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  • 1
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die zelluläre Verteilung von 134Cs in der Skelettmuskulatur von Mäusen wurde histoautoradiographisch untersucht. 4–6 Std nach einer intraperitonealen Applikation von 134CsCl zeigte sich eine starke Anhäufung der 134Cs-Aktivität im Bereich des Sarkolemms. Die Aktivität folgte den Konturen der Muskelzellmembran, ein Befund, der auf eine Adsorption der Cs-Ionen an das Sarkolemm schließen läßt. Innerhalb der Myofibrillen zeigte sich eine an die Querstreifung erinnernde Anordnung der Schwärzungen.
    Notes: Summary The cellular distribution of 134Cs in the skeletal muscle of mice was investigated by histoautoradiography. A relatively high accumulation of 134Cs was found at the sarcolemma 4–6 hours after intraperitoneal application of 134CsCl. This permits the conclusion that Cs-ions are probably adsorbed by the sarcolemma before they are moved into the muscle cell. Within the cell 134Cs seems to be linked with myofibrils at destinct distances similar to those between isotropic and anisotropic bands.
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  • 2
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The pre-epithelial mucus layer (PML) and epithelial mucins were studied by mucin histochemistry in 10μm-thick celloidinstabilized cryostat sections in the proximal and distal colon of conventional and germ-free rats aged 120 and 350 days. No continuous PML was found in the proximal colon. A continuous mucus blanket, of fairly homogenous thickness, was observed in the distal colon, where the PML-thickness was 40±24 μm at 120 days of age and 44±22μ at 350 days of age in conventional rats, and 25±17μm (120 days) and 22±10μ (350 days) in germ-free rats. The stainability of the PML by periodic acid-Schiff and Alcian Blue at pH 2.5 and 1.0 was stronger in conventional rats than in germ-free rats, indicating higher concentrations of mucosubstances and of acid and sulphated mucins, respectively. The PML of the conventional rat distal colon showed a stratified structure of up to eight sublayers. In the distal colon of germ-free rats, the whole gut wall thickness was reduced 47% compared to the conventional rat (germ-free: 185±73μm, conventional: 350±115μm). No stratification of the PML was observed. The presence of intestinal microflora obviously had a strong influence on the thickness, compactness, mucin content, mucin composition and structure of the pre-epithelial mucus layer.
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