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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 85 (1992), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A highly efficient method of regenerating fertile, phenotypically normal plants from shoot apex cultures of T. aestivum was developed. The hypodermal layer (L2) of the vegetative apex containing germ line precursor cells could be located with bright field microscopy and targeted for microinjection. Fluorescently labelled dextrans were used as markers to develop a microinjection procedure which did not disrupt nuclear or cytoplasmic structure. This procedure was used to inject plasmid DNA into L2 cells. Capillary microinjection did not shear the plasmid DNA and delivery of DNA was confirmed by polymerase chain reaction analysis of DNA isolated from injected apices. The significance of these findings in relation to the development of cereal transformation systems will be discussed.
    Type of Medium: Electronic Resource
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  • 2
    Publication Date: 2019-06-28
    Description: The data quality analysis program that was developed at Langley Research Center's National Transonic Facility is described. The program provides a computer driven systematic analysis of data taken during calibrations of the high speed digital data acquisition system. Five distinct checks that are performed on the calibration data are outlined. The five checks are for non-linearity, noise, short term drift, long term drift, and the proper functioning of the calibrator. The program has established a standard set of evaluation guidelines.
    Keywords: COMPUTER PROGRAMMING AND SOFTWARE
    Type: NASA-CR-4303 , NAS 1.26:4303
    Format: application/pdf
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  • 3
    Publication Date: 2019-07-17
    Description: Parameterizations of the frontal area index and canopy area index of natural or randomly distributed plants are developed, and applied to the estimation of local aerodynamic roughness using satellite imagery. The formulas are expressed in terms of the subpixel fractional vegetation cover and one non-dimensional geometric parameter that characterizes the plant's shape. Geometrically similar plants and Poisson distributed plant centers are assumed. An appropriate averaging technique to extend satellite pixel-scale estimates to larger scales is provided. The parameterization is applied to the estimation of aerodynamic roughness using satellite imagery for a 2.3 sq km coniferous portion of the Landes Forest near Lubbon, France, during the 1986 HAPEX-Mobilhy Experiment. The canopy area index is estimated first for each pixel in the scene based on previous estimates of fractional cover obtained using Landsat Thematic Mapper imagery. Next, the results are incorporated into Raupach's (1992, 1994) analytical formulas for momentum roughness and zero-plane displacement height. The estimates compare reasonably well to reference values determined from measurements taken during the experiment and to published literature values. The approach offers the potential for estimating regionally variable, vegetation aerodynamic roughness lengths over natural regions using satellite imagery when there exists only limited knowledge of the vegetated surface.
    Keywords: Earth Resources and Remote Sensing
    Type: Dec 06, 1998 - Dec 10, 1998; San Francisco, CA; United States
    Format: text
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