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  • 1
    ISSN: 0378-1119
    Keywords: Ap^r, Ap^s, ampicillin-resistant, -sensitive ; Cloning ; Cm^r, chloramphenicol-rcsistant ; Km^r, Km^s, kanamycin-resistant, -sensitive ; Tn5 transposon ; U, units ; UV, ultraviolet ; excision repair ; kb, kilobase pairs ; maxicells ; single-strand DNA-binding protein ; ts, thermosensitive
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Soil Science Society of America journal 63 (1999), S. 1763-1777 
    ISSN: 1435-0661
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract All strains of Comamonas testosteroni investigated here, produced quinohaemoprotein ethanol dehydrogenase (QH-EDH) when grown on ethanol or butanol, but one strain of C. acidovorans and of C. terrigena did not. Hybridization experiments showed that the gene for QH-EDH is absent in the latter two strains. Induction and properties of the QH-EDHs seem to be similar: all C. testosteroni strains produced the enzyme in its apo-form [without pyrroloquinoline quinone (PQQ)] and the levels were higher at growth at low temperature; preference for the R-enentiomer and similar selectivity was shown in the oxidation of solketal (2,2-dimethyl-1,3-dioxolane-4-methanol) by cells (supplemented with PQQ); the fragment of the qhedh gene gave high hybridization with the DNA of the C. testosteroni strains. Experiments with C. testosteroni LMD 26.36 revealed that the organism is well suited for production of (S)-solketal: it shows an adequate enantioselectivity (E value of 49) for the oxidation of racemic solketal; the conversion rate of (R)-solketal is only 3.5 times lower than that of ethanol; the optimal pH for conversion (7.6) is in a region where solketal has sufficient chemical stability; separation of the remaining (S)-solketal from the acid formed is simple; induction of QH-EDH, the sole enzyme responsible for the oxidation of (R)-solketal, occurs during growth on ethanol or butanol so that the presence of solketal (inhibitory for growth) is not required; production of active cells and the conversion step can be integrated into one process, provided that PQQ and solketal addition occur at the appropriate moment; the conversion seems environmentally feasible. However, since high concentrations of solketal inhibit respiration via QH-EDH, further investigations on the mechanism of inhibition and the stability of the enzyme might be rewarding as it could lead to application of higher substrate concentrations with consequently lower down-stream processing costs.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract All strains of Comamonas testosteroni investigated here, produced quinohaemoprotein ethanol dehydrogenase (QH-EDH) when grown on ethanol or butanol, but one strain of C. acidovorans and of C. terrigena did not. Hybridization experiments showed that the gene for QH-EDH is absent in the latter two strains. Induction and properties of the QH-EDHs seem to be similar: all C. testosteroni strains produced the enzyme in its apo-form [without pyrroloquinoline quinone (PQQ)] and the levels were higher at growth at low temperature; preference for the R–enantiomer and similar selectivity was shown in the oxidation of solketal (2,2-dimethyl-1,3-dioxolane-4-methanol) by cells (supplemented with PQQ); the fragment of the qhedh gene gave high hybridization with the DNA of the C. testosteroni strains. Experiments with C. testosteroni LMD 26.36 revealed that the organism is well suited for production of (S)-solketal: it shows an adequate enantioselectivity (E value of 49) for the oxidation of racemic solketal; the conversion rate of (R)-solketal is only 3.5 times lower than that of ethanol; the optimal pH for conversion (7.6) is in a region where solketal has sufficient chemical stability; separation of the remaining (S)-solketal from the acid formed is simple; induction of QH-EDH, the sole enzyme responsible for the oxidation of (R)-solketal, occurs during growth on ethanol or butanol so that the presence of solketal (inhibitory for growth) is not required; production of active cells and the conversion step can be integrated into one process, provided that PQQ and solketal addition occur at the appropriate moment; the conversion seems environmentally feasible. However, since high concentrations of solketal inhibit respiration via QH-EDH, further investigations on the mechanism of inhibition and the stability of the enzyme might be rewarding as it could lead to application of higher substrate concentrations with consequently lower downstream processing costs.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Nutrient cycling in agroecosystems 35 (1993), S. 227-235 
    ISSN: 1573-0867
