ISSN:
1573-5028
Keywords:
upstream ORF
;
regulated splicing
;
myb
;
stress
;
leucine zipper
;
plant
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract We have isolated two overlapping cDNAs coding for a MYB-related protein expressed in aerobic and anaerobic rice (Oryza sativa) roots and coleoptiles. Analysis of their sequences reveals some peculiar features, suggesting the presence of post-transcriptional regulation events: an upstream ORF, two unspliced introns and a putative leucine zipper in the ORF coded by the unspliced RNA. Transient expression in protoplasts indicates that the upstream ORF inhibits expression of a downstream coding sequence. Finally, we demonstrated that anoxia, in roots, increases the ratio between the spliced and the unspliced mRNA and affects the expression of other myb-related genes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1005821518242
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