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  • 1
    Publication Date: 2020-01-17
    Description: The Surface Ocean CO2 Atlas (SOCAT) is a synthesis activity by the international marine carbon research community (〉100 contributors). SOCAT version 4 has 18.5 million quality-controlled, surface ocean fCO2 (fugacity of carbon dioxide) observations with an accuracy of better than 5 µatm from 1957 to 2015 for the global oceans and coastal seas. Automation of data upload and initial data checks speeds up data submission and allows annual releases of SOCAT from version 4 onwards. SOCAT enables quantification of the ocean carbon sink and ocean acidification and evaluation of ocean biogeochemical models. SOCAT represents a milestone in research coordination, data access, biogeochemical and climate research and in informing policy.
    Type: Dataset
    Format: application/zip, 1265 datasets
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  • 2
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    PANGAEA
    In:  Supplement to: Bakker, Dorothee C E; O'Brien, Kevin M; Pfeil, Benjamin; Currie, Kim I; Kozyr, Alexander; Landa, Camilla S; Lauvset, Siv K; Metzl, Nicolas; Nakaoka, Shin-Ichiro; Nojiri, Yukihiro; Nonaka, Isao; Olsen, Are; Omar, Abdirahman M; Pierrot, Denis; Saito, Shu; Smith, Karl; Sutton, Adrienne; Sullivan, Kevin; Tilbrook, Bronte; Wanninkhof, Rik; Akl, John; Alin, Simone R; Barbero, Leticia; Barrera, Kira E; Beaumont, Laurence; Becker, Meike; Bernard, Christophe; Bott, Randy; Byrne, Robert; Cai, Wei-Jun; Cosca, Catherine E; Cross, Jessica; Daly, Kendra L; Danguy, Théo; De Carlo, Eric Heinen; Dietrich, Colin; Feely, Richard A; Fiedler, Björn; Glockzin, Michael; Gove, Matthew D; Goyet, Catherine; Guillot, Antoine; Hales, Burke; Hartman, Sue E; Herndon, Julian; Hoppema, Mario; Humphreys, Matthew P; Hunt, Christopher W; Huss, Betty; Hydes, David; Ibánhez, J Severino P; Ishii, Masao; Johannessen, Truls; Jones, Steve D; Kitidis, Vassilis; Knorr, Paul O; Körtzinger, Arne; Kosugi, Naohiro; Lee, Charity M; Lefèvre, Nathalie; Lo Monaco, Claire; Liu, Xuewu; Maenner, Stacy M; Manke, Ansley; Manzello, Derek P; Mathis, Jeremy T; Mickett, John; Millero, Frank J; Monacci, Natalie; Monteiro, Pedro; Morell, Julio; Munro, David R; Musielewicz, Sylvia; Neill, Craig; Newberger, Timothy; Newton, Jan; Noakes, Scott; Noh, Jae Hoon; Ohman, Mark; Ólafsdóttir, Sólveig Rósa; Ólafsson, Jón; Osborne, John; Padín, Xose Antonio; Rehder, Gregor; Reimer, Janet J; Robbins, Lisa L; Rutgersson, Anna; Sabine, Christopher L; Salisbury, Joe; Sasano, Daisuke; Schlitzer, Reiner; Schuster, Ute; Send, Uwe; Sieger, Rainer; Skjelvan, Ingunn; Steinhoff, Tobias; Sutherland, Stewart C; Sweeney, Colm; Takahashi, Taro; Telszewski, Maciej; Vandemark, Doug; van Heuven, Steven; Wallace, Douglas WR; Woosley, Ryan; Wynn, Jonathan G; Yates, Kimberly Kaye (in prep.): Version 5 of the Surface Ocean CO2 Atlas (SOCAT).
    Publication Date: 2020-01-17
    Description: The Surface Ocean CO2 Atlas (SOCAT) is a synthesis activity by the international marine carbon research community (〉100 contributors). SOCAT version 5 has 21.5 million quality-controlled, surface ocean fCO2 (fugacity of carbon dioxide) observations from 1957 to 2017 for the global oceans and coastal seas. Calibrated sensor data are also available. Automation allows annual, public releases. SOCAT data is discoverable, accessible and citable. SOCAT enables quantification of the ocean carbon sink and ocean acidification and evaluation of ocean biogeochemical models. SOCAT, which celebrates its 10th anniversary in 2017, represents a milestone in biogeochemical and climate research and in informing policy.