    Keywords: integrated nutrient management systems ; land use systems ; mineral fertilizers ; manure ; nutrient balance ; sub-Saharan Africa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Nutrient balances were calculated for the arable soils of 38 sub-Saharan African countries. FAO production figures and forecasts for 35 crops for the period 1982–1984 and for 2000 were used to define land use systems, further characterized by fertility input through fertilizers, manure, rain and dust, biological N-fixation, and sedimentation, and fertility output through harvest of crops and removal of residues, leaching, denitrification, and erosion. The summarized output of the study is the sum of inputs minus the sum of outputs of nitrogen, phosphorus and potassium in the root zone. The alarming annual average nutrient loss for sub-Saharan Africa was 22 kg N, 2.5 kg P, and 15 kg K in 1982–84, and will be 26 kg N, 3 kg P, and 19 kg K in 2000. As the soil nutrient pool has to offset the negative balances each year, there is gross nutrient mining in sub-Saharan Africa. The need for integrated systems of nutrient management is emphasized, manipulating all inputs and outputs in a judicious way. Future scenarios of ‘continued mining’ and ‘conservation of soil fertility’ are discussed.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Nutrient cycling in agroecosystems 35 (1993), S. 237-250 
    ISSN: 1573-0867
    Keywords: denitrification ; erosion ; integrated nutrient management ; Kenya ; land use system ; leaching ; manure ; mineral fertilizers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In a recent study on the NPK balance of land use systems in sub-Saharan Africa, it was found that scale-inherent simplifications were inevitable (Stoorvogel et al., 1993). This article reports on a similar exercise in a well-inventorized smaller area (Kisii District, Southwestern Kenya). Land use types and land/water classes (combinations of rainfall zones and soil units) were combined into geographically well-defined land use systems with NPK inputs by mineral fertilizers, manure, wet and dry deposition, and biological N fixation, and outputs by aboveground crop parts, leaching, denitrification, and erosion. Primary data were available on applied mineral fertilizers and manure, crop yields, nutrient contents, residue removal and erosion. Deposition, leaching and denitrification were estimated using rainfall, clay, N and K content, and fertilizer input. Erosion was estimated along the lines of the Universal Soil Loss Equation. The aggregated nutrient balance for the Kisii District was -112kgN, -3kgP, and-70kgKha−1yr−1. For all nutrients, removal of harvested product was the strongest negative contributor, followed by erosion. In terms of land use, nutrient depletion was highest under pyrethrum and lowest under tea. Sensitivity analysis revealed that changing mineralization rate and soil N content had an important impact on the N balance. Varying slope gradient and length, soil erodibility, land cover and the enrichment factor for eroded material affected all nutrients. Examples are given of possible ways to improve the NPK balance in the Kisii District by manipulating inputs and outputs. The methodology can prove valuable in any area where the farming community is receptive to integrated nutrient management systems.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Precision agriculture 2 (2000), S. 319-332 
    ISSN: 1573-1618
    Keywords: precision agriculture ; nitrogen fertilization ; real-time modeling ; management units
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Nitrogen (N) emissions to ground and surface waters have become a major concern in many regions. In reaction, policy makers are tightening environmental constraints on agriculture, resulting in a call for more efficient management systems. This study presents a methodology for precision N fertilization in high-input farming systems applying split fertilizer strategies. Essentially, the method uses a mechanistic simulation model to quantify (i) soil mineral-N levels and (ii) N uptake rates on a real-time basis. Early warning signals are generated once N concentrations drop below a critical threshold level, indicating that additional fertilizer should be applied. Thresholds are not static, but defined in relation to actual uptake rates. Spatial variation is incorporated through the concept of management units: i.e., stable units with relatively homogeneous characteristics in terms of water regimes and nutrient dynamics. Separate simulations are conducted for each management unit, based on selected representative soil profiles. The proposed methodology was tested in a winter wheat (Triticum aestivum L.) field during the 1998 growing season. Six experimental strips were delineated receiving either ‘precise’ or traditional fertilization. Precision fertilization proved efficient in reducing fertilizer inputs (−23%), while slightly improving grain yields (+3%) and hectoliter weights (+4%). Results clearly illustrate the significance of precision management in the process of increasing fertilizer use efficiency.
    Type of Medium: Electronic Resource
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  • 8
    Publication Date: 1992-03-01
    Print ISSN: 0361-5995
    Electronic ISSN: 1435-0661
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Wiley
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  • 9
    Publication Date: 1999-11-01
    Print ISSN: 0361-5995
    Electronic ISSN: 1435-0661
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Wiley
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  • 10
    Publication Date: 2018-04-16
    Print ISSN: 1385-2256
    Electronic ISSN: 1573-1618
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Springer
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