cell-derived factor-1 (SDF-1) and its receptor CXCR4 were found to be critical for murine bone marrow engraftment by human severe combined immunodeficient (SCID) repopulating stem cells. Treatment of human cells with antibodies to CXCR4 prevented engraftment. In vitro CXCR4-dependent migration to SDF-1 of CD34+CD38-/low cells correlated with in vivo engraftment and stem cell function. Stem cell factor and interleukin-6 induced CXCR4 expression on CD34+ cells, which potentiated migration to SDF-1 and engraftment in primary and secondary transplanted mice. Thus, up-regulation of CXCR4 expression may be useful for improving engraftment of repopulating stem cells in clinical transplantation.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Peled, A -- Petit, I -- Kollet, O -- Magid, M -- Ponomaryov, T -- Byk, T -- Nagler, A -- Ben-Hur, H -- Many, A -- Shultz, L -- Lider, O -- Alon, R -- Zipori, D -- Lapidot, T -- A130389/PHS HHS/ -- New York, N.Y. -- Science. 1999 Feb 5;283(5403):845-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Immunology, The Weizmann Institute of Science, Rehovot 76100, Israel.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9933168" target="_blank"〉PubMed〈/a〉
Keywords:
ADP-ribosyl Cyclase
;
Animals
;
Antibodies
;
*Antigens, CD
;
Antigens, CD34/analysis/immunology
;
Antigens, CD38
;
Antigens, Differentiation/analysis
;
Chemokine CXCL12
;
Chemokines, CXC/pharmacology/*physiology
;
Chemotaxis
;
Colony-Forming Units Assay
;
Fetal Blood
;
Hematopoietic Stem Cell Mobilization
;
*Hematopoietic Stem Cell Transplantation
;
Hematopoietic Stem Cells/*physiology
;
Humans
;
Interleukin-6/pharmacology
;
Membrane Glycoproteins
;
Mice
;
Mice, Inbred NOD
;
Mice, Knockout
;
Mice, SCID
;
NAD+ Nucleosidase/analysis
;
Receptors, CXCR4/biosynthesis/immunology/*physiology
;
Stem Cell Factor/pharmacology
;
Tetradecanoylphorbol Acetate/pharmacology
;
Up-Regulation
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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