    Type: Dataset
    Format: application/zip, 823 datasets
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  • 3
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    PANGAEA
    In:  Supplement to: Bakker, Dorothee C E; Pfeil, Benjamin; Landa, Camilla S; Metzl, Nicolas; O'Brien, Kevin M; Olsen, Are; Smith, Karl; Cosca, Catherine E; Harasawa, Sumiko; Jones, Steve D; Nakaoka, Shin-Ichiro; Nojiri, Yukihiro; Schuster, Ute; Steinhoff, Tobias; Sweeney, Colm; Takahashi, Taro; Tilbrook, Bronte; Wada, Chisato; Wanninkhof, Rik; Alin, Simone R; Balestrini, Carlos F; Barbero, Leticia; Bates, Nicolas R; Bianchi, Alejandro A; Bonou, Frédéric Kpédonou; Boutin, Jacqueline; Bozec, Yann; Burger, Eugene; Cai, Wei-Jun; Castle, Robert D; Chen, Liqi; Chierici, Melissa; Currie, Kim I; Evans, Wiley; Featherstone, Charles; Feely, Richard A; Fransson, Agneta; Goyet, Catherine; Greenwood, Naomi; Gregor, Luke; Hankin, Steven; Hardman-Mountford, Nicolas J; Harlay, Jérôme; Hauck, Judith; Hoppema, Mario; Humphreys, Matthew P; Hunt, Christopher W; Huss, Betty; Ibánhez, J Severino P; Johannessen, Truls; Keeling, Ralph F; Kitidis, Vassilis; Körtzinger, Arne; Kozyr, Alexander; Krasakopoulou, Evangelia; Kuwata, Akira; Landschützer, Peter; Lauvset, Siv K; Lefèvre, Nathalie; Lo Monaco, Claire; Manke, Ansley; Mathis, Jeremy T; Merlivat, Liliane; Millero, Frank J; Monteiro, Pedro M S; Munro, David R; Murata, Akihiko; Newberger, Timothy; Omar, Abdirahman M; Ono, Tsuneo; Paterson, Kristina; Pearce, David J; Pierrot, Denis; Robbins, Lisa L; Saito, Shu; Salisbury, Joe; Schlitzer, Reiner; Schneider, Bernd; Schweitzer, Roland; Sieger, Rainer; Skjelvan, Ingunn; Sullivan, Kevin; Sutherland, Stewart C; Sutton, Adrienne; Tadokoro, Kazuaki; Telszewski, Maciej; Tuma, Matthias; van Heuven, Steven; Vandemark, Doug; Ward, Brian; Watson, Andrew J; Xu, Suqing (2016): A multi-decade record of high-quality fCO2 data in version 3 of the Surface Ocean CO2 Atlas (SOCAT). Earth System Science Data, 8(2), 383-413, https://doi.org/10.5194/essd-8-383-2016
    Publication Date: 2020-01-17
    Description: The Surface Ocean CO2 Atlas (SOCAT) is a synthesis of quality-controlled fCO2 (fugacity of carbon dioxide) values for the global surface oceans and coastal seas with regular updates. Version 3 of SOCAT has 14.5 million fCO2 values from 3646 data sets covering the years 1957 to 2014. This latest version has an additional 4.4 million fCO2 values relative to version 2 and extends the record from 2011 to 2014. Version 3 also significantly increases the data availability for 2005 to 2013. SOCAT has an average of approximately 1.2 million surface water fCO2 values per year for the years 2006 to 2012. Quality and documentation of the data has improved. A new feature is the data set quality control (QC) flag of E for data from alternative sensors and platforms. The accuracy of surface water fCO2 has been defined for all data set QC flags. Automated range checking has been carried out for all data sets during their upload into SOCAT. The upgrade of the interactive Data Set Viewer allows better interrogation of the SOCAT data collection and rapid creation of high-quality figures for scientific presentations. Automated data upload has been launched for version 4 and will enable more frequent SOCAT releases in the future. High-profile scientific applications of SOCAT include quantification of the ocean sink for atmospheric carbon dioxide and its long-term variation, detection of ocean acidification, as well as evaluation of coupled-climate and ocean-only biogeochemical models. Users of SOCAT data products are urged to acknowledge the contribution of data providers, as stated in the SOCAT Fair Data Use Statement. This living data publication documents changes in the methods and data sets used in this new version of the SOCAT data collection compared with previous publications of this data collection (Pfeil et al., 2013; Sabine et al., 2013; Bakker et al., 2014).
    Type: Dataset
    Format: application/zip, 3657 datasets
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  • 4
    Publication Date: 2020-01-17
    Description: The Surface Ocean CO2 Atlas (SOCAT) is a synthesis activity by the international marine carbon research community (〉100 contributors). SOCAT version 6 has 23.4 million quality-controlled, surface ocean fCO2 (fugacity of carbon dioxide) observations from 1957 to 2017 for the global oceans and coastal seas. Calibrated sensor data are also available. Automation allows annual, public releases. SOCAT data is discoverable, accessible and citable. SOCAT enables quantification of the ocean carbon sink and ocean acidification and evaluation of ocean biogeochemical models. SOCAT represents a milestone in biogeochemical and climate research and in informing policy.
