ISSN:
0006-3592
Keywords:
chemostat
;
enzyme overproduction
;
plasmid stability
;
Escherichia coli
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
The effect of plasmid-mediated metabolic burden of on the expression of the host genes and its consequences on the plasmid maintenance were studied in carbon-limited chemostat culture of Escherichia coli 1EA(pBR322) subject to selection for strains overproducing chromosomally coded ribitol dehydrogenase. The chemostat population became rapidly heterogeneous and the competition among evolved strains was found to be crucial for the kinetics of the plasmid loss from the culture. The selective disadvantages in growth rate associated with plasmid carriage in the parent-like and ribitol dehydrogenase-overproducing strains was estimated. © 1993 John Wiley & Sons, Inc.
Additional Material:
3 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bit.260410306
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