ISSN:
0951-4198
Keywords:
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Physics
Notes:
Structures of the trifunctional crosslink molecules, pyridinoline, deoxypyridinoline and glucosylgalactosyl-pyridinoline, have been characterized by positive-ion liquid secondary-ion mass spectrometry (LSIMS) and collision-induced dissociation with scanning-array four-sector tandem mass spectrometry. It has been found that: (i) the naturally occurring cations (Cat+) are readily desorbed by positive-ion LSIMS utilizing glycerol + thioglycerol (1:1) as liquid matrix and dominate the spectra, facilitating accurate mass measurement; (ii) changing the matrix to 3-nitrobenzyl alcohol leaves little evidence of the cation, and (iii) collision-induced dissociation of the cations (Cat+) of pyridinoline, deoxypyridinoline and glucosylgalatosyl-pyridinoline gives a wealth of fragmentation for structure elucidation. These fundamental studies of tandem mass spectra of the known trifunctional crosslink molecules provided valuable structure-related information, which help in the identification of the tissue-specific peptides containing these crosslinks. Artefact ions in these tandem mass spectra are remarkably abundant.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/rcm.1290080119
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