    Type: Dataset
    Format: application/zip, 424 datasets
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  • 5
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    Saranac Lake, N.Y., etc. : Periodicals Archive Online (PAO)
    Management Review. 77:6 (1988:June) 46 
    ISSN: 0025-1895
    Topics: Economics
    Description / Table of Contents: SPECIAL ISSUE ON SALES AND MARKETING
    Notes: Features
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  • 6
    ISSN: 1617-4623
    Keywords: Restriction-modification system ; Neisseria gonorrhoeae ; Nucleotide sequence of R.-M. genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The NgoPII restriction endonuclease, which recognizes the sequence 5′-GG↓CC-3′, differs from its isoschizomer HaeIII in being sensitive to methylation at the external cytosine residue. The entire nucleotide sequence of a cloned 3.3 kb segment of Neisseria gonorrhoeae strain P9 chromosomal DNA which harbours the NgoPII restriction-modification system has been determined. This data, coupled with sub-cloning experiments, indicates that the restriction endonuclease (R.NgoII) and modification (M.NgoII) genes are transcribed from separate promoters but are arranged in tandem, with the R.NgoPII gene being located on the 5′ side of the M.NgoPII gene. Unlike all previously reported restriction systems the 3′ end of the endonuclease open reading frame overlaps the 5′ end of the methylase open reading frame by 8 codons. This overlap may have implications for the regulation of the NgoPII restriction-modification system.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 0173-0835
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Part of the human mitochondrial D-loop region was amplified by two successive rounds of polymerase chain reaction (PCR) amplification. In the second PCR reaction, nested primers were used, of which one contained the M 13-21 universal primer sequence. By using nonequal concentrations of primers in the second amplification, single-stranded DNA was generated. This was then sequenced directly by the dideoxy chain termination method using dye-labelled universal sequencing primers in conjunction with a fluorescence-based DNA sequencer. This enabled a 403-base-pair hypervariable segment of the D-loop region to be readily sequenced in a single reaction. This paper describes a protocol which enables mitochondrial sequence information to be generated rapidly and automatically. It is likely to be of importance in forensic analysis where the DNA is too degraded or of insufficient quantity to be analysed by other techniques.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Electrophoresis 13 (1992), S. 173-175 
    ISSN: 0173-0835
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: DNA profiling analysis of preserved tissue samples was achieved by using an extraction method which incorporated the use of a chelating resin “Chelex” to chelate inhibitory polyvalent metal ions. An automated sequencer was used to estimate sizes of low molecular weight trimeric and tetrameric microsatellites labelled with fluorescent dyes. The entire analysis can be completed within 48h. Probabilities of chance association using two microsatellites were calculated as ranging between 10-2 and 10-4. The methods described have considerable potential for use in routine genetic analysis of preserved samples.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract CENP-B is a centromere associated protein originally identified in human cells as an 80 kDa autoantigen recognized by sera from patients with anti-centromere antibodies (ACA). Recent evidence indicates that CENP-B interacts with centromeric heterochromatin in human chromosomes and may bind to a specific subset of human alphoid satellite DNA. CENP-B has not been unambiguously identified in non-primates and could, in principal, be a primate-specific alphoid DNA binding protein. In this work, a human genomic DNA segment containing the CENP-B gene was isolated and subjected to DNA sequence analysis. In vitro expression identified the site for translation initiation of CENP-B, demonstrating that it is encoded by an intronless open reading frame (ORF) in human DNA. A homologous mouse gene was also isolated and characterized. It was found to possess a high degree of homology with the human gene, containing an intronless ORF coding for a 599 residue polypeptide with 96% sequence similarity to human CENP-B. 5′ and 3′ flanking and untranslated sequences were conserved at a level of 94.6% and 82.7%, respectively, suggesting that the regulatory properties of CENP-B may be conserved as well. CENP-B mRNA was detected in mouse cells and tissues and an immunoreactive nuclear protein identical in size to human CENP-B was detected in mouse 3T3 cells using human ACA. Analysis of the sequence of CENP-B revealed a segment of significant similarity to a DNA binding motif identified for the helix-loop-helix (HLH) family of DNA binding proteins. These data demonstrate that CENP-B is a highly conserved mammalian protein that may be a member of the HLH protein family and suggest that it plays a role in a conserved aspect of centromere structure or function.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A computerised system has been used to store DNA profiles from 3 hypervariable loci. This initial survey illustrates that band matching is only possible after analysis of the errors associated with electrophoretic systems. A number of databases have been constructed with the three probes investigated and two methods of frequency determination, ‘binning’ and ‘sliding window’ fitting, have been compared.
    Type of Medium: Electronic Resource
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